LOCUS       sequence-167193-         904 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Sensor Plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Mullokandov G, Baccarini A, Ruzo A, Jayaprakash AD, Tung N, Israelow
            B, Evans MJ, Sachidanandam R, Brown BD
  TITLE     High-throughput assessment of microRNA activity and function using 
            microRNA sensor and decoy libraries.
  JOURNAL   Nat Methods. 2012 Jul 1;9(8):840-6. doi: 10.1038/nmeth.2078.
  PUBMED    22751203
REFERENCE   2  (bases 1 to 904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-07-1- 1"; volume: "9"; 
            issue: "8"; pages: "840-6"
COMMENT     Sequence Label: AB.pCCL.sin.cPPT.GFP.miR-650.sensor.PGK.dNGFR....
FEATURES             Location/Qualifiers
     source          1..904
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    145..279
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(182..201)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(595..616)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 aaaattttat cgatcacgag actagcctcg agagatctga tcataatcag ccataccaca
       61 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
      121 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
      181 ggcaatagca tcacaaattt cacaaataag gcattttttt cactgcattc tagttttggt
      241 ttgtccaaac tcatcaatgt atcttatcat gtctggatct caaatccctc ggaagctgcg
      301 cctgtcatcg aattcctgca gcccggtgca tgactaagct agtaccggta ggaggcagcg
      361 ctctcaggac aggaggcagc gctctcagga caggaggcag cgctctcagg acaggaggca
      421 gcgctctcag gacaggaggc agcgctctca ggacaggagg cagcgctctc aggacgctac
      481 gacgattggt actccccagt taaggtcggc tagctcagtt agcctccccc atctctcgac
      541 gcggccgctt tacttgtaca gctcgtccat gccgagagtg atcccggcgg cggtcacgaa
      601 ctccagcagg accatgtgat cgcgcttctc gttggggtct ttgctcaggg cggactgggt
      661 gctcaggtag tggttgtcgg gcagcagcac ggggccgtcg ccgatggggg tgttctgctg
      721 gtagtggtcg gcgagctgca cgctgccgtc ctcgatgttg tggcggatct tgaagttcac
      781 cttgatgccg ttcttctgct tgtcggccat gatatagacg ttgtggctgt tgtagttgta
      841 ctccagcttg tgccccagga tgttgccgtc ctccttgaag tcgatgccct tcagctcgat
      901 gcgg
//
