LOCUS       sequence-167179-        1047 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Host vector to fuse a gene at the N-terminus of PCNA3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Tan CY, Hirakawa H, Suzuki R, Haga T, Iwata F, Nagamune T
  TITLE     Immobilization of a Bacterial Cytochrome P450 Monooxygenase System 
            on a Solid Support.
  JOURNAL   Angew Chem Int Ed Engl. 2016 Oct 26. doi: 10.1002/anie.201608033.
  PUBMED    27781345
REFERENCE   2  (bases 1 to 1047)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Angew Chem 
            Int Ed Engl. 2016 Oct 26. doi: 10.1002/anie.201608033."
COMMENT     Sequence Label: pGS-3
FEATURES             Location/Qualifiers
     source          1..1047
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(35..52)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(894..911)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(921..938)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             957..979
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    994..1018
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(1018..1037)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1019..1037)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cttcctttcg ggctttgtta gcagccggat ctcagtggtg gtggtggtgg tgctcgagtg
       61 cggccgcaag cttgtcgacg gagctcgaat tcggatctca cactttcggc gccagcaggt
      121 acgtaacttt gccaccgcct tccatattga aaaacagctg cagcggtttc tggttgccga
      181 agctgatttt cacgtaatca ctcagtttcg tcagagacag aacatcatcc aggtattccg
      241 cactgtaaga atttttgctt tctttactaa attccagatc ctgcagaccg ccggtatctt
      301 tagaaaattc cacttcaact tcgctttcgc cttctgcttt gatcagaatg cgatcttcgt
      361 gaccttccac aaccacatta tccgtcacgg tagaaacttc gctgatggca cttttaaaac
      421 catcagagct gatcgtcgca ctaatatcga actgcaggtt gatttccgga atttcctgtt
      481 cagacacttc cagattgcga acgttaaatt cacgattggt gctgccgata atgttgataa
      541 tcacactatc cgggctttca cttgcgattt caatggcttc tttacgtttt gcaactttca
      601 ggattttcat caggtactgg gtgttgaaac cgaatttaaa ttcatcgttc acatcgtatt
      661 ctttgaacat ttcgcgcggc aggttaacag agatcaggct aatatgcgca cgatccagtg
      721 ccaccagttc aacgctatcc tgtttgaatt tcagcacggc ttcatcaacc aggcgggcca
      781 gcgcctggat aatatctttc agcacacgaa catcatcgta aaccactttc atattgatca
      841 ggcggatgtt acgttcgaaa gatttcaggt agatcatgga tccggtctct atggctgccg
      901 cgcggcacca ggccgctgct gtgatgatga tgatgatggc tgctgcccat ggtatatctc
      961 cttcttaaag ttaaacaaaa ttatttctag aggggaattg ttatccgctc acaattcccc
     1021 tatagtgagt cgtattaatt tcgcggg
//
