LOCUS       sequence-167080-        4628 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression of HA tagged Xenopus laevis Nup53.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4628)
  AUTHORS   Vollmer B, Schooley A, Sachdev R, Eisenhardt N, Schneider AM, 
            Sieverding C, Madlung J, Gerken U, Macek B, Antonin W
  TITLE     Dimerization and direct membrane interaction of Nup53 contribute to 
            nuclear pore complex assembly.
  JOURNAL   EMBO J. 2012 Oct 17;31(20):4072-84. doi: 10.1038/emboj.2012.256. 
            Epub 2012 Sep 7.
  PUBMED    22960634
REFERENCE   2  (bases 1 to 4628)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/emboj.2012.256"; journalName: "EMBO J"; date: "2012-10-17- 
            17"; volume: "31"; issue: "20"; pages: "4072-84"
COMMENT     Sequence Label: pSI HA Nup53xl
FEATURES             Location/Qualifiers
     source          1..4628
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..413
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        56..247
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(79..99)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      264..399
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          483..615
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     598..617
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        660..678
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             687..713
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    1742..1863
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1753..1772)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1807..1826
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      2044..2499
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2131..2150)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2341..2362
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2566..2585)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2685..2707
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2745..2763)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2831..2935
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2936..3004,3005..3796)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3154..3173)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3967..4555
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4456..4475
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcgcagcacc atggcctgaa ataacctctg aaagaggaac ttggttaggt accttctgag
       61 gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
      121 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
      181 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
      241 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
      301 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
      361 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagcttg
      421 attcttctga cacaacagtc tcgaacttaa gctgcagaag ttggtcgtga ggcactgggc
      481 aggtaagtat caaggttaca agacaggttt aaggagacca atagaaactg ggcttgtcga
      541 gacagagaag actcttgcgt ttctgatagg cacctattgg tcttactgac atccactttg
      601 cctttctctc cacaggtgtc cactcccagt tcaattacag ctcttaaggc tagagtactt
      661 aatacgactc actataggct agcatgtacc catacgacgt cccagactac gctatgatgg
      721 cagcagcatt tagcatggaa ccgatgggtg cagaaccgat ggcactgggt agcccgacca
      781 gcccgaaacc gagtgccggt gcacagtttc tgcctggttt tctgctgggt gatattccga
      841 caccggttac accgcagcct cgtccgagcc tgggtattat ggaagttcgt agtccgctgc
      901 atagcggtgg tagtcctccg cagccggttc tgccgaccca taaagataaa agcggtgcac
      961 ctccggttcg tagcatttat gatgatgttg caagtccggg tctgggtagc acaccgcgta
     1021 atacccgtaa aatggcaagc tttagcgttc tgcatacacc tctgagcggt gcaattccga
     1081 gcagtccggc aagcaatgtt tttagtccgg caaccattgg tcagagccgt aaaaccaccc
     1141 tgagtccggc acagatggac ccgttttata cccagggtga tgcactgacc agtgatgatc
     1201 agctggatga tacctgggtt accgtttttg gttttccgca ggcaagcgca agctatattc
     1261 tgctgcagtt tgcacagtat ggcaatatta ttaaacatgt gatgagcaat aatggcaatt
     1321 ggatgcatat tcagtatcag agcaaactgc aggcacgtaa agcactgagc aaagatggtc
     1381 gtatttttgg tgaaagcatt atgattggtg tgaaaccgtg cattgataaa agcgttatgg
     1441 aagcaaccga aaaagttagc accccgagcg ttagcagcgt ttttacacct ccggttaaaa
     1501 gcattcgtac cccgacccag agcgttggta caccgcgtgc agcaagcatg cgtccgctgg
     1561 cagcaaccta tcgcaccccg accagcgcag attatcaggt tgttagcgat aaaccggcac
     1621 cgcgtaaaga tgaaagcatt gttagcaaag ccatggaata tatgtttggt tggtgatagc
     1681 tcgagaattc acgcgtggta cctctagagt cgacccgggc ggccgcttcg agcagacatg
     1741 ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt
     1801 atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa
     1861 gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt
     1921 ttttaaagca agtaaaacct ctacaaatgt ggtaaaatcg ataaggatcc gggctggcgt
     1981 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     2041 tggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     2101 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     2161 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     2221 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     2281 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     2341 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     2401 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     2461 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc ctgatgcggt attttctcct
     2521 tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga
     2581 tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
     2641 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
     2701 tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct
     2761 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
     2821 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     2881 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     2941 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     3001 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     3061 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     3121 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     3181 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     3241 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     3301 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     3361 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     3421 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     3481 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     3541 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     3601 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     3661 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     3721 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     3781 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     3841 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     3901 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     3961 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     4021 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     4081 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     4141 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     4201 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     4261 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     4321 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     4381 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     4441 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     4501 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     4561 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatggctcg
     4621 acagatct
//
