LOCUS       sequence-167092-        5583 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing RBM10 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5583)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 5583)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_RBM10_p.R171W
FEATURES             Location/Qualifiers
     source          1..5583
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(929..946)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(1545..1556)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(2983..3082)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3099..3118)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3100..3118)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3123..3139)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3123..3139)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3570..4361
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4454..5042
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4943..4962
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5196..5213
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(5372..5399)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(5491..5577)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcac catggagtat gaaagacgtg gtggtcgtgg tgacaggact ggccgctatg
      241 gagccactga ccgctcgcag gatgatggtg gggagaaccg cagccgagac cacgactacc
      301 gggacatgga ctaccgttca tatcctcgcg agtatggcag ccaggagggc aagcatgact
      361 atgacgactc atctgaggag cagagtgcgg aggattccta cgaggcctcc ccgggctccg
      421 agactcagcg taggcggcgg cggcggcaca ggcacagccc caccggcccg ccaggcttcc
      481 cccgagacgg cgactatcgg gaccaggact atcggaccga gcaaggggag gaggaggagg
      541 aggaggagga tgaggaggag gaggagaagg ccagtaacat cgtcatgctg aggatgctgc
      601 cacaggcagc cactgaggat gacatccgtg gccagctgca gtcgcacggc gtgcaagcac
      661 gggaggttcg gctgatgcgg aacaaatctt caggtcagag ctggggcttc gccttcgtcg
      721 agtttagtca cttgcaggac gctacacgat ggatggaagc caatcagcac tccctcaaca
      781 tcctgggcca gaaggtgtcg atgcactaca gtgaccccaa gcccaagatc aatgaggact
      841 ggctgtgcaa taagtgtggc gtccagaact tcaaacgccg agagaagtgc ttcaaatgtg
      901 gcgtgcccaa gtcagaggca gagcagaagc tgcccctcgg cacgaggctg gatcagcaga
      961 cactgccact gggtggccgg gagctgagcc agggcctgct tcccctgccg cagccctacc
     1021 aggcccaggg agtcctggcc tcccaagccc tgtcacaggg ctcggagcca agctcagaga
     1081 acgccaatga caccatcatt ttgcgcaacc tgaacccaca cagcaccatg gattccatcc
     1141 tgggggccct ggcaccctac gcggtgctgt cctcctccaa cgtgcgcgtc ataaaggaca
     1201 agcagaccca actgaaccgc ggctttgcct tcatccagct ctccaccatc gtggaggcag
     1261 cccagctgct gcagatcctg caggccctgc acccaccact cactatcgac ggcaagacca
     1321 tcaatgttga gtttgccaag ggttctaaga gggacatggc ctccaatgaa ggcagtcgca
     1381 tcagtgctgc ctctgtggcc agcactgcca ttgctgcggc ccagtgggcc atctcacagg
     1441 cctcccaagg tggggagggt acctgggcca cctccgagga gccgccggtc gactacagct
     1501 actaccaaca ggatgagggc tatggcaaca gccagggcac agagtcttcc ctctatgccc
     1561 atggctacct caagggcacc aagggccctg gcatcactgg aaccaaaggg gatcccactg
     1621 gagcaggtcc cgaggcctcc ctagagcctg gggccgactc tgtgtcgatg caggctttct
     1681 ctcgcgccca gcctggtgct gctcctggca tctaccaaca atcagccgag gcgagcagta
     1741 gccagggcac tgctgccaac agccagtcgt ataccatcat gtcacccgct gtgctcaaat
     1801 ctgagctcca gagccctacc catcctagtt ctgctctccc accggctacc agccccactg
     1861 cccaggaatc ctacagccag taccctgttc ccgacgtctc tacctaccag tacgatgaga
     1921 cctccggcta ctactatgac ccccagaccg gcctctacta tgaccccaac tcccagtatt
     1981 actacaatgc tcagagccag cagtacctgt actgggatgg ggagaggcgg acctatgttc
     2041 ccgccctgga gcagtcggcc gacggacata aggagacagg ggcaccctcg aaggagggca
     2101 aagagaagaa ggagaagcac aagaccaaga cagctcaaca gattgccaag gacatggaac
     2161 gctgggcccg cagtctcaac aaacaaaaag aaaacttcaa aaatagcttc cagcctatca
     2221 gctccctgcg agatgacgag aggcgggagt cagccactgc agatgctggc tatgccatcc
     2281 tcgagaagaa gggagcacta gccgagagac agcacaccag catggatctc ccgaaattgg
     2341 ccagtgacga ccgcccaagc cctccgcgag gactggtggc agcctacagc ggggagagtg
     2401 acagtgagga ggagcaggag cgtgggggcc ctgagcggga ggagaagctc accgactggc
     2461 agaagctggc ctgtctgctc tgccgacgcc agttccccag caaagaggcg ctcatccggc
     2521 accagcagct ctcagggctc cacaagcaaa accttgagat tcaccggcga gcccacttgt
     2581 cagaaaacga gctagaagca ctagagaaga atgacatgga gcaaatgaag taccgggacc
     2641 gtgcagctga acgcagagaa aagtatggca tccccgagcc gccagagccc aagaggagga
     2701 agtacggcgg catatccaca gcctctgtag acttcgagca gcctactcgg gacgggctgg
     2761 gcagtgacaa cattggcagt cggatgctgc aggccatggg ctggaaagag ggcagcggcc
     2821 tgggccgcaa gaagcagggc attgtaacgc ctatcgaggc ccaaacacgg gtgcggggct
     2881 ccggcctggg tgcacggggc agctcctacg gggtcacctc aaccgagtcc tacaaggaga
     2941 cactgcacaa gacaatggtg acccgcttca acgaggccca gtgcccaact ttcttgtaca
     3001 aagttggcat tataagaaag cattgcttat caatttgttg caacgaacag gtcactatca
     3061 gtcaaaataa aatcattatt tgccatccag ctgatatccc ctatagtgag tcgtattaca
     3121 tggtcatagc tgtttcctgg cagctctggc ccgtgtctca aaatctctga tgttacattg
     3181 cacaagataa aaatatatca tcatgcctcc tctagaccag ccaggacaga aatgcctcga
     3241 cttcgctgct acccaaggtt gccgggtgac gcacaccgtg gaaacggatg aaggcacgaa
     3301 cccagtggac ataagcctgt tcggttcgta agctgtaatg caagtagcgt atgcgctcac
     3361 gcaactggtc cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca
     3421 tggcttgtta tgactgtttt tttggggtac agtctatgcc tcgggcatcc aagcagcaag
     3481 cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg
     3541 gcagtcgccc taaaacaaag ttaaacatta tgagggaagc ggtgatcgcc gaagtatcga
     3601 ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg
     3661 tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc
     3721 tggttacggt gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt
     3781 tggaaacttc ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg
     3841 ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag
     3901 aatggcagcg caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc
     3961 tggctatctt gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg
     4021 aggaactctt tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa
     4081 cgctatggaa ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt
     4141 cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact
     4201 gggcaatgga gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt
     4261 atcttggaca agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc
     4321 actacgtgaa aggcgagatc accaaggtag tcggcaaata accctcgagc cacccatgac
     4381 caaaatccct taacgtgagt tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga
     4441 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     4501 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     4561 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     4621 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     4681 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     4741 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     4801 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     4861 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     4921 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     4981 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     5041 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     5101 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     5161 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     5221 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     5281 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatacgcgta
     5341 ccgctagcca ggaagagttt gtagaaacgc aaaaaggcca tccgtcagga tggccttctg
     5401 cttagtttga tgcctggcag tttatggcgg gcgtcctgcc cgccaccctc cgggccgttg
     5461 cttcacaacg ttcaaatccg ctcccggcgg atttgtccta ctcaggagag cgttcaccga
     5521 caaacaacag ataaaacgaa aggcccagtc ttccgactga gcctttcgtt ttatttgatg
     5581 cct
//
