LOCUS       sequence-167155-         925 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Sensor Plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 925)
  AUTHORS   Mullokandov G, Baccarini A, Ruzo A, Jayaprakash AD, Tung N, Israelow
            B, Evans MJ, Sachidanandam R, Brown BD
  TITLE     High-throughput assessment of microRNA activity and function using 
            microRNA sensor and decoy libraries.
  JOURNAL   Nat Methods. 2012 Jul 1;9(8):840-6. doi: 10.1038/nmeth.2078.
  PUBMED    22751203
REFERENCE   2  (bases 1 to 925)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-07-1- 1"; volume: "9"; 
            issue: "8"; pages: "840-6"
COMMENT     Sequence Label: AB.pCCL.sin.cPPT.GFP.miR-21-3p.sensor.PGK.dNGF...
FEATURES             Location/Qualifiers
     source          1..925
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    148..282
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(185..204)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(598..619)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ttcaaaattt tatcgatcac gagactagcc tcgagagatc tgatcataat cagccatacc
       61 acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa
      121 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
      181 taaggcaata gcatcacaaa tttcacaaat aaggcatttt tttcactgca ttctagtttt
      241 ggtttgtcca aactcatcaa tgtatcttat catgtctgga tctcaaatcc ctcggaagct
      301 gcgcctgtca tcgaattcct gcagcccggt gcatgactaa gctagtaccg gtcaacacca
      361 gtcgatgggc tgtcaacacc agtcgatggg ctgtcaacac cagtcgatgg gctgtcaaca
      421 ccagtcgatg ggctgtcaac accagtcgat gggctgtcaa caccagtcga tgggctgtgc
      481 tacgtcgacc agtactcccc agttaaggtc ggctagctca gttagcctcc cccatctctc
      541 gacgcggccg ctttacttgt acagctcgtc catgccgaga gtgatcccgg cggcggtcac
      601 gaactccagc aggaccatgt gatcgcgctt ctcgttgggg tctttgctca gggcggactg
      661 ggtgctcagg tagtggttgt cgggcagcag cacggggccg tcgccgatgg gggtgttctg
      721 ctggtagtgg tcggcgagct gcacgctgcc gtcctcgatg ttgtggcgga tcttgaagtt
      781 caccttgatg ccgttcttct gcttgtcggc catgatatag acgttgtggc tgttgtagtt
      841 gtactccagc ttgtgcccca ggatgttgcc gtcctccttg aagtcgatgc ccttcagctc
      901 gatgcggttc accagggtgt cgccc
//
