LOCUS       sequence-167122-        1063 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses PCNA3 variant (R112C/T180C) fused to heme-FMN domain of 
            P450 BM3 variant (A74G) in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Tan CY, Hirakawa H, Suzuki R, Haga T, Iwata F, Nagamune T
  TITLE     Immobilization of a Bacterial Cytochrome P450 Monooxygenase System 
            on a Solid Support.
  JOURNAL   Angew Chem Int Ed Engl. 2016 Oct 26. doi: 10.1002/anie.201608033.
  PUBMED    27781345
REFERENCE   2  (bases 1 to 1063)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Angew Chem 
            Int Ed Engl. 2016 Oct 26. doi: 10.1002/anie.201608033."
COMMENT     Sequence Label: p3R112C/T180CHFm
FEATURES             Location/Qualifiers
     source          1..1063
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(35..52)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cttcctttcg ggctttgtta gcagccggat ctcagtggtg gtggtggtgg tgctcgagtg
       61 cggccgcaag cttgtcgacg gagctcgaat tcggatcctc aatccgcggc gctgtcgaca
      121 aattgaagtg aaagagtaga tttattatct tcactgtttt caatgtcgag gttaaagtag
      181 gctgctacgt cactccacat atgttcacgc cattcttcat atgtgccttc aaagtcgtcg
      241 cttgcatctg cttcaccgcg gtcagcgatg ttttctgccc ctttagcggc aagcgtttca
      301 tcgataaaag caggcacttt ttgatacgta gtagcccagt ttttatcgcc gcatccaaat
      361 acggagtagc gaacgccttt tacttcatca gcagacgctt ggtctaacca gtcgacaaat
      421 tgctttgcgt tatcaggcgg atgaccgtta taagacgccg ttacaattaa tacagctcct
      481 tcgcgcggaa gatttccggc gtgtgaatca agcgttgcga cctgcggtgc aaatcctttg
      541 ctcattgcaa tatctgctaa atcacgcgcc gttccttcag ctgttcccat atttgaaccg
      601 tatagcacaa gcagcggcgt attatgagcg ttttctgcct ttttgcgtac ttttttagca
      661 gactgttcag tgctaggtga aggaataccg ccaagcggaa tttttttcga ttttgctttt
      721 accacaaagc cttcaggttt taacgttaaa gtttctttaa tatccagctc gtagtttgta
      781 tgatcttcaa agtcaaagtg ttttagcatc ataccaagta ccagcgttgc ttcatgaaga
      841 gcgaactgct gaccgataca cgcacgctga ccgtttccaa acggtttaaa cgcatgctgc
      901 ggaatcgcac ttggattttc aaaacgctct ggacggaact cttccacatc gtctccccaa
      961 attgttttat cacggtgaag ctgaggaatc agaaccatta gttcgtcgcc tttttctaaa
     1021 ggatattctc ctccaagcac cgtatcttct tttgcatata ggg
//
