LOCUS       sequence-167051-         606 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses minor satellite-specific sgRNA..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 606)
  AUTHORS   Schmidtmann E, Anton T, Rombaut P, Herzog F, Leonhardt H
  TITLE     Determination of Local Chromatin Composition by CasID.
  JOURNAL   Nucleus. 2016 Sep 27:0.
  PUBMED    27676121
REFERENCE   2  (bases 1 to 606)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleus. 
            2016 Sep 27:0."
COMMENT     Sequence Label: pEX-A-U6-MiSgRNA_PuroR
FEATURES             Location/Qualifiers
     source          1..606
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     223..240
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    357..378
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(393..398,399..416,417..423)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    431..447
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     436..458
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    493..517
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_RNA        complement(529..604)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
       61 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
      121 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
      181 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
      241 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
      301 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
      361 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
      421 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa ttcggatcca agcttgcggc
      481 cgcaggttaa tgccaacttt gtacaagaaa gctgggtcta gaaaaaaagc accgactcgg
      541 tgccactttt tcaagttgat aacggactag ccttatttta acttgctatt tctagctcta
      601 aaacac
//
