LOCUS       sequence-167101-        6006 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing PIK3CB , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6006)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 6006)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_PIK3CB_p.E497D
FEATURES             Location/Qualifiers
     source          1..6006
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     RBS             400..408
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             complement(1707..1718)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(3406..3505)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3522..3541)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3523..3541)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3546..3562)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3546..3562)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3993..4784
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4877..5465
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5366..5385
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5619..5636
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(5795..5822)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(5914..6000)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcac catgtgcttc agtttcataa tgcctcctgc tatggcagac atccttgaca
      241 tctgggcggt ggattcacag atagcatctg atggctccat acctgtggat ttccttttgc
      301 ccactgggat ttatatccag ttggaggtac ctcgggaagc taccatttct tatattaagc
      361 agatgttatg gaagcaagtt cacaattacc caatgttcaa cctccttatg gatattgact
      421 cctatatgtt tgcatgtgtg aatcagactg ctgtatatga ggagcttgaa gatgaaacac
      481 gaagactctg tgatgtcaga ccttttcttc cagttctcaa attagtgaca agaagttgtg
      541 acccagggga aaaattagac tcaaaaattg gagtccttat aggaaaaggt ctgcatgaat
      601 ttgattcctt gaaggatcct gaagtaaatg aatttcgaag aaaaatgcgc aaattcagcg
      661 aggaaaaaat cctgtcactt gtgggattgt cttggatgga ctggctaaaa caaacatatc
      721 caccagagca tgaaccatcc atccctgaaa acttagaaga taaactttat gggggaaagc
      781 tcatcgtagc tgttcatttt gaaaactgcc aggacgtgtt tagctttcaa gtgtctccta
      841 atatgaatcc tatcaaagta aatgaattgg caatccaaaa acgtttgact attcatggga
      901 aggaagatga agttagcccc tatgattatg tgttgcaagt cagcgggaga gtagaatatg
      961 tttttggtga tcatccacta attcagttcc agtatatccg gaactgtgtg atgaacagag
     1021 ccctgcccca ttttatactt gtggaatgct gcaagatcaa gaaaatgtat gaacaagaaa
     1081 tgattgccat agaggctgcc ataaatcgaa attcatctaa tcttcctctt ccattaccac
     1141 caaagaaaac acgaattatt tctcatgttt gggaaaataa caaccctttc caaattgtct
     1201 tggttaaggg aaataaactt aacacagagg aaactgtaaa agttcatgtc agggctggtc
     1261 tttttcatgg tactgagctc ctgtgtaaaa ccatcgtaag ctcagaggta tcagggaaaa
     1321 atgatcatat ttggaatgaa ccactggaat ttgatattaa tatttgtgac ttaccaagaa
     1381 tggctcgatt atgttttgct gtttatgcag ttttggataa agtaaaaacg aagaaatcaa
     1441 cgaaaactat taatccctct aaatatcaga ccatcaggaa agctggaaaa gtgcattatc
     1501 ctgtagcgtg ggtaaatacg atggtttttg actttaaagg acaattgaga actggagaca
     1561 taatattaca cagctggtct tcatttcctg atgaactcga agaaatgttg aatccaatgg
     1621 gaactgttca aacaaatcca tatactgaaa atgcaacagc tttgcatgtt aaatttccag
     1681 acaataaaaa acaaccttat tattaccctc ccttcgataa gattattgaa aaggcagctg
     1741 agattgcaag cagtgatagt gctaatgtgt caagtcgagg tggaaaaaag tttcttcctg
     1801 tattgaaaga aatcttggac agggatccct tgtctcaact gtgtgaaaat gaaatggatc
     1861 ttatttggac tttgcgacaa gactgccgag agattttccc acaatcactg ccaaaattac
     1921 tgctgtcaat caagtggaat aaacttgagg atgttgctca gcttcaggcg ctgcttcaga
     1981 tttggcctaa actgcccccc cgggaggccc tagagcttct ggatttcaac tatccagacc
     2041 agtacgttcg agaatatgct gtaggctgcc tgcgacagat gagtgatgaa gaactttctc
     2101 aatatctttt acaactggtg caagtgttaa aatatgagcc ttttcttgat tgtgccctct
     2161 ctagattcct attagaaaga gcacttggta atcggaggat agggcagttt ctattttggc
     2221 atcttaggtc agaagtgcac attcctgctg tctcagtaca atttggtgtc atccttgaag
     2281 catactgccg gggaagtgtg gggcacatga aagtgctttc taagcaggtt gaagcactca
     2341 ataagttaaa aactttaaat agtttaatca aactgaatgc cgtgaagtta aacagagcca
     2401 aagggaagga ggccatgcat acctgtttaa aacagagtgc ttaccgggaa gccctctctg
     2461 acctgcggtc acccctgaac ccatgtgtta tcctctcaga actctatgtt gaaaagtgca
     2521 aatacatgga ttccaaaatg aagcctttgt ggctggtata caataacaag gtatttggtg
     2581 aggattcagt tggagtgatt tttaaaaatg gtgatgattt acgacaggat atgttgacac
     2641 tccaaatgtt gcgcttgatg gatttactct ggaaagaagc tggtttggat cttcggatgt
     2701 tgccttatgg ctgtttagca acaggagatc gctctggcct cattgaagtt gtgagcacct
     2761 ctgaaacaat tgctgacatt cagctgaaca gtagcaatgt ggctgctgca gcagccttca
     2821 acaaagatgc ccttctgaac tggcttaaag aatacaactc tggggatgac ctggaccgag
     2881 ccattgagga atttacactg tcctgtgctg gctactgtgt agcttcttat gtccttggga
     2941 ttggtgacag acatagtgac aacatcatgg tcaaaaaaac tggccagctc ttccacattg
     3001 actttggaca tattcttgga aatttcaaat ctaagtttgg cattaaaagg gagcgagtgc
     3061 cttttattct tacctatgat ttcatccatg tcattcaaca aggaaaaaca ggaaatacag
     3121 aaaagtttgg ccggttccgc cagtgttgtg aggatgcata tctgatttta cgacggcatg
     3181 ggaatctctt catcactctc tttgcgctga tgttgactgc agggcttcct gaactcacat
     3241 cagtcaaaga tatacagtat cttaaggact ctcttgcatt agggaagagt gaagaagaag
     3301 cactcaaaca gtttaagcaa aaatttgatg aggcgctcag ggaaagctgg actactaaag
     3361 tgaactggat ggcccacaca gttcggaaag actacagatc ttgattgcca actttcttgt
     3421 acaaagttgg cattataaga aagcattgct tatcaatttg ttgcaacgaa caggtcacta
     3481 tcagtcaaaa taaaatcatt atttgccatc cagctgatat cccctatagt gagtcgtatt
     3541 acatggtcat agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca
     3601 ttgcacaaga taaaaatata tcatcatgcc tcctctagac cagccaggac agaaatgcct
     3661 cgacttcgct gctacccaag gttgccgggt gacgcacacc gtggaaacgg atgaaggcac
     3721 gaacccagtg gacataagcc tgttcggttc gtaagctgta atgcaagtag cgtatgcgct
     3781 cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca gtggcggttt
     3841 tcatggcttg ttatgactgt ttttttgggg tacagtctat gcctcgggca tccaagcagc
     3901 aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat gttacgcagc
     3961 agggcagtcg ccctaaaaca aagttaaaca ttatgaggga agcggtgatc gccgaagtat
     4021 cgactcaact atcagaggta gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg
     4081 ccgtacattt gtacggctcc gcagtggatg gcggcctgaa gccacacagt gatattgatt
     4141 tgctggttac ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg atcaacgacc
     4201 ttttggaaac ttcggcttcc cctggagaga gcgagattct ccgcgctgta gaagtcacca
     4261 ttgttgtgca cgacgacatc attccgtggc gttatccagc taagcgcgaa ctgcaatttg
     4321 gagaatggca gcgcaatgac attcttgcag gtatcttcga gccagccacg atcgacattg
     4381 atctggctat cttgctgaca aaagcaagag aacatagcgt tgccttggta ggtccagcgg
     4441 cggaggaact ctttgatccg gttcctgaac aggatctatt tgaggcgcta aatgaaacct
     4501 taacgctatg gaactcgccg cccgactggg ctggcgatga gcgaaatgta gtgcttacgt
     4561 tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg
     4621 actgggcaat ggagcgcctg ccggcccagt atcagcccgt catacttgaa gctagacagg
     4681 cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg
     4741 tccactacgt gaaaggcgag atcaccaagg tagtcggcaa ataaccctcg agccacccat
     4801 gaccaaaatc ccttaacgtg agttacgcgt cgttccactg agcgtcagac cccgtagaaa
     4861 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     4921 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     4981 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     5041 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     5101 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     5161 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     5221 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     5281 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     5341 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     5401 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     5461 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     5521 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     5581 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     5641 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     5701 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatacgc
     5761 gtaccgctag ccaggaagag tttgtagaaa cgcaaaaagg ccatccgtca ggatggcctt
     5821 ctgcttagtt tgatgcctgg cagtttatgg cgggcgtcct gcccgccacc ctccgggccg
     5881 ttgcttcaca acgttcaaat ccgctcccgg cggatttgtc ctactcagga gagcgttcac
     5941 cgacaaacaa cagataaaac gaaaggccca gtcttccgac tgagcctttc gttttatttg
     6001 atgcct
//
