LOCUS       sequence-167154-         955 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Sensor Plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 955)
  AUTHORS   Mullokandov G, Baccarini A, Ruzo A, Jayaprakash AD, Tung N, Israelow
            B, Evans MJ, Sachidanandam R, Brown BD
  TITLE     High-throughput assessment of microRNA activity and function using 
            microRNA sensor and decoy libraries.
  JOURNAL   Nat Methods. 2012 Jul 1;9(8):840-6. doi: 10.1038/nmeth.2078.
  PUBMED    22751203
REFERENCE   2  (bases 1 to 955)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-07-1- 1"; volume: "9"; 
            issue: "8"; pages: "840-6"
COMMENT     Sequence Label: AB.pCCL.sin.cPPT.GFP.miR-583.sensor.PGK.dNGFR....
FEATURES             Location/Qualifiers
     source          1..955
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    136..270
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(173..192)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(586..607)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     935..954
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tcgatcacga gactagcctc gagagatctg atcataatca gccataccac atttgtagag
       61 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat
      121 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aggcaatagc
      181 atcacaaatt tcacaaataa ggcatttttt tcactgcatt ctagttttgg tttgtccaaa
      241 ctcatcaatg tatcttatca tgtctggatc tcaaatccct cggaagctgc gcctgtcatc
      301 gaattcctgc agcccggtgc atgactaagc tagtaccggt caaagaggaa ggtcccatta
      361 ccaaagagga aggtcccatt accaaagagg aaggtcccat taccaaagag gaaggtccca
      421 ttaccaaaga ggaaggtccc attaccaaag aggaaggtcc cattacgcta cgacgattgg
      481 tactccccag ttaaggtcgg ctagctcagt tagcctcccc catctctcga cgcggccgct
      541 ttacttgtac agctcgtcca tgccgagagt gatcccggcg gcggtcacga actccagcag
      601 gaccatgtga tcgcgcttct cgttggggtc tttgctcagg gcggactggg tgctcaggta
      661 gtggttgtcg ggcagcagca cggggccgtc gccgatgggg gtgttctgct ggtagtggtc
      721 ggcgagctgc acgctgccgt cctcgatgtt gtggcggatc ttgaagttca ccttgatgcc
      781 gttcttctgc ttgtcggcca tgatatagac gttgtggctg ttgtagttgt actccagctt
      841 gtgccccagg atgttgccgt cctccttgaa gtcgatgccc ttcagctcga tgcggttcac
      901 cagggtgtcg ccctcgaact tcacctcggc gcgggtcttg tagttgccgt cgtcc
//
