LOCUS       sequence-167067-         883 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  rAAV-based template for genome engineering of protein C-termini 
            containing PQS1 and 3xFLAG tags and a selection cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Vandemoortele G, Staes A, Gonnelli G, Samyn N, De Sutter D, 
            Vandermarliere E, Timmerman E, Gevaert K, Martens L, Eyckerman S
  TITLE     An extra dimension in protein tagging by quantifying universal 
            proteotypic peptides using targeted proteomics.
  JOURNAL   Sci Rep. 2016 Jun 6;6:27220. doi: 10.1038/srep27220.
  PUBMED    27264994
REFERENCE   2  (bases 1 to 883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-06-6"; volume: "6:27220. doi"; pages: " 
            10.1038/srep27220"
COMMENT     Sequence Label: pAav-MCS-PQS1-3xFLAG
FEATURES             Location/Qualifiers
     source          1..883
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(65..565)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(113..132)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     507..530
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     protein_bind    577..610
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(638..703)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    complement(774..807)
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
ORIGIN
        1 agcggccgga gaacctgcgt gcaatccatc ttgttcaatg gccgatccca tattggctgc
       61 aggtcgaaag gcccggagat gaggaagagg agaacagcgc ggcagacgtg cgcttttgaa
      121 gcgtgcagaa tgccgggctt ccggaggacc ttcgggcgcc cgccccgccc ctgagcccgc
      181 ccctgagccc gcccccggac ccaccccttc ccagcctctg agcccagaaa gcgaaggagc
      241 caaagctgct attggccgct gccccaaagg cctacccgct tccattgctc agcggtgctg
      301 tccatctgca cgagactagt gagacgtgct acttccattt gtcacgtcct gcacgacgcg
      361 agctgcgggg cgggggggaa cttcctgact aggggaggag tagaaggtgg cgcgaagggg
      421 ccaccaaaga acggagccgg ttggcgccta ccggtggatg tggaatgtgt gcgaggccag
      481 aggccacttg tgtagcgcca agtgcccagc ggggctgcta aagcgcatgc tccagactgc
      541 cttgggaaaa gcgcctcccc tacccggtag ggcgcgataa cttcgtataa tgtatgctat
      601 acgaagttat ccgaagcttt caagcctcgg gctcgccctt gtcatcgtca tccttgtaat
      661 caatatcatg atctttataa tcaccgtcat ggtctttgta gtcgtcgtac aggtaggcga
      721 atctggaggt ctccaggatt tcgctcacgg cctctctgaa gatcagggtc agtgaagttc
      781 ctatactatt tgaagaatag gaacttcgga ataggaactt cccgaattcg ctgggccctt
      841 tacgcgtttg cggccgcagg aacccctagt gatggagttg gcc
//
