LOCUS       sequence-167098-        5529 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing DNMT3A , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5529)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 5529)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_DNMT3A_p.Q485H
FEATURES             Location/Qualifiers
     source          1..5529
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             192..2927
                     /codon_start=1
                     /product="DNA methyltransferase 3A"
                     /label=DNMT3A
                     /translation="MPAMPSSGPGDTSSSAAEREEDRKDGEEQEEPRGKEERQEPSTTA
                     RKVGRPGRKRKHPPVESGDTPKDPAVISKSPSMAQDSGASELLPNGDLEKRSEPQPEEG
                     SPAGGQKGGAPAEGEGAAETLPEASRAVENGCCTPKEGRGAPAEAGKEQKETNIESMKM
                     EGSRGRLRGGLGWESSLRQRPMPRLTFQAGDPYYISKRKRDEWLARWKREAEKKAKVIA
                     GMNAVEENQGPGESQKVEEASPPAVQQPTDPASPTVATTPEPVGSDAGDKNATKAGDDE
                     PEYEDGRGFGIGELVWGKLRGFSWWPGRIVSWWMTGRSRAAEGTRWVMWFGDGKFSVVC
                     VEKLMPLSSFCSAFHQATYNKQPMYRKAIYEVLQVASSRAGKLFPVCHDSDESDTAKAV
                     EVQNKPMIEWALGGFQPSGPKGLEPPEEEKNPYKEVYTDMWVEPEAAAYAPPPPAKKPR
                     KSTAEKPKVKEIIDERTRERLVYEVRHKCRNIEDICISCGSLNVTLEHPLFVGGMCQNC
                     KNCFLECAYQYDDDGYQSYCTICCGGREVLMCGNNNCCRCFCVECVDLLVGPGAAQAAI
                     KEDPWNCYMCGHKGTYGLLRRREDWPSRLQMFFANNHDQEFDPPKVYPPVPAEKRKPIR
                     VLSLFDGIATGLLVLKDLGIQVDRYIASEVCEDSITVGMVRHQGKIMYVGDVRSVTQKH
                     IQEWGPFDLVIGGSPCNDLSIVNPARKGLYEGTGRLFFEFYRLLHDARPKEGDDRPFFW
                     LFENVVAMGVSDKRDISRFLESNPVMIDAKEVSAAHRARYFWGNLPGMNRPLASTVNDK
                     LELQECLEHGRIAKFSKVRTITTRSNSIKQGKDQHFPVFMNEKEDILWCTEMERVFGFP
                     VHYTDVSNMSRLARQRLLGRSWSVPVIRHLFAPLKEYFACV"
     protein_bind    complement(2929..3028)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3045..3064)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3046..3064)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3069..3085)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3069..3085)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3516..4307
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4400..4988
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4889..4908
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5142..5159
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(5318..5345)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(5437..5523)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcac catgcccgcc atgccctcca gcggccccgg ggacaccagc agctctgctg
      241 cggagcggga ggaggaccga aaggacggag aggagcagga ggagccgcgt ggcaaggagg
      301 agcgccaaga gcccagcacc acggcacgga aggtggggcg gcctgggagg aagcgcaagc
      361 accccccggt ggaaagcggt gacacgccaa aggaccctgc ggtgatctcc aagtccccat
      421 ccatggccca ggactcaggc gcctcagagc tattacccaa tggggacttg gagaagcgga
      481 gtgagcccca gccagaggag gggagccctg ctggggggca gaagggcggg gccccagcag
      541 agggagaggg tgcagctgag accctgcctg aagcctcaag agcagtggaa aatggctgct
      601 gcacccccaa ggagggccga ggagcccctg cagaagcggg caaagaacag aaggagacca
      661 acatcgaatc catgaaaatg gagggctccc ggggccggct gcggggtggc ttgggctggg
      721 agtccagcct ccgtcagcgg cccatgccga ggctcacctt ccaggcgggg gacccctact
      781 acatcagcaa gcgcaagcgg gacgagtggc tggcacgctg gaaaagggag gctgagaaga
      841 aagccaaggt cattgcagga atgaatgctg tggaagaaaa ccaggggccc ggggagtctc
      901 agaaggtgga ggaggccagc cctcctgctg tgcagcagcc cactgacccc gcatccccca
      961 ctgtggctac cacgcctgag cccgtggggt ccgatgctgg ggacaagaat gccaccaaag
     1021 caggcgatga cgagccagag tacgaggacg gccggggctt tggcattggg gagctggtgt
     1081 gggggaaact gcggggcttc tcctggtggc caggccgcat tgtgtcttgg tggatgacgg
     1141 gccggagccg agcagctgaa ggcacccgct gggtcatgtg gttcggagac ggcaaattct
     1201 cagtggtgtg tgttgagaag ctgatgccgc tgagctcgtt ttgcagtgcg ttccaccagg
     1261 ccacgtacaa caagcagccc atgtaccgca aagccatcta cgaggtcctg caggtggcca
     1321 gcagccgcgc ggggaagctg ttcccggtgt gccacgacag cgatgagagt gacactgcca
     1381 aggccgtgga ggtgcagaac aagcccatga ttgaatgggc cctggggggc ttccagcctt
     1441 ctggccctaa gggcctggag ccaccagaag aagagaagaa tccctacaaa gaagtgtaca
     1501 cggacatgtg ggtggaacct gaggcagctg cctacgcacc acctccacca gccaaaaagc
     1561 cccggaagag cacagcggag aagcccaagg tcaaggagat tattgatgag cgcacaagag
     1621 agcggctggt gtacgaggtg cgacacaagt gccggaacat tgaggacatc tgcatctcct
     1681 gtgggagcct caatgttacc ctggaacacc ccctcttcgt tggaggaatg tgccaaaact
     1741 gcaagaactg ctttctggag tgtgcgtacc agtacgacga cgacggctac cagtcctact
     1801 gcaccatctg ctgtgggggc cgtgaggtgc tcatgtgcgg aaacaacaac tgctgcaggt
     1861 gcttttgcgt ggagtgtgtg gacctcttgg tggggccggg ggctgcccag gcagccatta
     1921 aggaagaccc ctggaactgc tacatgtgcg ggcacaaggg tacctacggg ctgctgcggc
     1981 ggcgagagga ctggccctcc cggctccaga tgttcttcgc taataaccac gaccaggaat
     2041 ttgaccctcc aaaggtttac ccacctgtcc cagctgagaa gaggaagccc atccgggtgc
     2101 tgtctctctt tgatggaatc gctacagggc tcctggtgct gaaggacttg ggcattcagg
     2161 tggaccgcta cattgcctcg gaggtgtgtg aggactccat cacggtgggc atggtgcggc
     2221 accaggggaa gatcatgtac gtcggggacg tccgcagcgt cacacagaag catatccagg
     2281 agtggggccc attcgatctg gtgattgggg gcagtccctg caatgacctc tccatcgtca
     2341 accctgctcg caagggcctc tacgagggca ctggccggct cttctttgag ttctaccgcc
     2401 tcctgcatga tgcgcggccc aaggagggag atgatcgccc cttcttctgg ctctttgaga
     2461 atgtggtggc catgggcgtt agtgacaaga gggacatctc gcgatttctc gagtccaacc
     2521 ctgtgatgat tgatgccaaa gaagtgtcag ctgcacacag ggcccgctac ttctggggta
     2581 accttcccgg tatgaacagg ccgttggcat ccactgtgaa tgataagctg gagctgcagg
     2641 agtgtctgga gcatggcagg atagccaagt tcagcaaagt gaggaccatt actacgaggt
     2701 caaactccat aaagcagggc aaagaccagc attttcctgt cttcatgaat gagaaagagg
     2761 acatcttatg gtgcactgaa atggaaaggg tatttggttt cccagtccac tatactgacg
     2821 tctccaacat gagccgcttg gcgaggcaga gactgctggg ccggtcatgg agcgtgccag
     2881 tcatccgcca cctcttcgct ccgctgaagg agtattttgc gtgtgtgtac ccaactttct
     2941 tgtacaaagt tggcattata agaaagcatt gcttatcaat ttgttgcaac gaacaggtca
     3001 ctatcagtca aaataaaatc attatttgcc atccagctga tatcccctat agtgagtcgt
     3061 attacatggt catagctgtt tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt
     3121 acattgcaca agataaaaat atatcatcat gcctcctcta gaccagccag gacagaaatg
     3181 cctcgacttc gctgctaccc aaggttgccg ggtgacgcac accgtggaaa cggatgaagg
     3241 cacgaaccca gtggacataa gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc
     3301 gctcacgcaa ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg
     3361 ttttcatggc ttgttatgac tgtttttttg gggtacagtc tatgcctcgg gcatccaagc
     3421 agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc
     3481 agcagggcag tcgccctaaa acaaagttaa acattatgag ggaagcggtg atcgccgaag
     3541 tatcgactca actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc
     3601 tggccgtaca tttgtacggc tccgcagtgg atggcggcct gaagccacac agtgatattg
     3661 atttgctggt tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg
     3721 accttttgga aacttcggct tcccctggag agagcgagat tctccgcgct gtagaagtca
     3781 ccattgttgt gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat
     3841 ttggagaatg gcagcgcaat gacattcttg caggtatctt cgagccagcc acgatcgaca
     3901 ttgatctggc tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag
     3961 cggcggagga actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa
     4021 ccttaacgct atggaactcg ccgcccgact gggctggcga tgagcgaaat gtagtgctta
     4081 cgttgtcccg catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg
     4141 ccgactgggc aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctagac
     4201 aggcttatct tggacaagaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat
     4261 ttgtccacta cgtgaaaggc gagatcacca aggtagtcgg caaataaccc tcgagccacc
     4321 catgaccaaa atcccttaac gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag
     4381 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     4441 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     4501 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     4561 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     4621 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     4681 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     4741 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     4801 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     4861 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     4921 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     4981 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     5041 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     5101 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     5161 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     5221 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata
     5281 cgcgtaccgc tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc
     5341 cttctgctta gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg
     5401 ccgttgcttc acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt
     5461 caccgacaaa caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat
     5521 ttgatgcct
//
