LOCUS       sequence-16706-e        1338 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1338)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1997
REFERENCE   2  (bases 1 to 1338)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: L3604
FEATURES             Location/Qualifiers
     source          1..1338
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             475..495
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(538..566)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 gnnttnactg atcttctaac taactattct catttaaatt ttcagagctt aaaaatggct
       61 gaaatcactc acaacgatgg atacgctaac aacttggaaa tgaaataagc ttgcatgcct
      121 gcaggtcgac tctagaggat ccccaattct tgagacttcc actatcttct ctggcaccct
      181 ccactacgcg taggcgtctc tcgcttcgtg tattcccggg aagccgggtt cccgtctctc
      241 ccgccgctgc cgctgccgca cacagcttta cacctcgtag aatccccaaa gaggggcgtg
      301 gcttgcgggt gccaacatcc tcctgccgag gaagaagcag gcactcatca ctcgcatcat
      361 caacctcggg attggccaaa ggacccaaag gtatgtttcg aatgatacta acataacata
      421 gaacattttc aggaggaccc ttggagggta ccgagctcag aaaaaatgac tgctccaaag
      481 aagaagcgta aggtaccggt agaaaaaatg agtaaaggag aagaactttt cactggagtt
      541 gtcccaattc ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtcagtgga
      601 gagggtgaag gtgatgcaac atacggaaaa cttaccctta aatttatttg cactactgga
      661 aaactacctg ttccatgggt aagtttaaac atatatatac taactaaccc tgattattta
      721 aattttcagc caacacttgt cactactttc acttatggng ttcaatgctt ctcgagatac
      781 ccagatcata tgaaacagca tgactttttc aagagtgcca tgcccgaagg ttatgtacag
      841 gaaagaacta tatttttcaa agatgacggg gaactacaag acacgtaagt ttaaacagtt
      901 cggtactaac taaccataca tatttaaaat tttcaggtgc tgaagtcaag tttggaaggt
      961 gatacccctt gttaatagaa tccgagttaa aaggtattga ttttaaagaa gatggaaaca
     1021 ttctgggacc caattggaat acactatatt tcncacaacg tatacntccc gcaaanaaca
     1081 aagatgggat caagttgtaa gttaanctga tttnctanca actaatcgat taaatttcnn
     1141 anctcaantn gaccnnctng anangnaggn tncnctagcn acntttcaca aancccattg
     1201 ggangccntt ctttnccaaa ncatnntgtc cnaaattgcc tttnaaaatc caaaaaaana
     1261 acccggncct ntngatnaaa ccggnggana cnggggggga antanaaacc tncgaatggg
     1321 ttaanntccn nccacccc
//
