LOCUS       sequence-16708-e        1336 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1336)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1997
REFERENCE   2  (bases 1 to 1336)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: L3606
FEATURES             Location/Qualifiers
     source          1..1336
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             467..487
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(530..558)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 gatcttacta actaactatt ctcatttaaa ttttcagagc ttaaaaatgg ctgancccnt
       61 tnanaacgat ggatacgcta acaacttgga aatgaaatcn ncttgcatgc ctgcaggtcg
      121 actctagagg atccccaatt cttgagactt ccactatctt ctctggcacc ctccactacg
      181 cgtaggcgtc tctcgcttcg tgtattcccg ggaagccggg ttcccgtctc tcccgccgct
      241 gccgctgccg cacacagctt tacacctcgt agaatcccca aagaggggcg tggcttgcgg
      301 gtgccaacat cctcctgccg aggaagaagc aggcactcat cactcgcatc atcaacctcg
      361 ggattggcca aaggacccaa aggtatgttt cgaatgatac taacataaca tagaacattt
      421 tcaggaggac ccttggaggg taccgagctc agaaaaaatg actgctccaa agaagaagcg
      481 taaggtaccg gtagaaaaaa tgagtaaagg agaagaactt ttcactggag ttgtcccaat
      541 tcttgttgaa ttagatggtg atgttaatgg gcacaaattt tctgtcagtg gagagggtga
      601 aggtgatgca acatacggaa aacttaccct taaatttatt tgcactactg gaaaactacc
      661 tgttccatgg gtaagtttaa acatatatat actaactaac cctgattatt taaattttca
      721 gccaacactt gtcactactt tcacttatgg tgttcaatgc ttctcgagat acccagatca
      781 tatgaaacag catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaagaac
      841 tatatttttc aaagatgacg ggaactacaa gacacgtaag tttaaacagt tcggtactaa
      901 ctaaccatac atatttaaat tttcaggtgc tgaagtcnag ttttgaaggt gatacccttg
      961 ttaatagaat cgagtnaaag gtatggattt taagaagatg gaaacattct tggacccaaa
     1021 ttggaataca ctatnttnnc acancgtata cttcatggca nanaancaaa gantggaatc
     1081 aagntgtagt ttaacntgat ttnctaacta actatcggat tnaattttcn aactncaaat
     1141 taaacccact ngaaanggag gntncantng cnaacttttc naaaatttcc nttggggagg
     1201 nctnttnttt ncaaaaacnt tctgnccana antgnctttt aaaatcccaa aaaaaaaccn
     1261 nggcccttng antntaancc ggggggaatn cnggggggga aantaaaaaa ntttaantcc
     1321 ngnggngngt ttatna
//
