LOCUS       sequence-167190-         970 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Sensor Plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 970)
  AUTHORS   Mullokandov G, Baccarini A, Ruzo A, Jayaprakash AD, Tung N, Israelow
            B, Evans MJ, Sachidanandam R, Brown BD
  TITLE     High-throughput assessment of microRNA activity and function using 
            microRNA sensor and decoy libraries.
  JOURNAL   Nat Methods. 2012 Jul 1;9(8):840-6. doi: 10.1038/nmeth.2078.
  PUBMED    22751203
REFERENCE   2  (bases 1 to 970)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-07-1- 1"; volume: "9"; 
            issue: "8"; pages: "840-6"
COMMENT     Sequence Label: 
            AB.pCCL.sin.cPPT.GFP.miR-181a-3p.sensor.PGK.dNGFR.WPRE
FEATURES             Location/Qualifiers
     source          1..970
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    145..279
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(182..201)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(601..622)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     950..969
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 aaaattttat cgatcacgag actagcctcg agagatctga tcataatcag ccataccaca
       61 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
      121 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
      181 ggcaatagca tcacaaattt cacaaataag gcattttttt cactgcattc tagttttggt
      241 ttgtccaaac tcatcaatgt atcttatcat gtctggatct caaatccctc ggaagctgcg
      301 cctgtcatcg aattcctgca gcccggtgca tgactaagct agtaccggta ccatcgaccg
      361 ttgattgtac caccatcgac cgttgattgt accaccatcg accgttgatt gtaccaccat
      421 cgaccgttga ttgtaccacc atcgaccgtt gattgtacca ccatcgaccg ttgattgtac
      481 cgctacgtcg accagtactc cccagttaag gtcggctagc tcagttagcc tcccccatct
      541 ctcgacgcgg ccgctttact tgtacagctc gtccatgccg agagtgatcc cggcggcggt
      601 cacgaactcc agcaggacca tgtgatcgcg cttctcgttg gggtctttgc tcagggcgga
      661 ctgggtgctc aggtagtggt tgtcgggcag cagcacgggg ccgtcgccga tgggggtgtt
      721 ctgctggtag tggtcggcga gctgcacgct gccgtcctcg atgttgtggc ggatcttgaa
      781 gttcaccttg atgccgttct tctgcttgtc ggccatgata tagacgttgt ggctgttgta
      841 gttgtactcc agcttgtgcc ccaggatgtt gccgtcctcc ttgaagtcga tgcccttcag
      901 ctcgatgcgg ttcaccaggg tgtcgccctc gaacttcacc tcggcgcggg tcttgtagtt
      961 gccgtcgtcc
//
