LOCUS       sequence-167047-       11494 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  target  miRNA164  for destruction in Arabidopsis thaliana.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11494)
  AUTHORS   Tang G, Yan J, Gu Y, Qiao M, Fan R, Mao Y, Tang X
  TITLE     Construction of short tandem target mimic (STTM) to block the 
            functions of plant and animal microRNAs.
  JOURNAL   Methods. 2012 Oct;58(2):118-25. doi: 10.1016/j.ymeth.2012.10.006. 
            Epub 2012 Oct 23.
  PUBMED    23098881
REFERENCE   2  (bases 1 to 11494)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ymeth.2012.10.006"; journalName: "Methods"; date: 
            "2012-10"; volume: "58"; issue: "2"; pages: "118-25"
COMMENT     Sequence Label: pFGC5941DR5-PacI-ath-STTM164
FEATURES             Location/Qualifiers
     source          1..11494
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(472..491)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(473..491)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        682..1062
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     CDS             1068..1619
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     terminator      1629..1881
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     misc_feature    1967..1991
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             2416..3210
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      3297..3885
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4039..4056
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    4071..4211
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(4137..4156)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4297..4319
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      4555..4749
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(4815..5888)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(6317..6946)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    8247..8271
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     8459..8481
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8473..8490
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8474..8490
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        join(9484..9489,9490..9506,9507..9512)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     CDS             9549..10208
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKEITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(9615..9634)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        10279..10297
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     10279..10296
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        complement(join(11214..11494,1..65))
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
ORIGIN
        1 gacaggtagc tgggcaatgg aatccgagga ggtttcccga aattaccctt tgttgaaaag
       61 tctcaatagc cctttggtct tctgagacag tatctttgac atttttggag taggggcggg
      121 attgtgcgtc atcccttacg tcagtggaga tgtcacatca atccacttgc tttgaagacg
      181 tggttggaac gtcttctttt cccacgatgc tcctcgtggg tgggggtcca tctttgggac
      241 cactgtcggc agaggcatct tgaatgatag cctttccttt atcgcaatga tggcatttgt
      301 aggagccacc ttccttttcc actgtccttt cgatgaagtg acagatagct gggcaatgga
      361 atccgaggag gtttcccgaa attacccttt gttgaaaagt ctcaatagcc ctttggtctt
      421 ctgagactgt atctttgaca tttttggagt agggatcaga tctaccggtc tccctatagt
      481 gagtcgtatt aatttcgata agccaggtta acctgcatta atgaatcggc tgcagtaccc
      541 gggaatttaa cccgcctaat gagcgggctt ttttttgtga tccaaaggat ctccttgaga
      601 tccttttttt ctgcgcgtaa tctgctgctt gcaaacttaa ttaactctct agactcacct
      661 aggatccatg aattctgaga tttttcaaat cagtgcgcaa gacgtgacgt aagtatccga
      721 gtcagttttt atttttctac taatttggtc gtttatttcg gcgtgtagga catggcaacc
      781 gggcctgaat ttcgcgggta ttctgtttct attccaactt tttcttgatc cgcagccatt
      841 aacgactttt gaatagatac gctgacacgc caagcctcgc tagtcaaaag tgtaccaaac
      901 aacgctttac agcaagaacg gaatgcgcgt gacgctcgcg gtgacgccat ttcgcctttt
      961 cagaaatgga taaatagcct tgcttcctat tatatcttcc caaattacca atacattaca
     1021 ctagcatctg aatttcataa ccaatctcga tacaccaaat cgaatcgatg agcccagaac
     1081 gacgcccggc cgacatccgc cgtgccaccg aggcggacat gccggcggtc tgcaccatcg
     1141 tcaaccacta catcgagaca agcacggtca acttccgtac cgagccgcag gaaccgcagg
     1201 agtggacgga cgacctcgtc cgtctgcggg agcgctatcc ctggctcgtc gccgaggtgg
     1261 acggcgaggt cgccggcatc gcctacgcgg gcccctggaa ggcacgcaac gcctacgact
     1321 ggacggccga gtcgaccgtg tacgtctccc cccgccacca gcggacggga ctgggctcca
     1381 cgctctacac ccacctgctg aagtccctgg aggcacaggg cttcaagagc gtggtcgctg
     1441 tcatcgggct gcccaacgac ccgagcgtgc gcatgcacga ggcgctcgga tatgcccccc
     1501 gcggcatgct gcgggcggcc ggcttcaagc acgggaactg gcatgacgtg ggtttctggc
     1561 agctggactt cagcctgccg gtaccgcccc gtccggtcct gcccgtcacc gagatttgac
     1621 tgcagaatct tggactccca tgttggcaaa ggcaaccaaa caaacaatga atgatccgct
     1681 cctgcatatg gggcggtttg agtatttcaa ctgccatttg ggctgaattg aagacatgct
     1741 cctgtcagaa attccgtgat cttactcaat attcagtaat ctcggccaat atcctaaatg
     1801 tgcgtggctt tatctgtctt tgtattgttt catcaattca tgtaacgttt gcttttctta
     1861 tgaattttca aataaattat cagatccgag ctcgaattaa ttcggcgtta attcagtaca
     1921 ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt caatttgttt acaccacaat
     1981 atatcctgcc accagccagc caacagctcc ccgaccggca gctcggcaca aaatcaccac
     2041 tcgatacagg cagcccatca gtccgggacg gcgtcagcgg gagagccgtt gtaaggcggc
     2101 agactttgct catgttaccg atgctattcg gaagaacggc aactaagctg ccgggtttga
     2161 aacacggatg atctcgcgga gggtagcatg ttgattgtaa cgatgacaga gcgttgctgc
     2221 ctgtgatcac cgcggtttca aaatcggctc cgtcgatact atgttatacg ccaactttga
     2281 aaacaacttt gaaaaagctg ttttctggta tttaaggttt tagaatgcaa ggaacagtga
     2341 attggagttc gtcttgttat aattagcttc ttggggtatc tttaaatact gtagaaaaga
     2401 ggaaggaaat aataaatggc taaaatgaga atatcaccgg aattgaaaaa actgatcgaa
     2461 aaataccgct gcgtaaaaga tacggaagga atgtctcctg ctaaggtata taagctggtg
     2521 ggagaaaatg aaaacctata tttaaaaatg acggacagcc ggtataaagg gaccacctat
     2581 gatgtggaac gggaaaagga catgatgcta tggctggaag gaaagctgcc tgttccaaag
     2641 gtcctgcact ttgaacggca tgatggctgg agcaatctgc tcatgagtga ggccgatggc
     2701 gtcctttgct cggaagagta tgaagatgaa caaagccctg aaaagattat cgagctgtat
     2761 gcggagtgca tcaggctctt tcactccatc gacatatcgg attgtcccta tacgaatagc
     2821 ttagacagcc gcttagccga attggattac ttactgaata acgatctggc cgatgtggat
     2881 tgcgaaaact gggaagaaga cactccattt aaagatccgc gcgagctgta tgatttttta
     2941 aagacggaaa agcccgaaga ggaacttgtc ttttcccacg gcgacctggg agacagcaac
     3001 atctttgtga aagatggcaa agtaagtggc tttattgatc ttgggagaag cggcagggcg
     3061 gacaagtggt atgacattgc cttctgcgtc cggtcgatca gggaggatat cggggaagaa
     3121 cagtatgtcg agctattttt tgacttactg gggatcaagc ctgattggga gaaaataaaa
     3181 tattatattt tactggatga attgttttag tacctagaat gcatgaccaa aatcccttaa
     3241 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     3301 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     3361 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     3421 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     3481 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     3541 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     3601 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     3661 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     3721 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     3781 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     3841 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     3901 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     3961 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     4021 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg
     4081 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca
     4141 atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg
     4201 tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     4261 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     4321 tttcaccgtc atcaccgaaa cgcgcgaggc agggtgcctt gatgtgggcg ccggcggtcg
     4381 agtggcgacg gcgcggcttg tccgcgccct ggtagattgc ctggccgtag gccagccatt
     4441 tttgagcggc cagcggccgc gataggccga cgcgaagcgg cggggcgtag ggagcgcagc
     4501 gaccgaaggg taggcgcttt ttgcagctct tcggctgtgc gctggccaga cagttatgca
     4561 caggccaggc gggttttaag agttttaata agttttaaag agttttaggc ggaaaaatcg
     4621 ccttttttct cttttatatc agtcacttac atgtgtgacc ggttcccaat gtacggcttt
     4681 gggttcccaa tgtacgggtt ccggttccca atgtacggct ttgggttccc aatgtacgtg
     4741 ctatccacag gaaagagacc ttttcgacct ttttcccctg ctagggcaat ttgccctagc
     4801 atctgctccg tacattagga accggcggat gcttcgccct cgatcaggtt gcggtagcgc
     4861 atgactagga tcgggccagc ctgccccgcc tcctccttca aatcgtactc cggcaggtca
     4921 tttgacccga tcagcttgcg cacggtgaaa cagaacttct tgaactctcc ggcgctgcca
     4981 ctgcgttcgt agatcgtctt gaacaaccat ctggcttctg ccttgcctgc ggcgcggcgt
     5041 gccaggcggt agagaaaacg gccgatgccg ggatcgatca aaaagtaatc ggggtgaacc
     5101 gtcagcacgt ccgggttctt gccttctgtg atctcgcggt acatccaatc agctagctcg
     5161 atctcgatgt actccggccg cccggtttcg ctctttacga tcttgtagcg gctaatcaag
     5221 gcttcaccct cggataccgt caccaggcgg ccgttcttgg ccttcttcgt acgctgcatg
     5281 gcaacgtgcg tggtgtttaa ccgaatgcag gtttctacca ggtcgtcttt ctgctttccg
     5341 ccatcggctc gccggcagaa cttgagtacg tccgcaacgt gtggacggaa cacgcggccg
     5401 ggcttgtctc ccttcccttc ccggtatcgg ttcatggatt cggttagatg ggaaaccgcc
     5461 atcagtacca ggtcgtaatc ccacacactg gccatgccgg ccggccctgc ggaaacctct
     5521 acgtgcccgt ctggaagctc gtagcggatc acctcgccag ctcgtcggtc acgcttcgac
     5581 agacggaaaa cggccacgtc catgatgctg cgactatcgc gggtgcccac gtcatagagc
     5641 atcggaacga aaaaatctgg ttgctcgtcg cccttgggcg gcttcctaat cgacggcgca
     5701 ccggctgccg gcggttgccg ggattctttg cggattcgat cagcggccgc ttgccacgat
     5761 tcaccggggc gtgcttctgc ctcgatgcgt tgccgctggg cggcctgcgc ggccttcaac
     5821 ttctccacca ggtcatcacc cagcgccgcg ccgatttgta ccgggccgga tggtttgcga
     5881 ccgctcacgc cgattcctcg ggcttggggg ttccagtgcc attgcagggc cggcaggcaa
     5941 cccagccgct tacgcctggc caaccgcccg ttcctccaca catggggcat tccacggcgt
     6001 cggtgcctgg ttgttcttga ttttccatgc cgcctccttt agccgctaaa attcatctac
     6061 tcatttattc atttgctcat ttactctggt agctgcgcga tgtattcaga tagcagctcg
     6121 gtaatggtct tgccttggcg taccgcgtac atcttcagct tggtgtgatc ctccgccggc
     6181 aactgaaagt tgacccgctt catggctggc gtgtctgcca ggctggccaa cgttgcagcc
     6241 ttgctgctgc gtgcgctcgg acggccggca cttagcgtgt ttgtgctttt gctcattttc
     6301 tctttacctc attaactcaa atgagttttg atttaatttc agcggccagc gcctggacct
     6361 cgcgggcagc gtcgccctcg ggttctgatt caagaacggt tgtgccggcg gcggcagtgc
     6421 ctgggtagct cacgcgctgc gtgatacggg actcaagaat gggcagctcg tacccggcca
     6481 gcgcctcggc aacctcaccg ccgatgcgcg tgcctttgat cgcccgcgac acgacaaagg
     6541 ccgcttgtag ccttccatcc gtgacctcaa tgcgctgctt aaccagctcc accaggtcgg
     6601 cggtggccca tatgtcgtaa gggcttggct gcaccggaat cagcacgaag tcggctgcct
     6661 tgatcgcgga cacagccaag tccgccgcct ggggcgctcc gtcgatcact acgaagtcgc
     6721 gccggccgat ggccttcacg tcgcggtcaa tcgtcgggcg gtcgatgccg acaacggtta
     6781 gcggttgatc ttcccgcacg gccgcccaat cgcgggcact gccctgggga tcggaatcga
     6841 ctaacagaac atcggccccg gcgagttgca gggcgcgggc tagatgggtt gcgatggtcg
     6901 tcttgcctga cccgcctttc tggttaagta cagcgataac cttcatgcgt tccccttgcg
     6961 tatttgttta tttactcatc gcatcatata cgcagcgacc gcatgacgca agctgtttta
     7021 ctcaaataca catcaccttt ttagacggcg gcgctcggtt tcttcagcgg ccaagctggc
     7081 cggccaggcc gccagcttgg catcagacaa accggccagg atttcatgca gccgcacggt
     7141 tgagacgtgc gcgggcggct cgaacacgta cccggccgcg atcatctccg cctcgatctc
     7201 ttcggtaatg aaaaacggtt cgtcctggcc gtcctggtgc ggtttcatgc ttgttcctct
     7261 tggcgttcat tctcggcggc cgccagggcg tcggcctcgg tcaatgcgtc ctcacggaag
     7321 gcaccgcgcc gcctggcctc ggtgggcgtc acttcctcgc tgcgctcaag tgcgcggtac
     7381 agggtcgagc gatgcacgcc aagcagtgca gccgcctctt tcacggtgcg gccttcctgg
     7441 tcgatcagct cgcgggcgtg cgcgatctgt gccggggtga gggtagggcg ggggccaaac
     7501 ttcacgcctc gggccttggc ggcctcgcgc ccgctccggg tgcggtcgat gattagggaa
     7561 cgctcgaact cggcaatgcc ggcgaacacg gtcaacacca tgcggccggc cggcgtggtg
     7621 gtgtcggccc acggctctgc caggctacgc aggcccgcgc cggcctcctg gatgcgctcg
     7681 gcaatgtcca gtaggtcgcg ggtgctgcgg gccaggcggt ctagcctggt cactgtcaca
     7741 acgtcgccag ggcgtaggtg gtcaagcatc ctggccagct ccgggcggtc gcgcctggtg
     7801 ccggtgatct tctcggaaaa cagcttggtg cagccggccg cgtgcagttc ggcccgttgg
     7861 ttggtcaagt cctggtcgtc ggtgctgacg cgggcatagc ccagcaggcc agcggcggcg
     7921 ctcttgttca tggcgtaatg tctccggttc tagtcgcaag tattctactt tatgcgacta
     7981 aaacacgcga caagaaaacg ccaggaaaag ggcagggcgg cagcctgtcg cgtaacttag
     8041 gacttgtgcg acatgtcgtt ttcagaagac ggctgcactg aacgtcagaa gccgactgca
     8101 ctatagcagc ggaggggttg gatcaaagta ctttgatccc gaggggaacc ctgtggttgg
     8161 catgcacata caaatggacg aacggataaa ccttttcacg cccttttaaa tatccgatta
     8221 ttctaataaa cgctcttttc tcttaggttt acccgccaat atatcctgtc aaacactgat
     8281 agtttaaact gaaggcggga aacgacaatc tgatccaagc tcaagctgct ctagcattcg
     8341 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
     8401 cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc
     8461 cagtcacgac gttgtaaaac gacggccagt gccaagcttc cttttgtctc ccttttgtct
     8521 cccttttgtc tcccttttgt ctcccttttg tctccctttt gtctcccttt tgtctctcgc
     8581 aagacccttc ctckmacttc atttggggcc cacatacacc aaaaaaatgc tgcataattc
     8641 tcggggcagc aagtcggtta cccggccgcc gtgctggacc gggttgaatg gtgcccgtaa
     8701 ctttcggtag agcggacggc caatactcaa cttcaaggaa tctcacccat gcgcgccggc
     8761 ggggaaccgg agttcccttc agtgagcgtt attagttcgc cgctcggtgt gtcgtagata
     8821 ctagcccctg gggcactttt gaaatttgaa taagatttat gtaatcagtc ttttaggttt
     8881 gaccggttct gccgcttttt ttaaaattgg atttgtaata ataaaacgca attgtttgtt
     8941 attgtggcgc tctatcatag atgtcgctat aaacctattc agcacaatat attgttttca
     9001 ttttaatatt gtacatataa gtagtagggt acaatcagta aattgaacgg agaatattat
     9061 tcataaaaat acgatagtaa cgggtgatat attcattaga atgaaccgaa accggcggta
     9121 aggatctgag ctacacatgc tcaggttttt tacaacgtgc acaacagaat tgaaagcaaa
     9181 tatcatgcga tcataggcgt ctcgcatatc tcattaaagc aggactctag ggactagtcc
     9241 cgggtcttaa ttaagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     9301 gcgcacgagg gagcttccag gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     9361 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     9421 aaaacgccag caacgcggat cacagcaaaa agcccgctca ttaggcgggc taaattctca
     9481 tgtttgacag cttatcatcg ataagtttta atgagttatc gagattttca ggagctaagg
     9541 aagctaaaat ggagaaagaa atcactggat ataccaccgt tgatatatcc caatggcatc
     9601 gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg tacctataac cagaccgttc
     9661 agctggatat tacggccttt ttaaagaccg taaagaaaaa taagcacaag ttttatccgg
     9721 cctttattca cattcttgcc cgcctgatga atgctcatcc ggagttccgt atggcaatga
     9781 aagacggtga gctggtaata tgggatagtg ttcacccttg ttacaccgtt ttccatgagc
     9841 aaactgaaac gttttcatcg ctctggagtg aataccacga cgatttccgg cagtttctac
     9901 acatatattc gcaagatgtg gcgtgttacg gtgaaaacct ggcctatttc cctaaagggt
     9961 ttattgagaa tatgtttttc gtctcagcca atccctgggt gagtttcacc agttttgatt
    10021 taaacgtggc caatatggac aacttcttcg cccccgtttt caccatgggc aaatattata
    10081 cgcaaggcga caaggtgctg atgccgctgg cgattcaggt tcatcatgcc gtttgtgatg
    10141 gcttccatgt cggcagaatg cttaatgaat tacaacagta ctgcgatgag tggcagggcg
    10201 gggcgtaatt ggtacaggcc tgtcgacgtt agaacgcggc tacaattaat acataacctt
    10261 atgtatcata cacatacgat ttaggtgaca ctatagaact cgagcagctg aagctccaat
    10321 gtgatggtct agaggtcgca tgcgatcccc gtcaccggtg tgagggaact agttttgatc
    10381 ttgaaagatc ttttatcttt agagttaagg actctttcgt attttggtga ggttttatcc
    10441 tcttgagttt tggtcataga cctattcatg gctctgatac caatttttaa gcgggggctt
    10501 atgcggatta tttcttaaat tgataagggg ttattagggg gtatagggta taaatacaat
    10561 cattccctta gcgtatagta taagtatagt agcgtacctc tatcaaattt ccatcttctt
    10621 accttgcaca gggcctgcaa ccttatcctt ccttgtcttc ctccttcctt ccgtccactt
    10681 catcatattt aaaccaaacc tacgggggag tcaacgtaac caaccctgcc ttagcatctt
    10741 ttccctaacg gcctcctgcc taagcggtac ttctagcttc gaacggcgtc tgggctccag
    10801 gtctagtcgt ctcgtgtctg gtttatattc acgacaaaga tctataggga ctttaggaga
    10861 tctggatttt agtactggat ttcggtttta ggaattagaa attttattga tagaagtatt
    10921 ttacaaatac aaatacatac taagggtctc ttatatgctc aacacatgag cgaaacccta
    10981 taagaaccct aatttccctt atcgggaaac tactcacaca ttatttatgg agaaaataga
    11041 gagagataga tttgtagaga gagactggtg atttcagcgt accgaattca ctagtggatc
    11101 ctggagaagc atacgggcac gtgagattct tcttctttag accatattta aattagacca
    11161 taacaacaac aactggagaa gcatacgggc acgtgagggt accaagcttg ggctgtcctc
    11221 tccaaatgaa atgaacttcc ttatatagag gaagggtctt gcgaaggata gtggggttgt
    11281 gcgtcatccc ttatgtcagt ggagatgtca catcaatcca cttgctttga agacgtggtt
    11341 ggaacgtctt ctttttccac gatgctcctc gtgggtgggg gtccatcttt gggaccactg
    11401 tcggcagagg catcttgaat gatagccttt cctttatcgc gatgatggca tttgtaggag
    11461 ccaccttcct tttctactgt cctttcgatg aggt
//
