LOCUS       sequence-167181-        1019 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Host vector to fuse a gene at the N-terminus of PCNA3 variant 
            (R112C/T180C).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Tan CY, Hirakawa H, Suzuki R, Haga T, Iwata F, Nagamune T
  TITLE     Immobilization of a Bacterial Cytochrome P450 Monooxygenase System 
            on a Solid Support.
  JOURNAL   Angew Chem Int Ed Engl. 2016 Oct 26. doi: 10.1002/anie.201608033.
  PUBMED    27781345
REFERENCE   2  (bases 1 to 1019)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Angew Chem 
            Int Ed Engl. 2016 Oct 26. doi: 10.1002/anie.201608033."
COMMENT     Sequence Label: pGS-3R112C/T180C
FEATURES             Location/Qualifiers
     source          1..1019
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             19..41
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             60..77
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             87..104
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             946..963
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ttcccctcta gaaataattt tgtttaactt taagaaggag atataccatg ggcagcagcc
       61 atcatcatca tcatcacagc agcggcctgg tgccgcgcgg cagccataga gaccggatcc
      121 atgatctacc tgaaatcttt cgaacgtaac atccgcctga tcaatatgaa agtggtttac
      181 gatgatgttc gtgtgctgaa agatattatc caggcgctgg cccgcctggt tgatgaagcc
      241 gtgctgaaat tcaaacagga tagcgttgaa ctggtggcac tggatcgtgc gcatattagc
      301 ctgatctctg ttaacctgcc gcgcgaaatg ttcaaagaat acgatgtgaa cgatgaattt
      361 aaattcggtt tcaacaccca gtacctgatg aaaatcctga aagttgcaaa acgtaaagaa
      421 gccattgaaa tcgcaagtga aagcccggat agtgtgatta tcaacattat cggcagcacc
      481 aatcgtgaat ttaacgtttg caatctggaa gtgtctgaac aggaaattcc ggaaatcaac
      541 ctgcagttcg atattagtgc gacgatcagc tctgatggtt ttaaaagtgc catcagcgaa
      601 gtttctaccg tgacggataa tgtggttgtg gaaggtcacg aagatcgcat tctgatcaaa
      661 gcagaaggcg aaagcgaagt tgaagtggaa ttttctaaag attgcggcgg tctgcaggat
      721 ctggaattta gtaaagaaag caaaaattct tacagtgcgg aatacctgga tgatgttctg
      781 tctctgacga aactgagtga ttacgtgaaa atcagcttcg gcaaccagaa accgctgcag
      841 ctgtttttca atatggaagg cggtggcaaa gttacgtacc tgctggcgcc gaaagtgtga
      901 gatccgaatt cgagctccgt cgacaagctt gcggccgcac tcgagcacca ccaccaccac
      961 cactgagatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgc
//
