LOCUS       sequence-167160-        1029 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Sensor Plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Mullokandov G, Baccarini A, Ruzo A, Jayaprakash AD, Tung N, Israelow
            B, Evans MJ, Sachidanandam R, Brown BD
  TITLE     High-throughput assessment of microRNA activity and function using 
            microRNA sensor and decoy libraries.
  JOURNAL   Nat Methods. 2012 Jul 1;9(8):840-6. doi: 10.1038/nmeth.2078.
  PUBMED    22751203
REFERENCE   2  (bases 1 to 1029)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-07-1- 1"; volume: "9"; 
            issue: "8"; pages: "840-6"
COMMENT     Sequence Label: 
            AB.pCCL.sin.cPPT.GFP.miR-874-5p.sensor.PGK.dNGFR.WPRE
FEATURES             Location/Qualifiers
     source          1..1029
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    136..270
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(173..192)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(592..613)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     941..960
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tcgatcacga gactagcctc gagagatctg atcataatca gccataccac atttgtagag
       61 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat
      121 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aggcaatagc
      181 atcacaaatt tcacaaataa ggcatttttt tcactgcatt ctagttttgg tttgtccaaa
      241 ctcatcaatg tatcttatca tgtctggatc tcaaatccct cggaagctgc gcctgtcatc
      301 gaattcctgc agcccggtgc atgactaagc tagtaccggt cggccccacg caccagggta
      361 agcggcccca cgcaccaggg taagcggccc cacgcaccag ggtaagcggc cccacgcacc
      421 agggtaagcg gccccacgca ccagggtaag cggccccacg caccagggta aggctacgac
      481 gattggtact ccccagttaa ggtcggctag ctcagttagc ctcccccatc tctcgacgcg
      541 gccgctttac ttgtacagct cgtccatgcc gagagtgatc ccggcggcgg tcacgaactc
      601 cagcaggacc atgtgatcgc gcttctcgtt ggggtctttg ctcagggcgg actgggtgct
      661 caggtagtgg ttgtcgggca gcagcacggg gccgtcgccg atgggggtgt tctgctggta
      721 gtggtcggcg agctgcacgc tgccgtcctc gatgttgtgg cggatcttga agttcacctt
      781 gatgccgttc ttctgcttgt cggccatgat atagacgttg tggctgttgt agttgtactc
      841 cagcttgtgc cccaggatgt tgccgtcctc cttgaagtcg atgcccttca gctcgatgcg
      901 gttcaccagg gtgtcgccct cgaacttcac ctcggcgcgg gtcttgtagt tgccgtcgtc
      961 cttgaagaag atggtgcgct cctggacgta gccttcgggc atggcggact tgaagaagtc
     1021 gtgctgctt
//
