LOCUS       sequence-167056-         986 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  rAAV-based template for genome engineering of the p53 C-terminus 
            containing PQS1 and 3xFLAG tags and a selection cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Vandemoortele G, Staes A, Gonnelli G, Samyn N, De Sutter D, 
            Vandermarliere E, Timmerman E, Gevaert K, Martens L, Eyckerman S
  TITLE     An extra dimension in protein tagging by quantifying universal 
            proteotypic peptides using targeted proteomics.
  JOURNAL   Sci Rep. 2016 Jun 6;6:27220. doi: 10.1038/srep27220.
  PUBMED    27264994
REFERENCE   2  (bases 1 to 986)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-06-6"; volume: "6:27220. doi"; pages: " 
            10.1038/srep27220"
COMMENT     Sequence Label: pAav-TP53-PQS1-3xFLAG
FEATURES             Location/Qualifiers
     source          1..986
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     1..24
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     protein_bind    71..104
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(132..197)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    complement(268..301)
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
ORIGIN
        1 cagcggggct gctaaagcgc atgctccaga ctgccttggg aaaagcgcct cccctacccg
       61 gtagggcgcg ataacttcgt ataatgtatg ctatacgaag ttatccgaag ctttcaagcc
      121 tcgggctcgc ccttgtcatc gtcatccttg taatcaatat catgatcttt ataatcaccg
      181 tcatggtctt tgtagtcgtc gtacaggtag gcgaatctgg aggtctccag gatttcgctc
      241 acggcctctc tgaagatcag ggtcagtgaa gttcctatac tatttgaaga ataggaactt
      301 cggaatagga acttcccgaa ttcgctgggc ccgctgtctg agtcaggccc ttctgtcttg
      361 aacatgagtt ttttatggcg ggaggtagac tgaccctttt tggacttcag gtggctgtag
      421 gagacagaag cagggaggag agatgacatc acatgagtga gagggtctgt gccccttttc
      481 cctgaccaat gctttgaagg gcctaaggct gggacaacgg gaattcaaat caagatggtg
      541 gccacacccc atgcaaatat gtttactgag cacctcagag tattagtgtg tattagtctc
      601 gtaatcttcc cttaccccat tttactttat ttatcttttt tgagacggag tttcactctt
      661 gttgcccagg ctggagtgta atggtgagat ctcagctcac cgcaacctct gcctcccggg
      721 ttcaagcgat tctcctgcct cagcctcccg agtaggtagc tgggattaca ggcatgcatc
      781 accacgcccg gctacttttg tatttttagt agagatgggg tttctccatg ttggtcaggc
      841 tgggctcaaa ctcccgacct caggtgatcc actcgccttg gcctcccaga gtgtgggatt
      901 cgtgagccac tgcgcccggc ccccttaccc cattttatat ataaggaaac tgagtttgac
      961 gggggtcacc taggacctgc cggtgc
//
