LOCUS       sequence-16707-e        1304 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1304)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1997
REFERENCE   2  (bases 1 to 1304)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: L3605
FEATURES             Location/Qualifiers
     source          1..1304
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             477..497
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(540..568)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 gnnnttacat gatcttacta actaactatt ctcatttaaa ttttcagagc ttaaaaatgg
       61 ctgaaatcac tcacaacgat ggatacgcta acaacttgga aatgaaataa gcttgcatgc
      121 ctgcaggtcg actctagagg atccccaatt cttgagactt ccactatctt ctctggcacc
      181 ctccactacg cgtaggcgtc tctcgcttcg tgtattcccg ggaagccggg ttcccgtctc
      241 tcccgccgct gccgctgccg cacacagctt tacacctcgt agaatcccca aagaggggcg
      301 tggcttgcgg gtgccaacat cctcctgccg aggaagaagc aggcactcat cactcgcatc
      361 atcaacctcg ggattggcca aaggacccaa aggtatgttt cgaatgatac taacataaca
      421 tagaacattt tcaggaggac ccttggaggg taccgagctc agaaaaaatg actgctccaa
      481 agaagaagcg taaggtaccg gtagaaaaaa tgagtaaagg agaagaactt ttcactggag
      541 ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg gcacaaattt tctgtcagtg
      601 gagagggtga aggtgatgca acatacggaa aacttaccct taaatttatt tgcactactg
      661 gaaaactacc tgttccatgg gtaagtttaa acatatatat actaactaac cctgattatt
      721 taaattttca gccaacactt gtcactactt tcacttatgg tgttcaatgc ttctcgagat
      781 acccagatca tatgaaacag catgactttt tcaagagtgc catgcccgaa ggttatgtac
      841 aggaaagaac tatatttttc aaagatgacg ggaactacaa gacacgtaag tttaaacagt
      901 tcggtactaa ctaaccatac atatttaaat tttcaggtgc tgaantcnag tttgaagggg
      961 ganncccttg gtnaataaan ncgagtttaa anggtttttn tttttnaaan aaaangggaa
     1021 actttcttgg aacacnaaat tggaantaca actntttttc ccacaacgnt atacntccct
     1081 ggcnaanaaa naaaaaanng gnatcaaagg gggtaagttt aaacntgant tttacnaacn
     1141 aacnaaatcg nanttnaant ttnccnaann tncaaaatta aacccnacnt tngananggg
     1201 gagnntttcc cntgcnaacn ttttccnaaa aattcccctt ggggagggcc tgttcttttn
     1261 caaaaaanan tnntnttcca aanttgncct ttnaaaaanc aaaa
//
