LOCUS       sequence-167058-        6909 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing ERBB2IP , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6909)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 6909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_ERBB2IP_p.E463K
FEATURES             Location/Qualifiers
     source          1..6909
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(3626..3637)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(4309..4408)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(4425..4444)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4426..4444)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4449..4465)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4449..4465)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             4896..5687
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      5780..6368
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6269..6288
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6522..6539
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(6698..6725)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(6817..6903)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcac catgactaca aaacgaagtt tgtttgtgcg gttggtacca tgtcgctgtc
      241 tacgagggga agaggagact gtcactactc ttgattattc tcattgcagc ttagaacaag
      301 ttccgaaaga gatttttact tttgaaaaaa ccttggagga actctattta gatgctaatc
      361 agattgaaga gcttccaaag caacttttta actgtcagtc tttacacaaa ctgagtttgc
      421 cagacaatga tttaacaacg ttaccagcat ccattgcaaa ccttattaat ctcagggaac
      481 tggatgtcag caagaatgga atacaggagt ttccagaaaa tataaaaaat tgtaaagttt
      541 tgacaattgt ggaggccagt gtaaacccta tttccaagct ccctgatgga ttttctcagc
      601 tgttaaacct aacccagttg tatctgaatg atgcttttct tgagttcttg ccagcaaatt
      661 ttggcagatt aactaaactc caaatattag agcttagaga aaaccagtta aaaatgttgc
      721 ctaaaactat gaatagactg acccagctgg aaagattgga tttgggaagt aacgaattca
      781 cggaagtgcc tgaagtactt gagcaactaa gtggattgaa agagttttgg atggatgcta
      841 atagactgac ttttattcca gggtttattg gtagtttgaa acagctcaca tatttggatg
      901 tttctaaaaa taatattgaa atggttgaag aaggaatttc aacatgtgaa aaccttcaag
      961 acctcctatt atcaagcaat tcacttcagc agcttcctga gcctattggt tcgttgaaga
     1021 atataacaac gcttaaaata gatgaaaacc agttaatgta tctgccagac tctataggag
     1081 ggttaatatc agtagaagaa ctggattgta gtttcaatga agttgaagct ttgccttcat
     1141 ctattgggca gcttactaac ttaagaactt ttgctgctga tcataattac ttacaacagt
     1201 tgcccccaga gattggaagc tggaaaaata taactgtgct gtttctccat tccaataaac
     1261 ttgagacact tccagaggaa atgggtgata tgcaaaaatt aaaagtcatt aatttaagtg
     1321 ataatagatt aaagaattta ccctttagct ttacaaagct acagcaattg acagctatgt
     1381 ggctctcaga taatcagtcc aaacccctga tacctcttca aaaagaaact gattcagaga
     1441 cccagaaaat ggtgcttacc aactacatgt tccctcaaca gccaaggact gaggatgtta
     1501 tgtttatatc agataatgaa agttttaacc cttcattgtg ggaggaacag aggaaacagc
     1561 gggctcaagt tgcatttaaa tgtgatgaag acaaagatga aagggaggca cctcccaggg
     1621 agggaaattt aaaaagatat ccaacaccat acccagatga gcttaagaat atggtcaaaa
     1681 ctgttcaaac cattgtacat agattaaaag atgaagagac caatgaagac tcaggaagag
     1741 atttgaaacc acatgaagat caacaagata taaataaaga tgtgggtgtg aagacctcgg
     1801 aaagtactac tacagtaaaa agcaaagttg gtgaaagaga aaaatatatg ataggaaact
     1861 ctgtacagaa gatcagtgaa cctgaagctg agattagtcc tgggagttta ccagtgactg
     1921 caaatatgaa agcctctgag aacttgaagc atattgttaa ccatgatgat gtttttgagg
     1981 aatctgaaga actttcttct gatgaagaga tgaaaatggc ggagatgcga ccaccattaa
     2041 ttgaaacctc tattaaccag ccaaaagtcg tagcacttag taataacaaa aaagatgata
     2101 caaaggaaac agattcttta tcagatgaag ttacacacaa tagcaatcag aataacagca
     2161 attgttcttc tccatctcgg atgtctgatt cagtttctct taatactgat agtagtcaag
     2221 acacctcact ctgctctcca gtgaaacaaa ctcatattga tattaattcc aaaatcaggc
     2281 aagaagatga aaattttaac agccttttac aaaatggaga tattttaaac agttcaacag
     2341 aggaaaagtt caaagctcat gataaaaaag attttaactt acctgaatat gatttgaatg
     2401 ttgaagagcg attagttcta attgagaaaa gtgttgactc aacagccaca gctgatgaca
     2461 ctcacaaatt agatcatatc aatatgaatc ttaataaact tataactaat gatacattcc
     2521 aaccagagat catggaaaga tcaaaaacac aggatattgt gcttggaaca agctttttaa
     2581 gcattaattc taaagaggaa actgagcact tggaaaatgg aaacaagtat cctaatttgg
     2641 aatccgtaaa taaggtaaat ggacattctg aggaaacttc ccagtctcct aataggactg
     2701 aaccacatga cagtgattgt tctgttgact taggtatttc caaaagcact gaagatctct
     2761 cccctcagaa aagtggtcca gttggatctg ttgtgaaatc tcatagcata actaatatgg
     2821 agattggagg gctaaaaatc tatgatattc ttagtgataa tggacctcag cagccaagta
     2881 caaccgttaa aatcacatct gctgttgatg gaaaaaatat agtcaggagc aagtctgcca
     2941 cactgttgta tgatcaacca ttgcaggtat ttactggttc ttcctcatct tctgatttaa
     3001 tatcaggaac aaaggcaatt ttcaagtttg attcaaatca taatcccgaa gagccaaata
     3061 taataagagg ccccacaagt ggcccacaat ctgcacctca aatatatggt cctccacagt
     3121 ataatatcca atacagtagc agtgctgcag tcaaagacac tttgtggcac tccaaacaaa
     3181 atccccaaat agaccatgcc agttttcctc ctcagctcct tcctagatca gagagcacag
     3241 aaaatcaaag ttatgctaaa cattctgcca atatgaattt ctctaatcat aacaatgttc
     3301 gagctaatac tgcataccat ttacatcaga gacttggccc agcaagacat ggggaaatgt
     3361 gggccatctc accaaacgac cgacttattc ctgcagtaac tcgaagtaca atccagcgac
     3421 aaagtagtgt gtcctccaca gcctctgtaa atcttggtga tccaggctct acaaggcggg
     3481 ctcagattcc tgaaggagat tatttatcat acagagagtt ccactcagcg ggaagaactc
     3541 ctccaatgat gccaggatca cagagacccc tttctgcacg aacatacagc atagatggtc
     3601 caaatgcatc aagacctcag agtgctcgac cctctattaa tgaaatacca gagagaacta
     3661 tgtcagttag tgatttcaat tattcacgga ctagtccttc aaaaagacca aatgcaaggg
     3721 ttggttctga gcattcttta ttagatcctc caggaaaaag taaagttcct cgtgactgga
     3781 gagaacaagt acttcgacat attggagcca aaaagttaga aaagatgcct ttgagtaatg
     3841 gacagatggg ccagcctctc aggcctccgg caaattatag tcaaatacat cacccccctc
     3901 aggcatctgt ggcaaggcat ccctctagag aacaactaat tgattacttg atgctgaaag
     3961 tggcccacca gcctccatat acacagcccc attgttctcc tagacaaggc catgaactgg
     4021 caaaacaaga gattcgagtg agggttgaaa gggatccaga acttggattt agcatatcag
     4081 gtggtgtcgg gggtagagga aacccattca gacctgatga tgatggtata tttgtaacaa
     4141 gggtacaacc tgaaggacca gcatcaaaat tactgcagcc aggtgataaa attatccagg
     4201 ctaatggcta cagttttata aatattgaac atggacaagc agtgtccttg ctaaaaactt
     4261 tccagaatac agttgaactc atcattgtac gagaagtttc ctcatgattg ccaactttct
     4321 tgtacaaagt tggcattata agaaagcatt gcttatcaat ttgttgcaac gaacaggtca
     4381 ctatcagtca aaataaaatc attatttgcc atccagctga tatcccctat agtgagtcgt
     4441 attacatggt catagctgtt tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt
     4501 acattgcaca agataaaaat atatcatcat gcctcctcta gaccagccag gacagaaatg
     4561 cctcgacttc gctgctaccc aaggttgccg ggtgacgcac accgtggaaa cggatgaagg
     4621 cacgaaccca gtggacataa gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc
     4681 gctcacgcaa ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg
     4741 ttttcatggc ttgttatgac tgtttttttg gggtacagtc tatgcctcgg gcatccaagc
     4801 agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc
     4861 agcagggcag tcgccctaaa acaaagttaa acattatgag ggaagcggtg atcgccgaag
     4921 tatcgactca actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc
     4981 tggccgtaca tttgtacggc tccgcagtgg atggcggcct gaagccacac agtgatattg
     5041 atttgctggt tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg
     5101 accttttgga aacttcggct tcccctggag agagcgagat tctccgcgct gtagaagtca
     5161 ccattgttgt gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat
     5221 ttggagaatg gcagcgcaat gacattcttg caggtatctt cgagccagcc acgatcgaca
     5281 ttgatctggc tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag
     5341 cggcggagga actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa
     5401 ccttaacgct atggaactcg ccgcccgact gggctggcga tgagcgaaat gtagtgctta
     5461 cgttgtcccg catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg
     5521 ccgactgggc aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctagac
     5581 aggcttatct tggacaagaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat
     5641 ttgtccacta cgtgaaaggc gagatcacca aggtagtcgg caaataaccc tcgagccacc
     5701 catgaccaaa atcccttaac gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag
     5761 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     5821 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     5881 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     5941 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     6001 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     6061 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     6121 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     6181 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     6241 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     6301 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     6361 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     6421 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     6481 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     6541 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     6601 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata
     6661 cgcgtaccgc tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc
     6721 cttctgctta gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg
     6781 ccgttgcttc acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt
     6841 caccgacaaa caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat
     6901 ttgatgcct
//
