LOCUS       sequence-167094-        5442 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing CHSY3 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5442)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 5442)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_CHSY3_p.R350H
FEATURES             Location/Qualifiers
     source          1..5442
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2842..2941)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2958..2977)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2959..2977)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2982..2998)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2982..2998)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3429..4220
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4313..4901
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4802..4821
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5055..5072
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(5231..5258)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(5350..5436)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcac catggctgtg cgctctcgcc gcccgtggat gagcgtggca ttagggctgg
      241 tgctgggctt caccgccgcg tcctggctca tcgcccccag ggtggcggag ctgagcgaga
      301 ggaagagacg tggctccagc ctctgctcct actacggtcg ctctgctgct ggcccccgcg
      361 ccggcgctca gcagccgctc ccccagcccc agtcccgacc acggcaggag cagtcgccgc
      421 cccccgcgcg ccaggatctc caggggccac cgctgcccga ggcagcaccc gggatcacca
      481 gttttcgaag cagcccctgg cagcagccac ctccgctgca gcagcggcgg cgaggacgcg
      541 agcctgaggg cgcgacgggg cttcccggtg ctccagcggc cgagggggag cccgaggagg
      601 aggacggggg cgcggctgga cagcggagag acggccggcc ggggagtagc cacaacggca
      661 gcggggacgg gggcgctgcc gccccgagcg cccgaccccg ggacttcctg tacgtggggg
      721 tgatgaccgc gcagaagtac ctgggcagcc gcgcgctggc cgcgcagcgg acctgggcgc
      781 gtttcatccc gggccgcgtg gagttctttt ccagccagca gccccccaac gccggccagc
      841 ccccgccacc cctgcctgtc atcgcgctac cgggtgtgga cgactcctat cctccccaga
      901 aaaagtcctt catgatgatc aagtacatgc acgaccacta cctggacaag tatgagtggt
      961 tcatgcgcgc cgacgacgat gtctacatca aaggtgataa attagaagag tttcttagat
     1021 cgctaaacag cagtaagcct ctctacctgg gacagactgg cctggggaat attgaagagc
     1081 ttggaaagct gggactggag cctggggaaa acttctgtat gggaggacct ggcatgatct
     1141 ttagccgaga agttctcagg aggatggtgc cacatattgg tgaatgcctt agagaaatgt
     1201 acacgactca tgaggatgtg gaagtaggaa gatgcgttca ccgttttggt gggactcagt
     1261 gtgtctggtc ttacgagatg caacaactgt tccatgaaaa ttatgaacac aatcggaagg
     1321 gttacatcca agaccttcac aatagcaaaa tccatgcagc cataacactt catcccaaca
     1381 aaaggcctgc ataccaatac aggctgcata attacatgct cagccgcaaa atttctgaac
     1441 ttcgctaccg caccatccag ctccacaggg aaagtgccct gatgagcaag ctcagtaaca
     1501 cagaagtgag caaagaggac cagcagctgg gagtgatacc ttctttcaac cacttccagc
     1561 ctcgggagag aaatgaagtg atagaatggg agttcctgac agggaagctt ctatactcag
     1621 cagctgagaa ccagccccct cgacagagcc tcagtagcat tttaagaaca gcactggatg
     1681 ataccgtcct acaggtgatg gagatgatca atgagaatgc caagagcaga ggacggctca
     1741 ttgacttcaa ggaaattcag tatggctacc gcagagttaa ccccatgcac ggggtggagt
     1801 acattttgga tttactcctt ttatacaaaa gacacaaggg aaggaaactg actgtgccag
     1861 tgagacgtca tgcctatctt cagcagttgt tcagcaagcc tttcttcaga gagaccgaag
     1921 agctagatgt caacagtctt gtggagagta ttaacagtga aactcagtca ttctccttta
     1981 tatctaattc tttaaagata ttatcttctt ttcaaggtgc caaagaaatg ggagggcaca
     2041 atgaaaagaa agtacacatt ctcgttcctc tcatcggaag gtatgacatt ttcttgagat
     2101 tcatggagaa ctttgaaaac atgtgtctta tcccaaagca gaatgtaaag ttggtcatta
     2161 tccttttcag tagggattct ggccaagact ccagcaagca tattgagctg ataaaagggt
     2221 accagaacaa gtaccccaaa gcagaaatga ccctgatccc aatgaaggga gagttttcca
     2281 gaggtcttgg tcttgaaatg gcttctgccc agtttgacaa tgacactttg ctgctatttt
     2341 gtgatgttga cttgatcttc agagaagatt ttctccaacg atgtagagac aatacaattc
     2401 agggacaaca ggtgtactat cccatcatct ttagccagta tgacccaaag gtaacaaacg
     2461 ggggaaatcc tcccactgat gattacttca tattctcaaa aaagactgga ttttggagag
     2521 actatggata tggcatcacc tgtatttaca aaagtgatct tctaggtgca ggtggatttg
     2581 atacctcaat acaaggctgg ggactagaag atgtagatct ctacaataaa gtcattctat
     2641 ctggcttaag gccattcaga agccaagaag taggagtggt gcatattttc catccagttc
     2701 attgtgatcc taacttggac cctaagcagt ataagatgtg cttaggatcc aaggcaagta
     2761 ctttcgcctc aaccatgcaa ctggctgaac tctggcttga aaaacattta ggtgtcaggt
     2821 acaatcgaac tctctcctga ttgccaactt tcttgtacaa agttggcatt ataagaaagc
     2881 attgcttatc aatttgttgc aacgaacagg tcactatcag tcaaaataaa atcattattt
     2941 gccatccagc tgatatcccc tatagtgagt cgtattacat ggtcatagct gtttcctggc
     3001 agctctggcc cgtgtctcaa aatctctgat gttacattgc acaagataaa aatatatcat
     3061 catgcctcct ctagaccagc caggacagaa atgcctcgac ttcgctgcta cccaaggttg
     3121 ccgggtgacg cacaccgtgg aaacggatga aggcacgaac ccagtggaca taagcctgtt
     3181 cggttcgtaa gctgtaatgc aagtagcgta tgcgctcacg caactggtcc agaaccttga
     3241 ccgaacgcag cggtggtaac ggcgcagtgg cggttttcat ggcttgttat gactgttttt
     3301 ttggggtaca gtctatgcct cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat
     3361 gtttgatgtt atggagcagc aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt
     3421 taaacattat gagggaagcg gtgatcgccg aagtatcgac tcaactatca gaggtagttg
     3481 gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt acatttgtac ggctccgcag
     3541 tggatggcgg cctgaagcca cacagtgata ttgatttgct ggttacggtg accgtaaggc
     3601 ttgatgaaac aacgcggcga gctttgatca acgacctttt ggaaacttcg gcttcccctg
     3661 gagagagcga gattctccgc gctgtagaag tcaccattgt tgtgcacgac gacatcattc
     3721 cgtggcgtta tccagctaag cgcgaactgc aatttggaga atggcagcgc aatgacattc
     3781 ttgcaggtat cttcgagcca gccacgatcg acattgatct ggctatcttg ctgacaaaag
     3841 caagagaaca tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt gatccggttc
     3901 ctgaacagga tctatttgag gcgctaaatg aaaccttaac gctatggaac tcgccgcccg
     3961 actgggctgg cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag
     4021 taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg
     4081 cccagtatca gcccgtcata cttgaagcta gacaggctta tcttggacaa gaagaagatc
     4141 gcttggcctc gcgcgcagat cagttggaag aatttgtcca ctacgtgaaa ggcgagatca
     4201 ccaaggtagt cggcaaataa ccctcgagcc acccatgacc aaaatccctt aacgtgagtt
     4261 acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     4321 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     4381 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     4441 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     4501 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     4561 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     4621 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     4681 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     4741 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     4801 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     4861 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     4921 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     4981 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     5041 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     5101 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
     5161 ggaaagcggg cagtgagcgc aacgcaatta atacgcgtac cgctagccag gaagagtttg
     5221 tagaaacgca aaaaggccat ccgtcaggat ggccttctgc ttagtttgat gcctggcagt
     5281 ttatggcggg cgtcctgccc gccaccctcc gggccgttgc ttcacaacgt tcaaatccgc
     5341 tcccggcgga tttgtcctac tcaggagagc gttcaccgac aaacaacaga taaaacgaaa
     5401 ggcccagtct tccgactgag cctttcgttt tatttgatgc ct
//
