LOCUS       sequence-167116-       10010 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  homology donor for AAT locus with piggyBac-flanked 
            DsRedEx_T2A_puroTK cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10010)
  AUTHORS   Eggenschwiler R, Moslem M, Fraguas MS, Galla M, Papp O, Naujock M, 
            Fonfara I, Gensch I, Wahner A, Beh-Pajooh A, Mussolino C, Tauscher 
            M, Steinemann D, Wegner F, Petri S, Schambach A, Charpentier E, 
            Cathomen T, Cantz T
  TITLE     Improved bi-allelic modification of a transcriptionally silent locus
            in patient-derived iPSC by Cas9 nickase.
  JOURNAL   Sci Rep. 2016 Dec 2;6:38198. doi: 10.1038/srep38198.
  PUBMED    27910942
REFERENCE   2  (bases 1 to 10010)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-12-2"; volume: "6:38198. doi"; pages: " 
            10.1038/srep38198"
COMMENT     Sequence Label: AAT-PB-CD2APtk
FEATURES             Location/Qualifiers
     source          1..10010
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   1680..1714
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        2004..2383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2386..2661
                     /label=chicken beta-actin promoter
     intron          2662..3674
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     3598..3620
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     3682..3701
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      3746..3755
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3752..4426
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH
                     LFL"
     primer_bind     complement(3882..3900)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(3932..3952)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     4150..4169
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     4344..4367
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     CDS             4442..4495
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4499..5095
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4499..4518)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4995..5015
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             5102..6097
                     /codon_start=1
                     /product="truncated herpes simplex virus thymidine kinase 
                     (Salomon et al., 1995)"
                     /label=Delta-TK
                     /note="confers sensitivity to nucleoside analogs such as 
                     acyclovir or ganciclovir"
                     /translation="MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWQV
                     LGASETIANIYTTQHRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGS
                     SHAPPPALTLIFDRHPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGAL
                     PEDRHIDRLAKRQRPGERLDLAMLAAIRRVYGLLANTVRYLQGGGSWREDWGQLSGTAV
                     PPQGAEPQSNAGPRPHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRPMHVFI
                     LDYDQSPAGCRDALLQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
     primer_bind     complement(6119..6136)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6125..6349
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    complement(6435..6459)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     repeat_region   6641..6703
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     7726..7742
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7776..7792)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7777..7793)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(7822..7842)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7822..7840)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7861..7877)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7861..7877)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7874..7896)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7885..7901
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7909..7915,7916..7933,7934..7939))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7954..7975
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8092..8109)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8263..8851)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8343..8362)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9022..9813,9814..9882))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9645..9664
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9883..9987)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcgt
      661 ttaaacggcg cgcctctgca cgacaggtct gccagcttac atttacccaa actgtccatt
      721 actggaacct atgatctgaa gagcgtcctg ggtcaactgg gcatcactaa ggtcttcagc
      781 aatggggctg acctctccgg ggtcacagag gaggcacccc tgaagctctc caaggtgaga
      841 tcaccctgac gaccttgttg caccctggta tctgtaggga agaatgtgtg ggggctgcag
      901 ctctgtcctg aggctgagga aggggccgag ggaaacaaat gaagacccag gctgagctcc
      961 tgaagatgcc cgtgattcac tgacacggga cgtggtcaaa cagcaaagcc aggcagggga
     1021 ctgctgtgca gctggcactt tcggggcctc ccttgaggtt gtgtcactga ccctgaattt
     1081 caactttgcc caagaccttc tagacattgg gccttgattt atccatactg acacagaaag
     1141 gtttgggcta agttgtttca aaggaatttc tgactccttc gatctgtgag atttggtgtc
     1201 tgaattaatg aatgatttca gctaaagatg acacttattt tggaaaacta aaggcgacca
     1261 atgaacaact gcagttccat gaatggctgc attatcttgg ggtctgggca ctgtgaaggt
     1321 cactgccagg gtccgtgtcc tcaaggagct tcaagccgtg tactagaaag gagagagccc
     1381 tggaggcaga cgtggagtga cgatgctctt ccctgttctg agttgtgggt gcacctgagc
     1441 agggggagag gcgcttgtca ggaagatgga cagaggggag ccagccccat cagccaaagc
     1501 cttgaggagg agcaaggcct atgtgacagg gagggagagg atgtgcaggg ccagggccgt
     1561 ccagggggag tgagcgcttc ctgggaggtg tccacgtgag ccttgctcga ggcctgggat
     1621 cagccttaca acgtgtctct gcttctctcc cctccaggcc gtgcataagg ctgtgttaac
     1681 cctagaaaga tagtctgcgt aaaattgacg catgcattct tgaaatattg ctctctcttt
     1741 ctaaatagcg cgaatccgtc gctgtgcatt taggacatct cagtcgccgc ttggagctcc
     1801 cgtgaggcgt gcttgtcaat gcggtaagtg tcactgattt tgaactataa cgaccgcgtg
     1861 agtcaaaatg acgcatgatt atcttttacg tgacttttaa gatttaactc atacgataat
     1921 tatattgtta tttcatgttc tacttacgtg ataacttatt atatatatat tttcttgtta
     1981 tagatatcaa ctagaatgct agcgacattg attattgact agttattaat agtaatcaat
     2041 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa
     2101 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
     2161 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
     2221 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
     2281 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
     2341 tacttggcag tacatctacg tattagtcat cgctattacc atgggtcgag gtgagcccca
     2401 cgttctgctt cactctcccc atctcccccc cctccccacc cccaattttg tatttattta
     2461 ttttttaatt attttgtgca gcgatggggg cggggggggg gggggcgcgc gccaggcggg
     2521 gcggggcggg gcgaggggcg gggcggggcg aggcggagag gtgcggcggc agccaatcag
     2581 agcggcgcgc tccgaaagtt tccttttatg gcgaggcggc ggcggcggcg gccctataaa
     2641 aagcgaagcg cgcggcgggc gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc
     2701 gccgcctcgc gccgcccgcc ccggctctga ctgaccgcgt tactcccaca ggtgagcggg
     2761 cgggacggcc cttctcctcc gggctgtaat tagcgcttgg tttaatgacg gctcgtttct
     2821 tttctgtggc tgcgtgaaag ccttaaaggg ctccgggagg gccctttgtg cgggggggag
     2881 cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg
     2941 cccggcggct gtgagcgctg cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg
     3001 aggggagcgc ggccgggggc ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg
     3061 ctgcgtgcgg ggtgtgtgcg tgggggggtg agcagggggt gtgggcgcgg cggtcgggct
     3121 gtaacccccc cctgcacccc cctccccgag ttgctgagca cggcccggct tcgggtgcgg
     3181 ggctccgtac ggggcgtggc gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg
     3241 gggtgccggg cggggcgggg ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg
     3301 gcccccggag cgccggcggc tgtcgaggcg cggcgagccg cagccattgc cttttatggt
     3361 aatcgtgcga gagggcgcag ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg
     3421 ggaggcgccg ccgcaccccc tctagcgggc gcggggcgaa gcggtgcggc gccggcagga
     3481 aggaaatggg cggggagggc cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc
     3541 agcctcgggg ctgtccgcgg ggggacggct gccttcgggg gggacggggc agggcggggt
     3601 tcggcttctg gcgtgtgacc ggcggctcta gagcctctgc taaccatgtt catgccttct
     3661 tctttttcct acagctcctg ggcaacgtgc tggttattgt gctgtctcat cattttggca
     3721 aagaattcct cgagatccac cggtcgccac catggcctcc tccgaggacg tcatcaagga
     3781 gttcatgcgc ttcaaggtgc gcatggaggg ctccgtgaac ggccacgagt tcgagatcga
     3841 gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa
     3901 gggcggcccc ctgcccttcg cctgggacat cctgtccccc cagttccagt acggctccaa
     3961 ggtgtacgtg aagcaccccg ccgacatccc cgactacaag aagctgtcct tccccgaggg
     4021 cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga
     4081 ctcctccctg caggacggct ccttcatcta caaggtgaag ttcatcggcg tgaacttccc
     4141 ctccgacggc cccgtaatgc agaagaagac tatgggctgg gaggcctcca ccgagcgcct
     4201 gtacccccgc gacggcgtgc tgaagggcga gatccacaag gccctgaagc tgaaggacgg
     4261 cggccactac ctcgtggagt tcaagtccat ctacatggcc aagaagcccg tgcagctgcc
     4321 cggctactac tacgtggact ccaagctgga catcacctcc cacaacgagg actacaccat
     4381 cgtggagcag tacgagcgcg ccgagggccg ccaccacctg ttcctgtaca agggctccgg
     4441 agagggccgg ggctctctgc tgacctgtgg cgacgtggag gagaaccccg gcccctccat
     4501 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg
     4561 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg
     4621 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
     4681 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
     4741 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
     4801 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
     4861 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
     4921 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
     4981 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
     5041 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgccggatc
     5101 catgcccacg ctactgcggg tttatataga cggtcctcac gggatgggga aaaccaccac
     5161 cacgcaactg ctggtggccc tgggttcgcg cgacgatatc gtctacgtac ccgagccgat
     5221 gacttactgg caggtgctgg gggcttccga gacaatcgcg aacatctaca ccacacaaca
     5281 ccgcctcgac cagggtgaga tatcggccgg ggacgcggcg gtggtaatga caagcgccca
     5341 gataacaatg ggcatgcctt atgccgtgac cgacgccgtt ctggctcctc atatcggggg
     5401 ggaggctggg agctcacatg ccccgccccc ggccctcacc ctcatcttcg accgccatcc
     5461 catcgccgcc ctcctgtgct acccggccgc gcgatacctt atgggcagca tgacccccca
     5521 ggccgtgctg gcgttcgtgg ccctcatccc gccgaccttg cccggcacaa acatcgtgtt
     5581 gggggccctt ccggaggaca gacacatcga ccgcctggcc aaacgccagc gccccggcga
     5641 gcggcttgac ctggctatgc tggccgcgat tcgccgcgtt tacgggctgc ttgccaatac
     5701 ggtgcggtat ctgcagggcg gcgggtcgtg gcgggaggat tggggacagc tttcggggac
     5761 ggccgtgccg ccccagggtg ccgagcccca gagcaacgcg ggcccacgac cccatatcgg
     5821 ggacacgtta tttaccctgt ttcgggcccc cgagttgctg gcccccaacg gcgacctgta
     5881 caacgtgttt gcctgggcct tggacgtctt ggccaaacgc ctccgtccca tgcacgtctt
     5941 tatcctggat tacgaccaat cgcccgccgg ctgccgggac gccctgctgc aacttacctc
     6001 cgggatggtc cagacccacg tcaccacccc cggctccata ccgacgatct gcgacctggc
     6061 gcgcacgttt gcccgggaga tgggggaggc taactgagct ctagagctcg ctgatcagcc
     6121 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     6181 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
     6241 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
     6301 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg
     6361 gaaagaacca gctggggctc gagatccact agttctagcc tcgaggctag agcggccgca
     6421 ctcgagatat ctagacccag ctttcttgta caaagtggta ctagttaaaa gttttgttac
     6481 tttatagaag aaattttgag tttttgtttt tttttaataa ataaataaac ataaataaat
     6541 tgtttgttga atttattatt agtatgtaag tgtaaatata ataaaactta atatctattc
     6601 aaattaataa ataaacctcg atatacagac cgataaaaca catgcgtcaa ttttacgcat
     6661 gattatcttt aacgtacgtc acaatatgat tatctttcta gggttaacca tcgacgagaa
     6721 agggactgaa gctgctgggg ccatgttttt agaggccata cccatgtcta tcccccccga
     6781 ggtcaagttc aacaaaccct ttgtcttctt aatgattgaa caaaatacca agtctcccct
     6841 cttcatggga aaagtggtga atcccaccca aaaataactg cctctcgctc ctcaacccct
     6901 cccctccatc cctggccccc tccctggatg acattaaaga agggttgagc tggtccctgc
     6961 ctgcatgtga ctgtaaatcc ctcccatgtt ttctctgagt ctccctttgc ctgctgaggc
     7021 tgtatgtggg ctccaggtaa cagtgctgtc ttcgggcccc ctgaactgtg ttcatggagc
     7081 atctggctgg gtaggcacat gctgggcttg aatccagggg ggactgaatc ctcagcttac
     7141 ggacctgggc ccatctgttt ctggagggct ccagtcttcc ttgtcctgtc ttggagtccc
     7201 caagaaggaa tcacagggga ggaaccagat accagccatg accccaggct ccaccaagca
     7261 tcttcatgtc cccctgctca tcccccactc ccccccaccc agagttgctc atcctgccag
     7321 ggctggctgt gcccacccca aggctgccct cctgggggcc ccagaactgc ctgatcgtgc
     7381 cgtggcccag ttttgtggca tctgcagcaa cacaagagag aggacaatgt cctcctcttg
     7441 acccgctgtc acctaaccag actcgggccc tgcacctctc aggcacttct ggaaaatgac
     7501 tgaggcagat tcttcctgaa gcccattctc catggggcaa caaggacacc tattctgtcc
     7561 ttgtccttcc atcgctgccc cagaaagcct cacatatctc cgtttagaat caggtccctt
     7621 ctccccagat gaagaggagg gtctctgctt tgttttctct atctcctcct cagacttgac
     7681 caggcccagc aggccccaga agaccattaa ttaaggccgg gccgctctag aactagtgga
     7741 tcccccgggc tgcaggaatt cgatatcaag cttatcgata ccgtcgacct cgaggggggg
     7801 cccggtaccc agcttttgtt ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg
     7861 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     7921 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     7981 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     8041 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     8101 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     8161 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     8221 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     8281 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     8341 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     8401 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     8461 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     8521 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     8581 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     8641 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     8701 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     8761 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     8821 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     8881 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     8941 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     9001 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     9061 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     9121 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     9181 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     9241 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     9301 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     9361 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     9421 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     9481 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     9541 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     9601 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     9661 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     9721 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     9781 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     9841 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     9901 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     9961 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
//
