LOCUS       sequence-167059-         924 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  rAAV-based template for genome engineering of the p53 C-terminus 
            containing PQS1 and 3xFLAG tags and a selection cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 924)
  AUTHORS   Vandemoortele G, Staes A, Gonnelli G, Samyn N, De Sutter D, 
            Vandermarliere E, Timmerman E, Gevaert K, Martens L, Eyckerman S
  TITLE     An extra dimension in protein tagging by quantifying universal 
            proteotypic peptides using targeted proteomics.
  JOURNAL   Sci Rep. 2016 Jun 6;6:27220. doi: 10.1038/srep27220.
  PUBMED    27264994
REFERENCE   2  (bases 1 to 924)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-06-6"; volume: "6:27220. doi"; pages: " 
            10.1038/srep27220"
COMMENT     Sequence Label: pAav-TP53-PQS1-3xFLAG
FEATURES             Location/Qualifiers
     source          1..924
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(121..138)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    127..351
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    complement(376..409)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
       61 tctatcgcct tcttgacgag ttcttctgag gggatcaatt ctctagagct cgctgatcag
      121 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
      181 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
      241 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
      301 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg
      361 cggaaagaac cagctataac ttcgtatagc atacattata cgaagttata gtaatcgatt
      421 gacattctcc acttcttgtt ccccactgac agcctcccac ccccatctct ccctcccctg
      481 ccattttggg ttttgggtct ttgaaccctt gcttgcaata ggtgtgcgtc agaagcaccc
      541 aggacttcca tttgctttgt cccggggctc cactgaacaa gttggcctgc actggtgttt
      601 tgttgtgggg aggaggatgg ggagtaggac ataccagctt agattttaag gtttttactg
      661 tgagggatgt ttgggagatg taagaaatgt tcttgcagtt aagggttagt ttacaatcag
      721 ccacattcta ggtaggggcc cacttcaccg tactaaccag ggaagctgtc cctcactgtt
      781 gaattttctc taacttcaag gcccatatct gtgaaatgct ggcatttgca cctacctcac
      841 agagtgcatt gtgagggtta atgaaataat gtacatctgg ccttgaaacc accttttatt
      901 acatggggtc tagaacttga cccc
//
