LOCUS       sequence-167079-        4631 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression of myc tagged Xenopus laevis Nup53.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4631)
  AUTHORS   Vollmer B, Schooley A, Sachdev R, Eisenhardt N, Schneider AM, 
            Sieverding C, Madlung J, Gerken U, Macek B, Antonin W
  TITLE     Dimerization and direct membrane interaction of Nup53 contribute to 
            nuclear pore complex assembly.
  JOURNAL   EMBO J. 2012 Oct 17;31(20):4072-84. doi: 10.1038/emboj.2012.256. 
            Epub 2012 Sep 7.
  PUBMED    22960634
REFERENCE   2  (bases 1 to 4631)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/emboj.2012.256"; journalName: "EMBO J"; date: "2012-10-17- 
            17"; volume: "31"; issue: "20"; pages: "4072-84"
COMMENT     Sequence Label: pSI myc Nup53xl
FEATURES             Location/Qualifiers
     source          1..4631
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..413
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        56..247
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(79..99)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      264..399
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          483..615
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     598..617
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        660..678
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             687..716
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    1745..1866
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1756..1775)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1810..1829
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      2047..2502
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2134..2153)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2344..2365
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2569..2588)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2688..2710
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2748..2766)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2834..2938
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2939..3007,3008..3799)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3157..3176)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3970..4558
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4459..4478
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcgcagcacc atggcctgaa ataacctctg aaagaggaac ttggttaggt accttctgag
       61 gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
      121 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
      181 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
      241 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
      301 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
      361 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagcttg
      421 attcttctga cacaacagtc tcgaacttaa gctgcagaag ttggtcgtga ggcactgggc
      481 aggtaagtat caaggttaca agacaggttt aaggagacca atagaaactg ggcttgtcga
      541 gacagagaag actcttgcgt ttctgatagg cacctattgg tcttactgac atccactttg
      601 cctttctctc cacaggtgtc cactcccagt tcaattacag ctcttaaggc tagagtactt
      661 aatacgactc actataggct agcatggaac aaaaacttat ttctgaagaa gatctgatga
      721 tggcagcagc atttagcatg gaaccgatgg gtgcagaacc gatggcactg ggtagcccga
      781 ccagcccgaa accgagtgcc ggtgcacagt ttctgcctgg ttttctgctg ggtgatattc
      841 cgacaccggt tacaccgcag cctcgtccga gcctgggtat tatggaagtt cgtagtccgc
      901 tgcatagcgg tggtagtcct ccgcagccgg ttctgccgac ccataaagat aaaagcggtg
      961 cacctccggt tcgtagcatt tatgatgatg ttgcaagtcc gggtctgggt agcacaccgc
     1021 gtaatacccg taaaatggca agctttagcg ttctgcatac acctctgagc ggtgcaattc
     1081 cgagcagtcc ggcaagcaat gtttttagtc cggcaaccat tggtcagagc cgtaaaacca
     1141 ccctgagtcc ggcacagatg gacccgtttt atacccaggg tgatgcactg accagtgatg
     1201 atcagctgga tgatacctgg gttaccgttt ttggttttcc gcaggcaagc gcaagctata
     1261 ttctgctgca gtttgcacag tatggcaata ttattaaaca tgtgatgagc aataatggca
     1321 attggatgca tattcagtat cagagcaaac tgcaggcacg taaagcactg agcaaagatg
     1381 gtcgtatttt tggtgaaagc attatgattg gtgtgaaacc gtgcattgat aaaagcgtta
     1441 tggaagcaac cgaaaaagtt agcaccccga gcgttagcag cgtttttaca cctccggtta
     1501 aaagcattcg taccccgacc cagagcgttg gtacaccgcg tgcagcaagc atgcgtccgc
     1561 tggcagcaac ctatcgcacc ccgaccagcg cagattatca ggttgttagc gataaaccgg
     1621 caccgcgtaa agatgaaagc attgttagca aagccatgga atatatgttt ggttggtgat
     1681 agctcgagaa ttcacgcgtg gtacctctag agtcgacccg ggcggccgct tcgagcagac
     1741 atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc
     1801 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa
     1861 caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag
     1921 gttttttaaa gcaagtaaaa cctctacaaa tgtggtaaaa tcgataagga tccgggctgg
     1981 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     2041 gaatggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     2101 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     2161 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     2221 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     2281 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     2341 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     2401 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     2461 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttcctgatgc ggtattttct
     2521 ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc
     2581 tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg
     2641 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
     2701 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg
     2761 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
     2821 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     2881 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     2941 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     3001 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     3061 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     3121 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     3181 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     3241 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     3301 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     3361 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     3421 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     3481 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     3541 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     3601 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     3661 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     3721 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     3781 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     3841 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     3901 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     3961 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     4021 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     4081 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     4141 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     4201 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     4261 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     4321 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     4381 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     4441 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     4501 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     4561 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatggc
     4621 tcgacagatc t
//
