LOCUS       sequence-167149-         965 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Sensor Plsmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 965)
  AUTHORS   Mullokandov G, Baccarini A, Ruzo A, Jayaprakash AD, Tung N, Israelow
            B, Evans MJ, Sachidanandam R, Brown BD
  TITLE     High-throughput assessment of microRNA activity and function using 
            microRNA sensor and decoy libraries.
  JOURNAL   Nat Methods. 2012 Jul 1;9(8):840-6. doi: 10.1038/nmeth.2078.
  PUBMED    22751203
REFERENCE   2  (bases 1 to 965)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-07-1- 1"; volume: "9"; 
            issue: "8"; pages: "840-6"
COMMENT     Sequence Label: 
            AB.pCCL.sin.cPPT.GFP.miR-33a-3p.sensor.PGK.dNGFR.WPRE
FEATURES             Location/Qualifiers
     source          1..965
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    141..275
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(178..197)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(597..618)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     946..965
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ttttatcgat cacgagacta gcctcgagag atctgatcat aatcagccat accacatttg
       61 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
      121 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaggca
      181 atagcatcac aaatttcaca aataaggcat ttttttcact gcattctagt tttggtttgt
      241 ccaaactcat caatgtatct tatcatgtct ggatctcaaa tccctcggaa gctgcgcctg
      301 tcatcgaatt cctgcagccc ggtgcatgac taagctagta ccggtcaatg tttccacagt
      361 gcatcaccaa tgtttccaca gtgcatcacc aatgtttcca cagtgcatca ccaatgtttc
      421 cacagtgcat caccaatgtt tccacagtgc atcaccaatg tttccacagt gcatcacgct
      481 acgtcgacca gtactcccca gttaaggtcg gctagctcag ttagcctccc ccatctctcg
      541 acgcggccgc tttacttgta cagctcgtcc atgccgagag tgatcccggc ggcggtcacg
      601 aactccagca ggaccatgtg atcgcgcttc tcgttggggt ctttgctcag ggcggactgg
      661 gtgctcaggt agtggttgtc gggcagcagc acggggccgt cgccgatggg ggtgttctgc
      721 tggtagtggt cggcgagctg cacgctgccg tcctcgatgt tgtggcggat cttgaagttc
      781 accttgatgc cgttcttctg cttgtcggcc atgatataga cgttgtggct gttgtagttg
      841 tactccagct tgtgccccag gatgttgccg tcctccttga agtcgatgcc cttcagctcg
      901 atgcggttca ccagggtgtc gccctcgaac ttcacctcgg cgcgggtctt gtagttgccg
      961 tcgtc
//
