LOCUS       sequence-167117-         993 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses PCNA3 variant (R112C/T180C) fused to P450 BM3 variant 
            (A74G/C773S/C810S) in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 993)
  AUTHORS   Tan CY, Hirakawa H, Suzuki R, Haga T, Iwata F, Nagamune T
  TITLE     Immobilization of a Bacterial Cytochrome P450 Monooxygenase System 
            on a Solid Support.
  JOURNAL   Angew Chem Int Ed Engl. 2016 Oct 26. doi: 10.1002/anie.201608033.
  PUBMED    27781345
REFERENCE   2  (bases 1 to 993)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Angew Chem 
            Int Ed Engl. 2016 Oct 26. doi: 10.1002/anie.201608033."
COMMENT     Sequence Label: p3R112C/T180CBM3
FEATURES             Location/Qualifiers
     source          1..993
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(35..52)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cttcctttcg ggctttgtta gcagccggat ctcagtggtg gtggtggtgg tgctcgagtg
       61 cggccgcaag cttgtcgacg gagctcgaat tcggatcctt acccagccca cacgtctttt
      121 gcgtatcggc ctttttcttc tagctgctgc agccataagc gagcgtctgc ttcactcact
      181 tggtgaacgt cagcatagct tttcataagc gttgcttcaa cggcaggtgc catttggctt
      241 ccgtctccgc aaatatagaa gtgcgctcct tgatcaagaa gttcaatcaa tttcttgccg
      301 tcttgttcca ttacgtgctg aacgtatgtt ttcggctgat ttggcatgcg agaaaaagcg
      361 gtatgaagcg taatgatgcc ttcgctttgg gcgttttcaa gctcttcttg atacagatag
      421 tcttcatgag gtgaacggca gccgaagtat aaatgtgctt ctccaagtga ctgtccttgt
      481 tcttttagct gtttgcgcgc ctgcacaaag cctctaaacg gcgcgacgcc tgttcccggt
      541 ccgaccatga taagcggcgt ttcagggtct tttggcagcg taaattctga ctgcggtgtg
      601 gaaataaagc acgtaatcgt atctccttct tgcagctcgg caagatagtt cgacgcaatt
      661 cctttatatt ctccatatcc gctccacgct tctcctgaga caacgctgac cgtgatgctt
      721 gcttgttttt catcgacacg aggtgatgaa gaaatcgagt aatagcgcgg gcgtatgctt
      781 ggcagaaggg cgataaattc gctgaatttc atttcgctcg ccgggtattt ttcaagcagt
      841 tcaagcattg ttaaacgttt tgccagcact tgttctttgt aggcttgctt ttcaagcaag
      901 gcttcaagct ctactttatg cggcgggctg accgttttag cagccattgc gcgaagctgc
      961 gtgcgcgtaa caggatcttg aagctccacg tat
//
