LOCUS       sequence-16719-e        1289 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1289)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1997
REFERENCE   2  (bases 1 to 1289)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: L3618
FEATURES             Location/Qualifiers
     source          1..1289
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             363..383
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(426..454)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 gntgngcctg cggtcgactc tagaggatcc ccaattcttg agacttccac tatcttctct
       61 ggcaccctcc actacgcgta ggcgtctctc gcttcgtgta ttcccgggaa gccgggttcc
      121 cgtctctccc gccgctgccg ctgccgcaca cagctttaca cctcgtagaa tccccaaaga
      181 ggggcgtggc ttgcgggtgc caacatcctc ctgccgagga agaagcaggc actcatcact
      241 cgcatcatca acctcgggat tggccaaagg acccaaaggt atgtttcgaa tgatactaac
      301 ataacataga acattttcag gaggaccctt ggagggtacc gagctcagaa aaaatgactg
      361 ctccaaagaa gaagcgtaag gtaccggtag aaaaaatgag taaaggagaa gaacttttca
      421 ctggagttgt cccaattctt gttgaattag atggtgatgt taatgggcac aaattttctg
      481 tcagtggaga gggtgaaggt gatgcaacat acggaaaact tacccttaaa tttatttgca
      541 ctactggaaa actacctgtt ccatgggtaa gtttaaacat atatatacta actaaccctg
      601 attatttaaa ttttcagcca acacttgtca ctactttctc acacggcgta caatgcttct
      661 cgagataccc agatcatatg aaacagcatg actttttcaa gagtgccatg cccgaaggtt
      721 atgtacagga aagaactata tttttcaaag atgacgggaa ctacaagaca cgtaagttta
      781 aacagttcgg tactaactaa ccatacatat ttaaattttc aggtgctgaa gtcaagtttg
      841 aaggtgatac ccttgttaat agaatcgagt taaaaggtat tgattttaaa gaagatggaa
      901 acattcttgg acacaaattg gaatacaact tcnactcaca caacgtatac atcatggcag
      961 acaaacaaaa gaatggaatc aaagttgtaa gtttaaacat gattttacta actaactaat
     1021 ctgatttaat tttcagaact ncaaattaga cncacattga aaangnagcg ttcactagca
     1081 aaccttntca caaaatactc cattggngan ggcctgtcnt ttnacnnana ncattacntn
     1141 nccccnatct gncctttcaa natccacnaa aaaaaaaccn ctggncctct nganttgnaa
     1201 cccgcgggna tacnnngggn gggaaaaana naaggccngg ggggnaaaaa aaaaacccnc
     1261 aaagnnccga atnncccgtt tnncccttg
//
