LOCUS       sequence-167071-         901 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  rAAV-based template for genome engineering of the IQGAP1 C-terminus 
            containing PQS1 and 3xFLAG tags and a selection cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Vandemoortele G, Staes A, Gonnelli G, Samyn N, De Sutter D, 
            Vandermarliere E, Timmerman E, Gevaert K, Martens L, Eyckerman S
  TITLE     An extra dimension in protein tagging by quantifying universal 
            proteotypic peptides using targeted proteomics.
  JOURNAL   Sci Rep. 2016 Jun 6;6:27220. doi: 10.1038/srep27220.
  PUBMED    27264994
REFERENCE   2  (bases 1 to 901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-06-6"; volume: "6:27220. doi"; pages: " 
            10.1038/srep27220"
COMMENT     Sequence Label: pAav-IQGAP1-PQS1-3xFLAG
FEATURES             Location/Qualifiers
     source          1..901
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(121..138)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    127..351
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    complement(376..409)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
       61 tctatcgcct tcttgacgag ttcttctgag gggatcaatt ctctagagct cgctgatcag
      121 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
      181 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
      241 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
      301 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg
      361 cggaaagaac cagctataac ttcgtatagc atacattata cgaagttata gtaatcgata
      421 actcgagtaa ttgatcgttt gctgccagcc cagaaggatg aaggaaagaa gcacctcaca
      481 gctcctttct aggtccttct ttcctcattg gaagcaaaga cctagccaac aacagcacct
      541 caatctgata cactcccgat gccacatttt taactcctct cgctctgatg ggacatttgt
      601 tacccttttt tcatagtgaa attgtgtttc aggcttagtc tgacctttct ggtttcttca
      661 ttttcttcca ttacttagga aagagtggaa actccactaa aatttctctg tgttgttaca
      721 gtcttagagg ttgcagtact atattgtaag ctttggtgtt tgtttaatta gcaataggga
      781 tggtaggatt caaatgtgtg tcatttagaa gtggaagcta ttagcaccaa tgacataaat
      841 acatacaaga cacacaacta aaatgtcatg ttattaacag ttattaggtt gtcatttaaa
      901 a
//
