LOCUS       sequence-167134-        5198 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Ebola Virus (Makona) Glycoprotein in CMV driven mammalian expression
            vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5198)
  AUTHORS   Diehl WE, Lin AE, Grubaugh ND, Carvalho LM, Kim K, Kyawe PP, 
            McCauley SM, Donnard E, Kucukural A, McDonel P, Schaffner SF, Garber
            M, Rambaut A, Andersen KG, Sabeti PC, Luban J
  TITLE     Ebola Virus Glycoprotein with Increased Infectivity Dominated the 
            2013-2016 Epidemic.
  JOURNAL   Cell. 2016 Nov 3;167(4):1088-1098.e6. doi: 
            10.1016/j.cell.2016.10.014.
  PUBMED    27814506
REFERENCE   2  (bases 1 to 5198)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.10"; journalName: "Cell"; date: "2016-11-3- 3";
            volume: "167"; issue: "4"; pages: "1088-1098.e6"
COMMENT     Sequence Label: pGL4.23 A82V/T230A 2014 EBOV + Mucin-Like Domain
FEATURES             Location/Qualifiers
     source          1..5198
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        30..409
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     564..584
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        618..649
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      676..685
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    2752..2873
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2763..2782)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2817..2836
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3121..3138)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3292..3880)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3372..3391)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4080..4871,4872..4940))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    5045..5093
                     /note="synthetic polyadenylation signal"
     misc_feature    5107..5198
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     5147..5166
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ggcctaactg gccgccagat atacgcgttg acattgatta ttgactagtt attaatagta
       61 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
      121 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
      181 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
      241 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
      301 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
      361 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
      421 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
      481 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
      541 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt ggggcctcgg
      601 cggccaagct tagacactag agggtatata atggaagctc gacttccagc ttggcaatcc
      661 ggtactgttg gtaaagccac catgggtgtt acaggaatat tgcagttacc tcgtgatcga
      721 ttcaagagga catcattctt tctttgggta attatccttt tccaaagaac attttccatc
      781 ccgcttggag ttatccacaa tagtacatta caggttagtg atgtcgacaa actagtttgt
      841 cgtgacaaac tgtcatccac aaatcaattg agatcagttg gactgaatct cgaggggaat
      901 ggagtggcaa ctgacgtgcc atctgtgact aaaagatggg gcttcaggtc cggtgtccca
      961 ccaaaggtgg tcaattatga agctggtgaa tgggctgaaa actgctacaa tcttgaaatc
     1021 aaaaaacctg acgggagtga gtgtctacca gcagcgccag acgggattcg gggcttcccc
     1081 cggtgccggt atgtgcacaa agtatcagga acgggaccat gtgccggaga ctttgccttc
     1141 cacaaagagg gtgctttctt cctgtatgat cgacttgctt ccacagttat ctaccgagga
     1201 acgactttcg ctgaaggtgt cgttgcattt ctgatactgc cccaagctaa gaaggacttc
     1261 ttcagctcac accccttgag agagccggtc aatgcaacgg aggacccgtc gagtggctat
     1321 tattctacca caattagata tcaggctacc ggttttggaa ctaatgaggc agagtacttg
     1381 ttcgaggttg acaatttgac ctacgtccaa cttgaatcaa gattcacacc acagtttctg
     1441 ctccagctga atgagacaat atatgcaagt gggaagagga gcaacaccac gggaaaacta
     1501 atttggaagg tcaaccccga aattgataca acaatcgggg agtgggcctt ctgggaaact
     1561 aaaaaaaacc tcactagaaa aattcgcagt gaagagttgt ctttcacagc tgtatcaaac
     1621 ggacccaaaa acatcagtgg tcagagtccg gcgcgaactt cttccgaccc agagaccaac
     1681 acaacaaatg aagaccacaa aatcatggct tcagaaaatt cctctgcaat ggttcaagtg
     1741 cacagtcaag gaaggaaagc tgcagtgtcg catctgacaa cccttgccac aatctccacg
     1801 agtcctcaac ctcccacaac caaaacaggt ccggacaaca gcacccataa tacacccgtg
     1861 tataaacttg acatctctga ggcaactcaa gttggacaac atcaccgtag agcagacaac
     1921 gacagcacag cctccgacac tccccccgcc acgaccgcag ccggaccctt aaaagcagag
     1981 aacaccaaca cgagtaagag cgctgactcc ctggacctcg ccaccacgac aagcccccaa
     2041 aactacagcg agactgctgg caacaacaac actcatcacc aagataccgg agaagagagt
     2101 gccagcagcg ggaagctagg cttaattacc aatactattg ctggagtagc aggactgatc
     2161 acaggcggga gaaggactcg aagagaagta attgtcaatg ctcaacccaa atgcaacccc
     2221 aatttacatt actggactac tcaggatgaa ggtgctgcaa tcggattggc ctggatacca
     2281 tatttcgggc cagcagccga aggaatttac acagaggggc taatgcacaa ccaagatggt
     2341 ttaatctgtg ggttgaggca gctggccaac gaaacgactc aagctctcca actgttcctg
     2401 agagccacaa ctgagctgcg aaccttttca atcctcaacc gtaaggcaat tgacttcctg
     2461 ctgcagcgat ggggtggcac atgccacatt ttgggaccgg actgctgtat cgaaccacat
     2521 gattggacca agaacataac agacaaaatt gatcagatta ttcatgattt tgttgataaa
     2581 acccttccgg accaggggga caatgacaat tggtggacag gatggagaca atggataccg
     2641 gcaggtattg gagttacagg tgttataatt gcagttatcg ctttattctg tatatgcaaa
     2701 tttgtctttt agtctagagt cggggcggcc ggccgcttcg agcagacatg ataagataca
     2761 ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa
     2821 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca
     2881 acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca
     2941 agtaaaacct ctacaaatgt ggtaaaatcg ataaggatcc gtcgaccgat gcccttgaga
     3001 gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt cgccgcactt
     3061 atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct cttccgcttc
     3121 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     3181 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     3241 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     3301 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     3361 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     3421 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     3481 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     3541 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     3601 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     3661 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     3721 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     3781 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     3841 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     3901 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     3961 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     4021 aagtatatat gagtaaactt ggtctgacag cggccgcaaa tgctaaacca ctgcagtggt
     4081 taccagtgct tgatcagtga ggcaccgatc tcagcgatct gcctatttcg ttcgtccata
     4141 gtggcctgac tccccgtcgt gtagatcact acgattcgtg agggcttacc atcaggcccc
     4201 agcgcagcaa tgatgccgcg agagccgcgt tcaccggccc ccgatttgtc agcaatgaac
     4261 cagccagcag ggagggccga gcgaagaagt ggtcctgcta ctttgtccgc ctccatccag
     4321 tctatgagct gctgtcgtga tgctagagta agaagttcgc cagtgagtag tttccgaaga
     4381 gttgtggcca ttgctactgg catcgtggta tcacgctcgt cgttcggtat ggcttcgttc
     4441 aactctggtt cccagcggtc aagccgggtc acatgatcac ccatattatg aagaaatgca
     4501 gtcagctcct tagggcctcc gatcgttgtc agaagtaagt tggccgcggt gttgtcgctc
     4561 atggtaatgg cagcactaca caattctctt accgtcatgc catccgtaag atgcttttcc
     4621 gtgaccggcg agtactcaac caagtcgttt tgtgagtagt gtatacggcg accaagctgc
     4681 tcttgcccgg cgtctatacg ggacaacacc gcgccacata gcagtacttt gaaagtgctc
     4741 atcatcggga atcgttcttc ggggcggaaa gactcaagga tcttgccgct attgagatcc
     4801 agttcgatat agcccactct tgcacccagt tgatcttcag catcttttac tttcaccagc
     4861 gtttcggggt gtgcaaaaac aggcaagcaa aatgccgcaa agaagggaat gagtgcgaca
     4921 cgaaaatgtt ggatgctcat actcgtcctt tttcaatatt attgaagcat ttatcagggt
     4981 tactagtacg tctctcaagg ataagtaagt aatattaagg tacgggaggt attggacagg
     5041 ccgcaataaa atatctttat tttcattaca tctgtgtgtt ggttttttgt gtgaatcgat
     5101 agtactaaca tacgctctcc atcaaaacaa aacgaaacaa aacaaactag caaaataggc
     5161 tgtccccagt gcaagtgcag gtgccagaac atttctct
//
