LOCUS       sequence-167124-       10046 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  homology donor for AAT locus with piggyBac-flanked eGFP_T2A_puroTK 
            cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10046)
  AUTHORS   Eggenschwiler R, Moslem M, Fraguas MS, Galla M, Papp O, Naujock M, 
            Fonfara I, Gensch I, Wahner A, Beh-Pajooh A, Mussolino C, Tauscher 
            M, Steinemann D, Wegner F, Petri S, Schambach A, Charpentier E, 
            Cathomen T, Cantz T
  TITLE     Improved bi-allelic modification of a transcriptionally silent locus
            in patient-derived iPSC by Cas9 nickase.
  JOURNAL   Sci Rep. 2016 Dec 2;6:38198. doi: 10.1038/srep38198.
  PUBMED    27910942
REFERENCE   2  (bases 1 to 10046)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-12-2"; volume: "6:38198. doi"; pages: " 
            10.1038/srep38198"
COMMENT     Sequence Label: AAT-PB-CG2APtk
FEATURES             Location/Qualifiers
     source          1..10046
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   1680..1714
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        2004..2383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2386..2661
                     /label=chicken beta-actin promoter
     intron          2662..3674
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     3598..3620
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     3682..3701
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      3746..3755
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3752..3754,3755..3757,3758..4468)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3752..3754,3755..3757,3758..4468)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3797..3818)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4058..4077)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             4478..4531
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4535..5131
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4535..4554)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5031..5051
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             5138..6133
                     /codon_start=1
                     /product="truncated herpes simplex virus thymidine kinase 
                     (Salomon et al., 1995)"
                     /label=Delta-TK
                     /note="confers sensitivity to nucleoside analogs such as 
                     acyclovir or ganciclovir"
                     /translation="MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWQV
                     LGASETIANIYTTQHRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGS
                     SHAPPPALTLIFDRHPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGAL
                     PEDRHIDRLAKRQRPGERLDLAMLAAIRRVYGLLANTVRYLQGGGSWREDWGQLSGTAV
                     PPQGAEPQSNAGPRPHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRPMHVFI
                     LDYDQSPAGCRDALLQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
     primer_bind     complement(6155..6172)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6161..6385
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    complement(6471..6495)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     repeat_region   6677..6739
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     7762..7778
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7812..7828)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7813..7829)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(7858..7878)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7858..7876)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7897..7913)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7897..7913)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7910..7932)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7921..7937
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7945..7951,7952..7969,7970..7975))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7990..8011
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8128..8145)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8299..8887)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8379..8398)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9058..9849,9850..9918))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9681..9700
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9919..10023)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcgt
      661 ttaaacggcg cgcctctgca cgacaggtct gccagcttac atttacccaa actgtccatt
      721 actggaacct atgatctgaa gagcgtcctg ggtcaactgg gcatcactaa ggtcttcagc
      781 aatggggctg acctctccgg ggtcacagag gaggcacccc tgaagctctc caaggtgaga
      841 tcaccctgac gaccttgttg caccctggta tctgtaggga agaatgtgtg ggggctgcag
      901 ctctgtcctg aggctgagga aggggccgag ggaaacaaat gaagacccag gctgagctcc
      961 tgaagatgcc cgtgattcac tgacacggga cgtggtcaaa cagcaaagcc aggcagggga
     1021 ctgctgtgca gctggcactt tcggggcctc ccttgaggtt gtgtcactga ccctgaattt
     1081 caactttgcc caagaccttc tagacattgg gccttgattt atccatactg acacagaaag
     1141 gtttgggcta agttgtttca aaggaatttc tgactccttc gatctgtgag atttggtgtc
     1201 tgaattaatg aatgatttca gctaaagatg acacttattt tggaaaacta aaggcgacca
     1261 atgaacaact gcagttccat gaatggctgc attatcttgg ggtctgggca ctgtgaaggt
     1321 cactgccagg gtccgtgtcc tcaaggagct tcaagccgtg tactagaaag gagagagccc
     1381 tggaggcaga cgtggagtga cgatgctctt ccctgttctg agttgtgggt gcacctgagc
     1441 agggggagag gcgcttgtca ggaagatgga cagaggggag ccagccccat cagccaaagc
     1501 cttgaggagg agcaaggcct atgtgacagg gagggagagg atgtgcaggg ccagggccgt
     1561 ccagggggag tgagcgcttc ctgggaggtg tccacgtgag ccttgctcga ggcctgggat
     1621 cagccttaca acgtgtctct gcttctctcc cctccaggcc gtgcataagg ctgtgttaac
     1681 cctagaaaga tagtctgcgt aaaattgacg catgcattct tgaaatattg ctctctcttt
     1741 ctaaatagcg cgaatccgtc gctgtgcatt taggacatct cagtcgccgc ttggagctcc
     1801 cgtgaggcgt gcttgtcaat gcggtaagtg tcactgattt tgaactataa cgaccgcgtg
     1861 agtcaaaatg acgcatgatt atcttttacg tgacttttaa gatttaactc atacgataat
     1921 tatattgtta tttcatgttc tacttacgtg ataacttatt atatatatat tttcttgtta
     1981 tagatatcaa ctagaatgct agcgacattg attattgact agttattaat agtaatcaat
     2041 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa
     2101 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
     2161 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
     2221 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
     2281 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
     2341 tacttggcag tacatctacg tattagtcat cgctattacc atgggtcgag gtgagcccca
     2401 cgttctgctt cactctcccc atctcccccc cctccccacc cccaattttg tatttattta
     2461 ttttttaatt attttgtgca gcgatggggg cggggggggg gggggcgcgc gccaggcggg
     2521 gcggggcggg gcgaggggcg gggcggggcg aggcggagag gtgcggcggc agccaatcag
     2581 agcggcgcgc tccgaaagtt tccttttatg gcgaggcggc ggcggcggcg gccctataaa
     2641 aagcgaagcg cgcggcgggc gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc
     2701 gccgcctcgc gccgcccgcc ccggctctga ctgaccgcgt tactcccaca ggtgagcggg
     2761 cgggacggcc cttctcctcc gggctgtaat tagcgcttgg tttaatgacg gctcgtttct
     2821 tttctgtggc tgcgtgaaag ccttaaaggg ctccgggagg gccctttgtg cgggggggag
     2881 cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg
     2941 cccggcggct gtgagcgctg cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg
     3001 aggggagcgc ggccgggggc ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg
     3061 ctgcgtgcgg ggtgtgtgcg tgggggggtg agcagggggt gtgggcgcgg cggtcgggct
     3121 gtaacccccc cctgcacccc cctccccgag ttgctgagca cggcccggct tcgggtgcgg
     3181 ggctccgtac ggggcgtggc gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg
     3241 gggtgccggg cggggcgggg ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg
     3301 gcccccggag cgccggcggc tgtcgaggcg cggcgagccg cagccattgc cttttatggt
     3361 aatcgtgcga gagggcgcag ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg
     3421 ggaggcgccg ccgcaccccc tctagcgggc gcggggcgaa gcggtgcggc gccggcagga
     3481 aggaaatggg cggggagggc cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc
     3541 agcctcgggg ctgtccgcgg ggggacggct gccttcgggg gggacggggc agggcggggt
     3601 tcggcttctg gcgtgtgacc ggcggctcta gagcctctgc taaccatgtt catgccttct
     3661 tctttttcct acagctcctg ggcaacgtgc tggttattgt gctgtctcat cattttggca
     3721 aagaattcct cgagatccac cggtcgccac catggtgagc aagggcgagg agctgttcac
     3781 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
     3841 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
     3901 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca
     3961 gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
     4021 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
     4081 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
     4141 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa
     4201 cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca
     4261 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
     4321 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa
     4381 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
     4441 cactctcggc atggacgagc tgtacaaggg ctccggagag ggccggggct ctctgctgac
     4501 ctgtggcgac gtggaggaga accccggccc ctccatgacc gagtacaagc ccacggtgcg
     4561 cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga
     4621 ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct
     4681 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga
     4741 cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc
     4801 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat
     4861 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg
     4921 cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga
     4981 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc
     5041 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg
     5101 cacctggtgc atgacccgca agcccggtgc cggatccatg cccacgctac tgcgggttta
     5161 tatagacggt cctcacggga tggggaaaac caccaccacg caactgctgg tggccctggg
     5221 ttcgcgcgac gatatcgtct acgtacccga gccgatgact tactggcagg tgctgggggc
     5281 ttccgagaca atcgcgaaca tctacaccac acaacaccgc ctcgaccagg gtgagatatc
     5341 ggccggggac gcggcggtgg taatgacaag cgcccagata acaatgggca tgccttatgc
     5401 cgtgaccgac gccgttctgg ctcctcatat cgggggggag gctgggagct cacatgcccc
     5461 gcccccggcc ctcaccctca tcttcgaccg ccatcccatc gccgccctcc tgtgctaccc
     5521 ggccgcgcga taccttatgg gcagcatgac cccccaggcc gtgctggcgt tcgtggccct
     5581 catcccgccg accttgcccg gcacaaacat cgtgttgggg gcccttccgg aggacagaca
     5641 catcgaccgc ctggccaaac gccagcgccc cggcgagcgg cttgacctgg ctatgctggc
     5701 cgcgattcgc cgcgtttacg ggctgcttgc caatacggtg cggtatctgc agggcggcgg
     5761 gtcgtggcgg gaggattggg gacagctttc ggggacggcc gtgccgcccc agggtgccga
     5821 gccccagagc aacgcgggcc cacgacccca tatcggggac acgttattta ccctgtttcg
     5881 ggcccccgag ttgctggccc ccaacggcga cctgtacaac gtgtttgcct gggccttgga
     5941 cgtcttggcc aaacgcctcc gtcccatgca cgtctttatc ctggattacg accaatcgcc
     6001 cgccggctgc cgggacgccc tgctgcaact tacctccggg atggtccaga cccacgtcac
     6061 cacccccggc tccataccga cgatctgcga cctggcgcgc acgtttgccc gggagatggg
     6121 ggaggctaac tgagctctag agctcgctga tcagcctcga ctgtgccttc tagttgccag
     6181 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
     6241 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
     6301 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
     6361 gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctcgaga
     6421 tccactagtt ctagcctcga ggctagagcg gccgcactcg agatatctag acccagcttt
     6481 cttgtacaaa gtggtactag ttaaaagttt tgttacttta tagaagaaat tttgagtttt
     6541 tgtttttttt taataaataa ataaacataa ataaattgtt tgttgaattt attattagta
     6601 tgtaagtgta aatataataa aacttaatat ctattcaaat taataaataa acctcgatat
     6661 acagaccgat aaaacacatg cgtcaatttt acgcatgatt atctttaacg tacgtcacaa
     6721 tatgattatc tttctagggt taaccatcga cgagaaaggg actgaagctg ctggggccat
     6781 gtttttagag gccataccca tgtctatccc ccccgaggtc aagttcaaca aaccctttgt
     6841 cttcttaatg attgaacaaa ataccaagtc tcccctcttc atgggaaaag tggtgaatcc
     6901 cacccaaaaa taactgcctc tcgctcctca acccctcccc tccatccctg gccccctccc
     6961 tggatgacat taaagaaggg ttgagctggt ccctgcctgc atgtgactgt aaatccctcc
     7021 catgttttct ctgagtctcc ctttgcctgc tgaggctgta tgtgggctcc aggtaacagt
     7081 gctgtcttcg ggccccctga actgtgttca tggagcatct ggctgggtag gcacatgctg
     7141 ggcttgaatc caggggggac tgaatcctca gcttacggac ctgggcccat ctgtttctgg
     7201 agggctccag tcttccttgt cctgtcttgg agtccccaag aaggaatcac aggggaggaa
     7261 ccagatacca gccatgaccc caggctccac caagcatctt catgtccccc tgctcatccc
     7321 ccactccccc ccacccagag ttgctcatcc tgccagggct ggctgtgccc accccaaggc
     7381 tgccctcctg ggggccccag aactgcctga tcgtgccgtg gcccagtttt gtggcatctg
     7441 cagcaacaca agagagagga caatgtcctc ctcttgaccc gctgtcacct aaccagactc
     7501 gggccctgca cctctcaggc acttctggaa aatgactgag gcagattctt cctgaagccc
     7561 attctccatg gggcaacaag gacacctatt ctgtccttgt ccttccatcg ctgccccaga
     7621 aagcctcaca tatctccgtt tagaatcagg tcccttctcc ccagatgaag aggagggtct
     7681 ctgctttgtt ttctctatct cctcctcaga cttgaccagg cccagcaggc cccagaagac
     7741 cattaattaa ggccgggccg ctctagaact agtggatccc ccgggctgca ggaattcgat
     7801 atcaagctta tcgataccgt cgacctcgag ggggggcccg gtacccagct tttgttccct
     7861 ttagtgaggg ttaattgcgc gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     7921 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     7981 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     8041 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     8101 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     8161 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     8221 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     8281 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     8341 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     8401 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     8461 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     8521 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     8581 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     8641 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     8701 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     8761 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     8821 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     8881 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     8941 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     9001 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     9061 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     9121 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     9181 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     9241 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     9301 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     9361 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     9421 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     9481 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     9541 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     9601 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     9661 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     9721 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     9781 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     9841 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     9901 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     9961 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
    10021 gcgcacattt ccccgaaaag tgccac
//
