LOCUS       sequence-167048-         921 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses PCNA1 variant (G108C) fused to an FAD domain of P450 BM3 
            (A74G/C773S/C810S) in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Tan CY, Hirakawa H, Suzuki R, Haga T, Iwata F, Nagamune T
  TITLE     Immobilization of a Bacterial Cytochrome P450 Monooxygenase System 
            on a Solid Support.
  JOURNAL   Angew Chem Int Ed Engl. 2016 Oct 26. doi: 10.1002/anie.201608033.
  PUBMED    27781345
REFERENCE   2  (bases 1 to 921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Angew Chem 
            Int Ed Engl. 2016 Oct 26. doi: 10.1002/anie.201608033."
COMMENT     Sequence Label: p1G108CFa
FEATURES             Location/Qualifiers
     source          1..921
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             19..41
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             60..77
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             87..104
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ttcccctcta gaaataattt tgtttaactt taagaaggag atataccatg ggcagcagcc
       61 atcatcatca tcatcacagc agcggcctgg tgccgcgcgg cagccatatg tttaaaattg
      121 tgtatccgaa cgccaaagat ttctttagct tcatcaacag cattaccaac gttacggatt
      181 ctatcatcct gaacttcacc gaagatggca tcttcagtcg tcatctgacg gaagataaag
      241 tgctgatggc aatcatgcgc attccgaaag atgttctgtc tgaatacagt attgatagcc
      301 cgacctctgt gaaactggat gtgagctctg tgaagaaaat tctgagcaaa gcaagtagca
      361 aaaaagcgac gattgaactg accgaaacgg attctggcct gaaaatcatc atccgtgatg
      421 aaaaatcttg cgccaaaagt accatctaca tcaaagcaga aaaaggccag gttgaacagc
      481 tgacggaacc gaaagtgaac ctggccgtta attttaccac ggatgaaagc gtgctgaacg
      541 ttatcgcggc cgatgtgacc ctggttggtg aagaaatgcg tatcagcacg gaagaagata
      601 aaatcaaaat tgaagcgggc gaagaaggta aacgctatgt ggcctttctg atgaaagata
      661 aaccgctgaa agaactgagc atcgatacct ctgcctctag tagctacagc gcagaaatgt
      721 tcaaagatgc ggtgaaaggc ctgcgtggtt ttagtgcgcc gacgatggtg tctttcggtg
      781 aaaatctgcc gatgaaaatt gatgtggaag cagttagcgg cggtcacatg atcttctgga
      841 ttgcgccgcg cctgggaggt agcatgccgc ttgcgaaaat gcacggtgcg ttttcaacga
      901 acgtcgtagc aagcaaagaa c
//
