LOCUS       sequence-167074-         977 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  rAAV-based template for genome engineering of the MDM2 protein 
            C-terminus containing PQS2 and 3xHA tags and a selection cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Vandemoortele G, Staes A, Gonnelli G, Samyn N, De Sutter D, 
            Vandermarliere E, Timmerman E, Gevaert K, Martens L, Eyckerman S
  TITLE     An extra dimension in protein tagging by quantifying universal 
            proteotypic peptides using targeted proteomics.
  JOURNAL   Sci Rep. 2016 Jun 6;6:27220. doi: 10.1038/srep27220.
  PUBMED    27264994
REFERENCE   2  (bases 1 to 977)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-06-6"; volume: "6:27220. doi"; pages: " 
            10.1038/srep27220"
COMMENT     Sequence Label: pAav-MDM2-PQS2-3xHA
FEATURES             Location/Qualifiers
     source          1..977
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     2..25
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     protein_bind    72..105
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(133..222)
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     protein_bind    complement(296..343)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
ORIGIN
        1 ccagcggggc tgctaaagcg catgctccag actgccttgg gaaaagcgcc tcccctaccc
       61 ggtagggcgc gataacttcg tataatgtat gctatacgaa gttatccgaa gctttcaagc
      121 ctcgggctcg ccagcgtaat ctggaacgtc atagggatag cttcctgcat agtccgggac
      181 gtcataggga tagccagcgt aatctggaac atcgtatggg tagttttctg tctccaggaa
      241 tcttccagca ccgattcctg gagaagctcc caggcctcta taagcctcgg ccactgaagt
      301 tcctatactt tctagagaat aggaacttcg gaataggaac ttcccgaatt cggggaaata
      361 agttagcaca atcatttgaa ttggttgtct acatactggg cagggcttat tccttttctt
      421 tagcttcttt gcacatgtaa agcaggccat aagatgtcct gttttgccat ggacaatgca
      481 accattttta ggtcgacctt gacaaatcac acaaggttca atggcattaa ggggcaaact
      541 agattccaca ctctcttctt tgtcttgggt ttcttccctt tcaaactctt tcacatcttc
      601 ttggctgcta taaatnatgc tactagaagt tgatggctga gaatagtctt cactttcttg
      661 tgattgtgaa gcttgtgtaa ttttatcatc attttcctca acacatgact ctctggaatc
      721 attcactata gtttttttac aatcaggaac atcaaagccc tcttcagctt gtgttgagtt
      781 ttccagtttg gctttctcag agatttcccc tttatctttc cctttatctt caggaagcca
      841 attctcacga agggcccaac atctgttgca atgtgatgga agggggggat tcatttcatt
      901 gcatgaagtg catttccaat agtccttcaa tgaaataaga cacacagtca gtttctgtaa
      961 ccctttaact gctcagt
//
