LOCUS       sequence-167178-         913 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Host vector to fuse a gene at the N-terminus of PCNA3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 913)
  AUTHORS   Tan CY, Hirakawa H, Suzuki R, Haga T, Iwata F, Nagamune T
  TITLE     Immobilization of a Bacterial Cytochrome P450 Monooxygenase System 
            on a Solid Support.
  JOURNAL   Angew Chem Int Ed Engl. 2016 Oct 26. doi: 10.1002/anie.201608033.
  PUBMED    27781345
REFERENCE   2  (bases 1 to 913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Angew Chem 
            Int Ed Engl. 2016 Oct 26. doi: 10.1002/anie.201608033."
COMMENT     Sequence Label: pGS-3
FEATURES             Location/Qualifiers
     source          1..913
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             15..37
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             56..73
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             83..100
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 cctctagaaa taattttgtt taactttaag aaggagatat accatgggca gcagccatca
       61 tcatcatcat cacagcagcg gcctggtgcc gcgcggcagc catagagacc ggatccatga
      121 tctacctgaa atctttcgaa cgtaacatcc gcctgatcaa tatgaaagtg gtttacgatg
      181 atgttcgtgt gctgaaagat attatccagg cgctggcccg cctggttgat gaagccgtgc
      241 tgaaattcaa acaggatagc gttgaactgg tggcactgga tcgtgcgcat attagcctga
      301 tctctgttaa cctgccgcgc gaaatgttca aagaatacga tgtgaacgat gaatttaaat
      361 tcggtttcaa cacccagtac ctgatgaaaa tcctgaaagt tgcaaaacgt aaagaagcca
      421 ttgaaatcgc aagtgaaagc ccggatagtg tgattatcaa cattatcggc agcaccaatc
      481 gtgaatttaa cgttcgcaat ctggaagtgt ctgaacagga aattccggaa atcaacctgc
      541 agttcgatat tagtgcgacg atcagctctg atggttttaa aagtgccatc agcgaagttt
      601 ctaccgtgac ggataatgtg gttgtggaag gtcacgaaga tcgcattctg atcaaagcag
      661 aaggcgaaag cgaagttgaa gtggaatttt ctaaagatac cggcggtctg caggatctgg
      721 aatttagtaa agaaagcaaa aattcttaca gtgcggaata cctggatgat gttctgtctc
      781 tgacgaaact gagtgattac gtgaaaatca gcttcggcaa ccagaaaccg ctgcagctgt
      841 ttttcaatat ggaaggcggt ggcaaagtta cgtacctgct ggcgccgaaa gtgtgagatc
      901 cgaattcgag ctc
//
