LOCUS       sequence-167072-         978 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  rAAV-based template for genome engineering of the IQGAP1 C-terminus 
            containing PQS1 and 3xFLAG tags and a selection cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Vandemoortele G, Staes A, Gonnelli G, Samyn N, De Sutter D, 
            Vandermarliere E, Timmerman E, Gevaert K, Martens L, Eyckerman S
  TITLE     An extra dimension in protein tagging by quantifying universal 
            proteotypic peptides using targeted proteomics.
  JOURNAL   Sci Rep. 2016 Jun 6;6:27220. doi: 10.1038/srep27220.
  PUBMED    27264994
REFERENCE   2  (bases 1 to 978)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-06-6"; volume: "6:27220. doi"; pages: " 
            10.1038/srep27220"
COMMENT     Sequence Label: pAav-IQGAP1-PQS1-3xFLAG
FEATURES             Location/Qualifiers
     source          1..978
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     1..24
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     protein_bind    71..104
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(132..197)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    complement(268..301)
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
ORIGIN
        1 cagcggggct gctaaagcgc atgctccaga ctgccttggg aaaagcgcct cccctacccg
       61 gtagggcgcg ataacttcgt ataatgtatg ctatacgaag ttatccgaag ctttcaagcc
      121 tcgggctcgc ccttgtcatc gtcatccttg taatcaatat catgatcttt ataatcaccg
      181 tcatggtctt tgtagtcgtc gtacaggtag gcgaatctgg aggtctccag gatttcgctc
      241 acggcctctc tgaagatcag ggtcagtgaa gttcctatac tatttgaaga ataggaactt
      301 cggaatagga acttcccgaa ttccttcccg tagaactttt tgttgagaag gaagatcagg
      361 aggttgacat ttactttagc tctatcaaat aatttcatga ctgcaactcc ttcatactgt
      421 agctgcagca ggtcctatta acaaaacaaa acaaaacaaa acaaaacatt ttgacagtta
      481 atcaagtctg tggaacaagg aaaaaggaca caatgtaggg agggatccag atggactgag
      541 gcctgccttg tgtgccagat gtgaactggg gcgaaagaaa agttccacgc taggcaacta
      601 cccactgcac aaccagcctg agagctggag caatagggca cattggccaa gggaggcttc
      661 tggttgattt ggagttagga agtcatgttg ttcatatcaa aattcatcat acatggaaag
      721 aaaaagaaac atgttcaatt gccacacaca tctaagcttt ttatttcaga agtcacaatg
      781 acattttccc atttcatcca tgttatgggt cactagtttt aataaatctg gaaaaggtga
      841 agcatttaaa cctatgatga aatatactaa gcacggggaa atgagataag gctagtaatg
      901 tcaccttaat actatctgat agattcttaa tttctattcc atatgagaac actttctatc
      961 ccgaaggcaa ggggaaaa
//
