LOCUS       sequence-167189-         729 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses PCNA1 variant (G108C) fused to putidaredoxin in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Tan CY, Hirakawa H, Suzuki R, Haga T, Iwata F, Nagamune T
  TITLE     Immobilization of a Bacterial Cytochrome P450 Monooxygenase System 
            on a Solid Support.
  JOURNAL   Angew Chem Int Ed Engl. 2016 Oct 26. doi: 10.1002/anie.201608033.
  PUBMED    27781345
REFERENCE   2  (bases 1 to 729)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Angew Chem 
            Int Ed Engl. 2016 Oct 26. doi: 10.1002/anie.201608033."
COMMENT     Sequence Label: p1G108CP20X
FEATURES             Location/Qualifiers
     source          1..729
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(35..52)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cttcctttcg ggctttgtta gcagccggat ctcagtggtg gtggtggtgg tgctcgagtg
       61 cggccgcaag cttgtcgacg gagctcgaat tcggatcgaa ttctcaccac tgacggtccg
      121 gaacgtcaac aacgataccg tccagctccg gggtcatgat gatctggcaa gacagacgag
      181 agttcggttt cagttccgcg gtaacagatt ccagcatacc gatttcacgt tcgttagcag
      241 ccggaacttt gtcggtgaac gcttcgttca cgtacacgtg gcaggtcgcg caagacgcag
      301 aaccaccgca gtcaccaacg atgtcgtaga tgccgttaga aactgccgcc tgcatcagag
      361 aaacaccgtc cgcaacgtcc agttcgcgac gggtaccgtc gtgagaaacg taaacaactt
      421 tagacataga gccaccacct ccgggcggcg gtggaggtgg aggcggcggt ggtggcggtg
      481 gaggaggcgg tggaggtgga ggggaaccgc cacctcccag gcgcggcgca atccagaaga
      541 tcatgtgacc gccgctaact gcttccacat caattttcat cggcagattt tcaccgaaag
      601 acaccatcgt cggcgcacta aaaccacgca ggcctttcac cgcatctttg aacatttctg
      661 cgctgtagct actagaggca gaggtatcga tgctcagttc tttcagcggt ttatctttca
      721 tcagaaagg
//
