LOCUS       sequence-16711-e        1395 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1395)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1997
REFERENCE   2  (bases 1 to 1395)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: L3609
FEATURES             Location/Qualifiers
     source          1..1395
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             476..496
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(539..567)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 gngttacatg atcttactaa ctaactattc tcatttaaat tttcagagct taaaaatggc
       61 tgaaatcact cacaacgatg gatacgctaa caacttggaa atgaaataag cttgcatgcc
      121 tgcaggtcga ctctagagga tccccaattc ttgagacttc cactatcttc tctggcaccc
      181 tccactacgc gtaggcgtct ctcgcttcgt gtattcccgg gaagccgggt tcccgtctct
      241 cccgccgctg ccgctgccgc acacagcttt acacctcgta gaatccccaa agaggggcgt
      301 ggcttgcggg tgccaacatc ctcctgccga ggaagaagca ggcactcatc actcgcatca
      361 tcaacctcgg gattggccaa aggacccaaa ggtatgtttc gaatgatact aacataacat
      421 agaacatttt caggaggacc cttggagggt accgagctca gaaaaaatga ctgctccaaa
      481 gaagaagcgt aaggtaccgg tagaaaaaat gagtaaagga gaagaacttt tcactggagt
      541 tgtcccaatt cttgttgaat tagatggtga tgttaatggg cacaaatttt ctgtcagtgg
      601 agagggtgaa ggtgatgcaa catacggaaa acttaccctt aaatttattt gcactactgg
      661 aaaactacct gttccatggg taagtttaaa catatatata ctaactaacc ctgattattt
      721 aaattttcag ccaacacttg tcactacttt cacttatggt gttcaatgct tctcgagata
      781 cccagatcat atgaaacagc atgacttttt caagagtgcc atgcccgaag gttatgtaca
      841 ggaaagaact atatttttca agatgacggg aactacaaga cacgtaagtt taaacagttc
      901 ggtactaact aaccatacat atttaaattt tcaggtgctg aagtcaagtt tgaaggtgat
      961 acccnttgnt taatagaatc gagttaaaag gtatngattt tnaagaagat ggaaacnttc
     1021 ttgganacaa attggaatac aacttcaact cncacaacgt atacttcgca gcaaaacaaa
     1081 caaaagaang gaatcaagtt gtaagtttna acatgatttn ncanctacta atcngattta
     1141 atttccnaac ttcnaaataa accnncttng aaanggagcg ttcactngcn accttttcac
     1201 aanncncnat ggggaggcnt ntcttttaca aaaacttnct nnccaaaatt gnctttnaaa
     1261 ntcnacaaaa aaaccnggcc tttgattnta accngggana ccggggggga aaaaaaacnt
     1321 cgaaggngtt anttccacca cncctttntt naaacagggc nccctnattg ggaaaagncc
     1381 ccaaaaaaat nanaa
//
