LOCUS       sequence-167146-         925 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Sensor Plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 925)
  AUTHORS   Mullokandov G, Baccarini A, Ruzo A, Jayaprakash AD, Tung N, Israelow
            B, Evans MJ, Sachidanandam R, Brown BD
  TITLE     High-throughput assessment of microRNA activity and function using 
            microRNA sensor and decoy libraries.
  JOURNAL   Nat Methods. 2012 Jul 1;9(8):840-6. doi: 10.1038/nmeth.2078.
  PUBMED    22751203
REFERENCE   2  (bases 1 to 925)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-07-1- 1"; volume: "9"; 
            issue: "8"; pages: "840-6"
COMMENT     Sequence Label: AB.pCCL.sin.cPPT.GFP.miR-301b.sensor.PGK.dNGFR...
FEATURES             Location/Qualifiers
     source          1..925
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    160..294
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(197..216)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(616..637)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 aaattacaaa aattcaaaat tttatcgatc acgagactag cctcgagaga tctgatcata
       61 atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc
      121 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat
      181 aatggttaca aataaggcaa tagcatcaca aatttcacaa ataaggcatt tttttcactg
      241 cattctagtt ttggtttgtc caaactcatc aatgtatctt atcatgtctg gatctcaaat
      301 ccctcggaag ctgcgcctgt catcgaattc ctgcagcccg gtgcatgact aagctagtac
      361 cggtcagtgc aatgatattg tcaaagcagt gcaatgatat tgtcaaagca gtgcaatgat
      421 attgtcaaag cagtgcaatg atattgtcaa agcagtgcaa tgatattgtc aaagcagtgc
      481 aatgatattg tcaaaggcta cgtcgaccag tactccccag ttaaggtcgg ctagctcagt
      541 tagcctcccc catctctcga cgcggccgct ttacttgtac agctcgtcca tgccgagagt
      601 gatcccggcg gcggtcacga actccagcag gaccatgtga tcgcgcttct cgttggggtc
      661 tttgctcagg gcggactggg tgctcaggta gtggttgtcg ggcagcagca cggggccgtc
      721 gccgatgggg gtgttctgct ggtagtggtc ggcgagctgc acgctgccgt cctcgatgtt
      781 gtggcggatc ttgaagttca ccttgatgcc gttcttctgc ttgtcggcca tgatatagac
      841 gttgtggctg ttgtagttgt actccagctt gtgccccagg atgttgccgt cctccttgaa
      901 gtcgatgccc ttcagctcga tgcgg
//
