LOCUS       sequence-167152-         898 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Sensor Plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Mullokandov G, Baccarini A, Ruzo A, Jayaprakash AD, Tung N, Israelow
            B, Evans MJ, Sachidanandam R, Brown BD
  TITLE     High-throughput assessment of microRNA activity and function using 
            microRNA sensor and decoy libraries.
  JOURNAL   Nat Methods. 2012 Jul 1;9(8):840-6. doi: 10.1038/nmeth.2078.
  PUBMED    22751203
REFERENCE   2  (bases 1 to 898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-07-1- 1"; volume: "9"; 
            issue: "8"; pages: "840-6"
COMMENT     Sequence Label: 
            AB.pCCL.sin.cPPT.GFP.miR-652-3p.sensor.PGK.dNGFR.WPRE
FEATURES             Location/Qualifiers
     source          1..898
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    89..223
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(126..145)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(539..560)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
       61 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
      121 ataaggcaat agcatcacaa atttcacaaa taaggcattt ttttcactgc attctagttt
      181 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atctcaaatc cctcggaagc
      241 tgcgcctgtc atcgaattcc tgcagcccgg tgcatgacta agctagtacc ggtaatggcg
      301 ccactagggt tgtgaatggc gccactaggg ttgtgaatgg cgccactagg gttgtgaatg
      361 gcgccactag ggttgtgaat ggcgccacta gggttgtgaa tggcgccact agggttgtgg
      421 ctacgacgat tggtactccc cagttaaggt cggctagctc agttagcctc ccccatctct
      481 cgacgcggcc gctttacttg tacagctcgt ccatgccgag agtgatcccg gcggcggtca
      541 cgaactccag caggaccatg tgatcgcgct tctcgttggg gtctttgctc agggcggact
      601 gggtgctcag gtagtggttg tcgggcagca gcacggggcc gtcgccgatg ggggtgttct
      661 gctggtagtg gtcggcgagc tgcacgctgc cgtcctcgat gttgtggcgg atcttgaagt
      721 tcaccttgat gccgttcttc tgcttgtcgg ccatgatata gacgttgtgg ctgttgtagt
      781 tgtactccag cttgtgcccc aggatgttgc cgtcctcctt gaagtcgatg cccttcagct
      841 cgatgcggtt caccagggtg tcgccctcga acttcacctc ggcgcgggtc ttgtagtt
//
