LOCUS       sequence-16712-e        1371 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1371)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1997
REFERENCE   2  (bases 1 to 1371)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: L3610
FEATURES             Location/Qualifiers
     source          1..1371
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             474..494
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(537..565)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 gngttactga tcttctaact aactattctc atttaaattt tcagagctta aaaatggctg
       61 aaatcactca caacgatgga tacgctaaca acttggaaat gaaataagct tgcatgcctg
      121 caggtcgact ctagaggatc cccaattctt gagacttcca ctatcttctc tggcaccctc
      181 cactacgcgt aggcgtctct cgcttcgtgt attcccggga agccgggttc ccgtctctcc
      241 cgccgctgcc gctgccgcac acagctttac acctcgtaga atccccaaag aggggcgtgg
      301 cttgcgggtg ccaacatcct cctgccgagg aagaagcagg cactcatcac tcgcatcatc
      361 aacctcggga ttggccaaag gacccaaagg tatgtttcga atgatactaa cataacatag
      421 aacattttca ggaggaccct tggagggtac cgagctcaga aaaaatgact gctccaaaga
      481 agaagcgtaa ggtaccggta gaaaaaatga gtaaaggaga agaacttttc actggagttg
      541 tcccaattct tgttgaatta gatggtgatg ttaatgggca caaattttct gtcagtggag
      601 agggtgaagg tgatgcaaca tacggaaaac ttacccttaa atttatttgc actactggaa
      661 aactacctgt tccatgggta agtttaaaca tatatatact aactaaccct gattatttaa
      721 attttcagcc aacacttgtc actactttca cttatggtgt tcaatgcttc tcgagatacc
      781 cagatcatat gaaacagcat gactttttca agagtgccat gcccgaaggt tatgtacagg
      841 aaagaactat atttttcaaa gatgacggga actacaagac acgtaagttt aaacagttcg
      901 gtactaacta accatacata ttttaatttt caggtgctga agtcangttt tgaangtgat
      961 acccttgtta atagaatcga gttaaaaggt attgatttta agaaaatggg aaacattctt
     1021 ggaccnaaat ggaatacaac ttcactccac aacgtntacn tcactgnccg anaaaaaaaa
     1081 aatggggatc aaggggtaag tttaacatga ttttactanc tanctaatct gattnaattt
     1141 nccnanctnc aaattanacc cacntngnaa ngggagcgtn cacttnccna anttttncac
     1201 aaatctccat tgggganggc ctgtcttttn ncaaaaacan tntctgtccc aaaattgcct
     1261 tttaaaannc caaaaaaaaa anccnnggnc cttttgattt nancccnngg aaaccggggg
     1321 gggaaataaa anctncgaaa ggggttacnt tcnccanaac cccctttntt g
//
