LOCUS       sequence-167096-         624 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MESA target chain with V2-MESA ectodomain, 35 extracellular linkers,
            a flag tag, M cleavage sequence, and dCas9-VP64.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 624)
  AUTHORS   Schwarz KA, Daringer NM, Dolberg TB, Leonard JN
  TITLE     Rewiring human cellular input-output using modular extracellular 
            sensors.
  JOURNAL   Nat Chem Biol. 2016 Dec 12. doi: 10.1038/nchembio.2253.
  PUBMED    27941759
REFERENCE   2  (bases 1 to 624)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2016 Dec 12. doi: 10.1038/nchembio.2253."
COMMENT     Sequence Label: V2-MESA-35F-M-dCas9
FEATURES             Location/Qualifiers
     source          1..624
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(4..57)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(79..228)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             complement(253..273)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 cattgggcca ggattctcct cgacgtcacc gcatgttagc agacttcctc tgccctctcc
       61 actgcctgta cagttaatca gcatgtccag gtcgaaatca tcaagggcgt cactgccgag
      121 catgtcgagg tcaaagtcat caagggcatc cgaaccaagc atgtcaaggt caaaatcatc
      181 gagggcgtca cttcccagca tatccagatc aaaatcgtcc aatgcgtcag cccgtccgga
      241 tccagcggcc gccaccttcc tctttttctt aggtccgcta cctcctcctc cgctncctcc
      301 tcctccgctt ccacctcctc cagcgctgtc gcctcccagc tgagacaggt cgatccgtgt
      361 ctcgtacagg ccggtgatgc tctggtggat cagggtggcg tccagcacct ctttggtgct
      421 ggtgtacctc ttccggtcga tggtggtgtc aaagtacttg aaggcggcag gggctcccag
      481 attggtcagg gtaaacaggt ggatgatatt ctcggcctgc tctctgatgg gcttatcccg
      541 gtgcttgttg taggcggaca gcactttgtc cagattagcg tcggccagga tcactctctt
      601 ggagaactcg ctgatctgct cgat
//
