LOCUS       sequence-167090-         788 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MESA target chain with V2-MESA ectodomain, 35 extracellular linkers,
            a flag tag, M cleavage sequence, and dCas9-VP64.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Schwarz KA, Daringer NM, Dolberg TB, Leonard JN
  TITLE     Rewiring human cellular input-output using modular extracellular 
            sensors.
  JOURNAL   Nat Chem Biol. 2016 Dec 12. doi: 10.1038/nchembio.2253.
  PUBMED    27941759
REFERENCE   2  (bases 1 to 788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2016 Dec 12. doi: 10.1038/nchembio.2253."
COMMENT     Sequence Label: V2-MESA-35F-M-dCas9
FEATURES             Location/Qualifiers
     source          1..788
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     LTR             14..194
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    241..366
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
ORIGIN
        1 ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
       61 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
      121 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
      181 gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag
      241 ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga
      301 ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag
      361 agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca
      421 gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga
      481 ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag
      541 ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca
      601 accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag
      661 atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag
      721 acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
      781 aaaaattg
//
