LOCUS       sequence-167127-         911 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Sensor Plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Mullokandov G, Baccarini A, Ruzo A, Jayaprakash AD, Tung N, Israelow
            B, Evans MJ, Sachidanandam R, Brown BD
  TITLE     High-throughput assessment of microRNA activity and function using 
            microRNA sensor and decoy libraries.
  JOURNAL   Nat Methods. 2012 Jul 1;9(8):840-6. doi: 10.1038/nmeth.2078.
  PUBMED    22751203
REFERENCE   2  (bases 1 to 911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-07-1- 1"; volume: "9"; 
            issue: "8"; pages: "840-6"
COMMENT     Sequence Label: 
            AB.pCCL.sin.cPPT.GFP.miR-301a-3p.sensor.PGK.dNGFR.WPRE
FEATURES             Location/Qualifiers
     source          1..911
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    83..217
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(120..139)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(539..560)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     888..907
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
       61 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataagg
      121 caatagcatc acaaatttca caaataaggc atttttttca ctgcattcta gttttggttt
      181 gtccaaactc atcaatgtat cttatcatgt ctggatctca aatccctcgg aagctgcgcc
      241 tgtcatcgaa ttcctgcagc ccggtgcatg actaagctag taccggtcag tgcaatagta
      301 ttgtcaaagc agtgcaatag tattgtcaaa gcagtgcaat agtattgtca aagcagtgca
      361 atagtattgt caaagcagtg caatagtatt gtcaaagcag tgcaatagta ttgtcaaagg
      421 ctacgtcgac cagtactccc cagttaaggt cggctagctc agttagcctc ccccatctct
      481 cgacgcggcc gctttacttg tacagctcgt ccatgccgag agtgatcccg gcggcggtca
      541 cgaactccag caggaccatg tgatcgcgct tctcgttggg gtctttgctc agggcggact
      601 gggtgctcag gtagtggttg tcgggcagca gcacggggcc gtcgccgatg ggggtgttct
      661 gctggtagtg gtcggcgagc tgcacgctgc cgtcctcgat gttgtggcgg atcttgaagt
      721 tcaccttgat gccgttcttc tgcttgtcgg ccatgatata gacgttgtgg ctgttgtagt
      781 tgtactccag cttgtgcccc angatgttgc cgtcctcctt gaagtcgatg cccttcagct
      841 cgatgcggtt caccagggtg tcgccctcga acttcacctc ggcgcgggtc ttgtagttgc
      901 cgtcgtcctt g
//
