LOCUS       sequence-167144-        1060 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Host vector to fuse a gene at the C-terminus of PCNA3 variant 
            (R112C/T180C).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Tan CY, Hirakawa H, Suzuki R, Haga T, Iwata F, Nagamune T
  TITLE     Immobilization of a Bacterial Cytochrome P450 Monooxygenase System 
            on a Solid Support.
  JOURNAL   Angew Chem Int Ed Engl. 2016 Oct 26. doi: 10.1002/anie.201608033.
  PUBMED    27781345
REFERENCE   2  (bases 1 to 1060)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Angew Chem 
            Int Ed Engl. 2016 Oct 26. doi: 10.1002/anie.201608033."
COMMENT     Sequence Label: p3R112C/T180C-GG
FEATURES             Location/Qualifiers
     source          1..1060
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             20..42
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             61..78
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             88..105
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             943..960
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(1013..1031)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 attcccctct agaaataatt ttgtttaact ttaagaagga gatataccat gggcagcagc
       61 catcatcatc atcatcacag cagcggcctg gtgccgcgcg gcagccatat gatctacctg
      121 aaatctttcg aacgtaacat ccgcctgatc aatatgaaag tggtttacga tgatgttcgt
      181 gtgctgaaag atattatcca ggcgctggcc cgcctggttg atgaagccgt gctgaaattc
      241 aaacaggata gcgttgaact ggtggcactg gatcgtgcgc atattagcct gatctctgtt
      301 aacctgccgc gcgaaatgtt caaagaatac gatgtgaacg atgaatttaa attcggtttc
      361 aacacccagt acctgatgaa aatcctgaaa gttgcaaaac gtaaagaagc cattgaaatc
      421 gcaagtgaaa gcccggatag tgtgattatc aacattatcg gcagcaccaa tcgtgaattt
      481 aacgtttgca atctggaagt gtctgaacag gaaattccgg aaatcaacct gcagttcgat
      541 attagtgcga cgatcagctc tgatggtttt aaaagtgcca tcagcgaagt ttctaccgtg
      601 acggataatg tggttgtgga aggtcacgaa gatcgcattc tgatcaaagc agaaggcgaa
      661 agcgaagttg aagtggaatt ttctaaagat tgcggcggtc tgcaggatct ggaatttagt
      721 aaagaaagca aaaattctta cagtgcggaa tacctggatg atgttctgtc tctgacgaaa
      781 ctgagtgatt acgtgaaaat cagcttcggc aaccagaaac cgctgcagct gtttttcaat
      841 atggaaggcg gtggcaaagt tacgtacctg ctggcgccga aagtgggagt gagaccggat
      901 ccgaattcga gctccgtcga caagcttgcg gccgcactcg agcaccacca ccaccaccac
      961 tgagatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag
     1021 caataactag cataacccct tggggcctct aaacgggtct
//
