LOCUS       sequence-167145-        1054 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Host vector to fuse a gene at the C-terminus of PCNA3 variant 
            (R112C/T180C).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Tan CY, Hirakawa H, Suzuki R, Haga T, Iwata F, Nagamune T
  TITLE     Immobilization of a Bacterial Cytochrome P450 Monooxygenase System 
            on a Solid Support.
  JOURNAL   Angew Chem Int Ed Engl. 2016 Oct 26. doi: 10.1002/anie.201608033.
  PUBMED    27781345
REFERENCE   2  (bases 1 to 1054)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Angew Chem 
            Int Ed Engl. 2016 Oct 26. doi: 10.1002/anie.201608033."
COMMENT     Sequence Label: p3R112C/T180C-GG
FEATURES             Location/Qualifiers
     source          1..1054
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(35..52)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(890..907)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(917..934)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             953..975
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    990..1014
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(1014..1033)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1015..1033)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cttcctttcg ggctttgtta gcagccggat ctcagtggtg gtggtggtgg tgctcgagtg
       61 cggccgcaag cttgtcgacg gagctcgaat tcggatccgg tctcactccc actttcggcg
      121 ccagcaggta cgtaactttg ccaccgcctt ccatattgaa aaacagctgc agcggtttct
      181 ggttgccgaa gctgattttc acgtaatcac tcagtttcgt cagagacaga acatcatcca
      241 ggtattccgc actgtaagaa tttttgcttt ctttactaaa ttccagatcc tgcagaccgc
      301 cgcaatcttt agaaaattcc acttcaactt cgctttcgcc ttctgctttg atcagaatgc
      361 gatcttcgtg accttccaca accacattat ccgtcacggt agaaacttcg ctgatggcac
      421 ttttaaaacc atcagagctg atcgtcgcac taatatcgaa ctgcaggttg atttccggaa
      481 tttcctgttc agacacttcc agattgcaaa cgttaaattc acgattggtg ctgccgataa
      541 tgttgataat cacactatcc gggctttcac ttgcgatttc aatggcttct ttacgttttg
      601 caactttcag gattttcatc aggtactggg tgttgaaacc gaatttaaat tcatcgttca
      661 catcgtattc tttgaacatt tcgcgcggca ggttaacaga gatcaggcta atatgcgcac
      721 gatccagtgc caccagttca acgctatcct gtttgaattt cagcacggct tcatcaacca
      781 ggcgggccag cgcctggata atatctttca gcacacgaac atcatcgtaa accactttca
      841 tattgatcag gcggatgtta cgttcgaaag atttcaggta gatcatatgg ctgccgcgcg
      901 gcaccaggcc gctgctgtga tgatgatgat gatggctgct gcccatggta tatctccttc
      961 ttaaagttaa acaaaattat ttctagaggg gaattgttat ccgctcacaa ttcccctata
     1021 gtgagtcgta ttaatttcgc gggatcgaga tctc
//
