LOCUS       sequence-167175-         972 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Sensor Plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Mullokandov G, Baccarini A, Ruzo A, Jayaprakash AD, Tung N, Israelow
            B, Evans MJ, Sachidanandam R, Brown BD
  TITLE     High-throughput assessment of microRNA activity and function using 
            microRNA sensor and decoy libraries.
  JOURNAL   Nat Methods. 2012 Jul 1;9(8):840-6. doi: 10.1038/nmeth.2078.
  PUBMED    22751203
REFERENCE   2  (bases 1 to 972)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-07-1- 1"; volume: "9"; 
            issue: "8"; pages: "840-6"
COMMENT     Sequence Label: 
            AB.pCCL.sin.cPPT.GFP.miR-340-3p.sensor.PGK.dNGFR.WPRE
FEATURES             Location/Qualifiers
     source          1..972
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    140..274
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(177..196)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(596..617)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     945..964
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tttatcgatc acgagactag cctcgagaga tctgatcata atcagccata ccacatttgt
       61 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
      121 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaggcaa
      181 tagcatcaca aatttcacaa ataaggcatt tttttcactg cattctagtt ttggtttgtc
      241 caaactcatc aatgtatctt atcatgtctg gatctcaaat ccctcggaag ctgcgcctgt
      301 catcgaattc ctgcagcccg gtgcatgact aagctagtac cggttccgtc tcagttactt
      361 tatagctccg tctcagttac tttatagctc cgtctcagtt actttatagc tccgtctcag
      421 ttactttata gctccgtctc agttacttta tagctccgtc tcagttactt tatagcgcta
      481 cgtcgaccag tactccccag ttaaggtcgg ctagctcagt tagcctcccc catctctcga
      541 cgcggccgct ttacttgtac agctcgtcca tgccgagagt gatcccggcg gcggtcacga
      601 actccagcag gaccatgtga tcgcgcttct cgttggggtc tttgctcagg gcggactggg
      661 tgctcaggta gtggttgtcg ggcagcagca cggggccgtc gccgatgggg gtgttctgct
      721 ggtagtggtc ggcgagctgc acgctgccgt cctcgatgtt gtggcggatc ttgaagttca
      781 ccttgatgcc gttcttctgc ttgtcggcca tgatatagac gttgtggctg ttgtagttgt
      841 actccagctt gtgccccagg atgttgccgt cctccttgaa gtcgatgccc ttcagctcga
      901 tgcggttcac cagggtgtcg ccctcgaact tcacctcggc gcgggtcttg tagttgccgt
      961 cgtccttgaa ga
//
