LOCUS       sequence-167115-        1012 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses PCNA3 variant (R112C/T180C) fused to P450 BM3 variant 
            (A74G/C773S/C810S) in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Tan CY, Hirakawa H, Suzuki R, Haga T, Iwata F, Nagamune T
  TITLE     Immobilization of a Bacterial Cytochrome P450 Monooxygenase System 
            on a Solid Support.
  JOURNAL   Angew Chem Int Ed Engl. 2016 Oct 26. doi: 10.1002/anie.201608033.
  PUBMED    27781345
REFERENCE   2  (bases 1 to 1012)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Angew Chem 
            Int Ed Engl. 2016 Oct 26. doi: 10.1002/anie.201608033."
COMMENT     Sequence Label: p3R112C/T180CBM3
FEATURES             Location/Qualifiers
     source          1..1012
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             18..40
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             59..76
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             86..103
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 tcccctctag aaataatttt gtttaacttt aagaaggaga tataccatgg gcagcagcca
       61 tcatcatcat catcacagca gcggcctggt gccgcgcggc agccatatga tctacctgaa
      121 atctttcgaa cgtaacatcc gcctgatcaa tatgaaagtg gtttacgatg atgttcgtgt
      181 gctgaaagat attatccagg cgctggcccg cctggttgat gaagccgtgc tgaaattcaa
      241 acaggatagc gttgaactgg tggcactgga tcgtgcgcat attagcctga tctctgttaa
      301 cctgccgcgc gaaatgttca aagaatacga tgtgaacgat gaatttaaat tcggtttcaa
      361 cacccagtac ctgatgaaaa tcctgaaagt tgcaaaacgt aaagaagcca ttgaaatcgc
      421 aagtgaaagc ccggatagtg tgattatcaa cattatcggc agcaccaatc gtgaatttaa
      481 cgtttgcaat ctggaagtgt ctgaacagga aattccggaa atcaacctgc agttcgatat
      541 tagtgcgacg atcagctctg atggttttaa aagtgccatc agcgaagttt ctaccgtgac
      601 ggataatgtg gttgtggaag gtcacgaaga tcgcattctg atcaaagcag aaggcgaaag
      661 cgaagttgaa gtggaatttt ctaaagattg cggcggtctg caggatctgg aatttagtaa
      721 agaaagcaaa aattcttaca gtgcggaata cctggatgat gttctgtctc tgacgaaact
      781 gagtgattac gtgaaaatca gcttcggcaa ccagaaaccg ctgcagctgt ttttcaatat
      841 ggaaggcggt ggcaaagtta cgtacctgct ggcgccgaaa gtgggaggta gcatgacaat
      901 taaagaaatg cctcagccaa aaacgtttgg agagcttaaa aatttaccgt tattaaacac
      961 agataaaccg gttcaagctt tgatgaaaat tgcggatgaa ttaggagaaa tc
//
