LOCUS       sequence-167083-         837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MESA target chain with V1-MESA ectodomain, 45 extracellular linkers,
            a flag tag, M cleavage sequence, and tTA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Schwarz KA, Daringer NM, Dolberg TB, Leonard JN
  TITLE     Rewiring human cellular input-output using modular extracellular 
            sensors.
  JOURNAL   Nat Chem Biol. 2016 Dec 12. doi: 10.1038/nchembio.2253.
  PUBMED    27941759
REFERENCE   2  (bases 1 to 837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2016 Dec 12. doi: 10.1038/nchembio.2253."
COMMENT     Sequence Label: V1-MESA-45F-M-tTA
FEATURES             Location/Qualifiers
     source          1..837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agcgccatca tggcttggac cagcttaatt cttagcttgc ttgccctgtg
      121 cagcggtgca tcatctgaca tacagatgac tcagtcacct agcagtctgt ctgcttcagt
      181 cggtgatagg gtaactatca cctgtcgggc atctcaggat gtgtccacgg cagttgcctg
      241 gtaccagcag aagcccggca aggcgcctaa gttgcttatc tattcagctt catttttgta
      301 tagtggggtg cctagccggt ttagcggaag tggttctggc acagacttca ctttgacaat
      361 cagttccctc caaccggagg atttcgctac ctactactgt cagcaggggt acggaaaccc
      421 ctttacgttt ggacagggca ctaaagtgga gattaagagg ggaggcggag gctccggtgg
      481 gggaggctct ggtggtggag gtagcgaggt tcagctggtg gaaagtggtg gcggactagt
      541 acagccaggc ggctccctgc ggctgagttg cgccgcgagt ggctttacta tatctgacta
      601 ttggatccat tgggtcaggc aagcccctgg gaaaggattg gagtgggttg ctggtattac
      661 ccccgcaggt gggtatactt attatgcaga tagcgtgaaa gggaggttca ccatctctgc
      721 agacacaagc aagaacacgg cctacctaca gatgaactct ctgagggcag aggacactgc
      781 cgtctactat tgcgcccgat ttgtgttttt tctaccctac gccatggact attgggg
//
