LOCUS       sequence-167126-        1047 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Host vector to fuse a gene at the C-terminus of PCNA1 variant 
            (G108C) .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Tan CY, Hirakawa H, Suzuki R, Haga T, Iwata F, Nagamune T
  TITLE     Immobilization of a Bacterial Cytochrome P450 Monooxygenase System 
            on a Solid Support.
  JOURNAL   Angew Chem Int Ed Engl. 2016 Oct 26. doi: 10.1002/anie.201608033.
  PUBMED    27781345
REFERENCE   2  (bases 1 to 1047)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Angew Chem 
            Int Ed Engl. 2016 Oct 26. doi: 10.1002/anie.201608033."
COMMENT     Sequence Label: p1G108C-GG
FEATURES             Location/Qualifiers
     source          1..1047
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             20..42
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             61..78
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             88..105
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             913..930
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(983..1001)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      997..1044
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 attcccctct agaaataatt ttgtttaact ttaagaagga gatataccat gggcagcagc
       61 catcatcatc atcatcacag cagcggcctg gtgccgcgcg gcagccatat gtttaaaatt
      121 gtgtatccga acgccaaaga tttctttagc ttcatcaaca gcattaccaa cgttacggat
      181 tctatcatcc tgaacttcac cgaagatggc atcttcagtc gtcatctgac ggaagataaa
      241 gtgctgatgg caatcatgcg cattccgaaa gatgttctgt ctgaatacag tattgatagc
      301 ccgacctctg tgaaactgga tgtgagctct gtgaagaaaa ttctgagcaa agcaagtagc
      361 aaaaaagcga cgattgaact gaccgaaacg gattctggcc tgaaaatcat catccgtgat
      421 gaaaaatctt gcgccaaaag taccatctac atcaaagcag aaaaaggcca ggttgaacag
      481 ctgacggaac cgaaagtgaa cctggccgtt aattttacca cggatgaaag cgtgctgaac
      541 gttatcgcgg ccgatgtgac cctggttggt gaagaaatgc gtatcagcac ggaagaagat
      601 aaaatcaaaa ttgaagcggg cgaagaaggt aaacgctatg tggcctttct gatgaaagat
      661 aaaccgctga aagaactgag catcgatacc tctgcctcta gtagctacag cgcagaaatg
      721 ttcaaagatg cggtgaaagg cctgcgtggt tttagtgcgc cgacgatggt gtctttcggt
      781 gaaaatctgc cgatgaaaat tgatgtggaa gcagttagcg gcggtcacat gatcttctgg
      841 attgcgccgc gcctgggagt gagaccggat ccgaattcga gctccgtcga caagcttgcg
      901 gccgcactcg agcaccacca ccaccaccac tgagatccgg ctgctaacaa agcccgaaag
      961 gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct
     1021 aaacgggtct tgaggggttt tttgctg
//
