LOCUS       sequence-167200-       11469 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  target  miRNA165/166  for destruction in Arabidopsis thaliana.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11469)
  AUTHORS   Tang G, Yan J, Gu Y, Qiao M, Fan R, Mao Y, Tang X
  TITLE     Construction of short tandem target mimic (STTM) to block the 
            functions of plant and animal microRNAs.
  JOURNAL   Methods. 2012 Oct;58(2):118-25. doi: 10.1016/j.ymeth.2012.10.006. 
            Epub 2012 Oct 23.
  PUBMED    23098881
REFERENCE   2  (bases 1 to 11469)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ymeth.2012.10.006"; journalName: "Methods"; date: 
            "2012-10"; volume: "58"; issue: "2"; pages: "118-25"
COMMENT     Sequence Label: pFGC5941-PacI-ath-STTM165/166-48nt
FEATURES             Location/Qualifiers
     source          1..11469
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..346
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     289..309
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     polyA_signal    507..683
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(1245..1263)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1246..1263)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             complement(1328..1987)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKGGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1902..1921
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(2024..2029,2030..2046,2047..2052))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     promoter        2326..2706
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     CDS             2712..3263
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     terminator      3273..3525
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     misc_feature    3611..3635
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             4060..4854
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      4941..5529
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5683..5700
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    5715..5855
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(5781..5800)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5941..5963
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      6199..6393
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(6459..7532)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(7961..8590)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    9891..9915
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     10103..10125
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10117..10134
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10118..10134
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(10159..10866)
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     primer_bind     11044..11063
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        11044..11062
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tgagactttt caacaaaggg taatttcggg aaacctcctc ggcttccatt gcccagctgt
       61 ctgtcacttc atcgaaagga cagtagaaaa ggaaggtggc tcctacaaat gccatcattg
      121 cgataaagga aaggctatca ttcaagatgc ctctgccgac agtggtccca aagatggacc
      181 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
      241 ggattgatgt gacatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca
      301 agacccttcc tctatataag gaagttcatt tcatttggag aggacagccc aagcttgggg
      361 gatgaagcta cctggtccga gttgttgttg ttatggtcta atttaaatat ggtctaaaga
      421 agaagaatgg ggaatgaagc tacctggtcc gagaattcgg tacgctgaaa tcaccagtct
      481 ctctctacaa atctatctct ctctattttc tccataaata atgtgtgagt agtttcccga
      541 taagggaaat tagggttctt atagggtttc gctcatgtgt tgagcatata agaaaccctt
      601 agtatgtatt tgtatttgta aaatacttct atcaataaaa tttctaattc ctaaaaccaa
      661 aatccagtac taaaatccag atctcctaaa gtccctatag atctttgtcg tgaatataaa
      721 ccagacacga gacgactaaa cctggagccc agacgccgtt cgaagctaga agtaccgctt
      781 aggcaggagg ccgttaggga aaagatgcta aggcagggtt ggttacgttg actcccccgt
      841 aggtttggtt taaatatgag gaagtggacg gaaggaagga ggaagacaag gaaggataag
      901 gttgcaggcc ctgcgcaagg taagaagatg gaaatttgat agaggtacgc tactatactt
      961 atactatacg ctaagggaat gcttgtattt ataccctata ccccctaata accccttatc
     1021 aatttaagaa ataatccgca taagcccccg cttaaaaatt ggtatcagag ccatgaatag
     1081 gtctatgacc aaaactcaag aggataaaac ctcaccaaaa tacgaaagag ttcttaactc
     1141 taaagataaa agatctttca agatcaaaac tagttccctc acaccggtga cggggatcgc
     1201 atgcgatctc tagaccatca cattggggct tcagctgctc gagttctata gtgtcaccta
     1261 aatcgtatgt gtatgataca taaggttatg tattaattgt agccgcgttc taacgtcgac
     1321 gtaccaatta cgccccgccc tgccactcat cgcagtactg ttgtaattca ttaagcattc
     1381 tgccgacatg gaagccatca caaacggcat gatgaacctg aatcgccagc ggcatcagca
     1441 ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac gggggcgaag aagttgtcca
     1501 tattggctac gtttaaatca aaactggtga aactcaccca gggattggct gagacgaaaa
     1561 acatattctc aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat
     1621 cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagcgatg
     1681 aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatatca
     1741 ccagctcacc gcctttcatt gccatacgga actccggatg agcattcatc aggcgggcaa
     1801 gaatgtgaat aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg
     1861 ccgtaatatc cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct
     1921 caaaatgttc tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttttt
     1981 tctccatttt agctccctta gctcctgaaa atctcgataa ctcattaaaa cttatcgatg
     2041 ataagctgtc aaacatgaga atttagcccg cctaatgagc gggctttttg ttgtgatccg
     2101 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     2161 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     2221 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     2281 ttaattaact ctctagactc acctaggatc catgaattct gagatttttc aaatcagtgc
     2341 gcaagacgtg acgtaagtat ccgagtcagt ttttattttt ctactaattt ggtcgtttat
     2401 ttcggcgtgt aggacatggc aaccgggcct gaatttcgcg ggtattctgt ttctattcca
     2461 actttttctt gatccgcagc cattaacgac ttttgaatag atacgctgac acgccaagcc
     2521 tcgctagtca aaagtgtacc aaacaacgct ttacagcaag aacggaatgc gcgtgacgct
     2581 cgcggtgacg ccatttcgcc ttttcagaaa tggataaata gccttgcttc ctattatatc
     2641 ttcccaaatt accaatacat tacactagca tctgaatttc ataaccaatc tcgatacacc
     2701 aaatcgaatc gatgagccca gaacgacgcc cggccgacat ccgccgtgcc accgaggcgg
     2761 acatgccggc ggtctgcacc atcgtcaacc actacatcga gacaagcacg gtcaacttcc
     2821 gtaccgagcc gcaggaaccg caggagtgga cggacgacct cgtccgtctg cgggagcgct
     2881 atccctggct cgtcgccgag gtggacggcg aggtcgccgg catcgcctac gcgggcccct
     2941 ggaaggcacg caacgcctac gactggacgg ccgagtcgac cgtgtacgtc tccccccgcc
     3001 accagcggac gggactgggc tccacgctct acacccacct gctgaagtcc ctggaggcac
     3061 agggcttcaa gagcgtggtc gctgtcatcg ggctgcccaa cgacccgagc gtgcgcatgc
     3121 acgaggcgct cggatatgcc ccccgcggca tgctgcgggc ggccggcttc aagcacggga
     3181 actggcatga cgtgggtttc tggcagctgg acttcagcct gccggtaccg ccccgtccgg
     3241 tcctgcccgt caccgagatt tgactgcaga atcttggact cccatgttgg caaaggcaac
     3301 caaacaaaca atgaatgatc cgctcctgca tatggggcgg tttgagtatt tcaactgcca
     3361 tttgggctga attgaagaca tgctcctgtc agaaattccg tgatcttact caatattcag
     3421 taatctcggc caatatccta aatgtgcgtg gctttatctg tctttgtatt gtttcatcaa
     3481 ttcatgtaac gtttgctttt cttatgaatt ttcaaataaa ttatcagatc cgagctcgaa
     3541 ttaattcggc gttaattcag tacattaaaa acgtccgcaa tgtgttatta agttgtctaa
     3601 gcgtcaattt gtttacacca caatatatcc tgccaccagc cagccaacag ctccccgacc
     3661 ggcagctcgg cacaaaatca ccactcgata caggcagccc atcagtccgg gacggcgtca
     3721 gcgggagagc cgttgtaagg cggcagactt tgctcatgtt accgatgcta ttcggaagaa
     3781 cggcaactaa gctgccgggt ttgaaacacg gatgatctcg cggagggtag catgttgatt
     3841 gtaacgatga cagagcgttg ctgcctgtga tcaccgcggt ttcaaaatcg gctccgtcga
     3901 tactatgtta tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag
     3961 gttttagaat gcaaggaaca gtgaattgga gttcgtcttg ttataattag cttcttgggg
     4021 tatctttaaa tactgtagaa aagaggaagg aaataataaa tggctaaaat gagaatatca
     4081 ccggaattga aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct
     4141 cctgctaagg tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac
     4201 agccggtata aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg
     4261 gaaggaaagc tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat
     4321 ctgctcatga gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc
     4381 cctgaaaaga ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata
     4441 tcggattgtc cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg
     4501 aataacgatc tggccgatgt ggattgcgaa aactgggaag aagacactcc atttaaagat
     4561 ccgcgcgagc tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc
     4621 cacggcgacc tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt
     4681 gatcttggga gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg
     4741 atcagggagg atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc
     4801 aagcctgatt gggagaaaat aaaatattat attttactgg atgaattgtt ttagtaccta
     4861 gaatgcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     4921 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     4981 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     5041 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
     5101 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     5161 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     5221 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     5281 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     5341 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     5401 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     5461 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     5521 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     5581 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     5641 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     5701 gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
     5761 cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca
     5821 ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg
     5881 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
     5941 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagggtg
     6001 ccttgatgtg ggcgccggcg gtcgagtggc gacggcgcgg cttgtccgcg ccctggtaga
     6061 ttgcctggcc gtaggccagc catttttgag cggccagcgg ccgcgatagg ccgacgcgaa
     6121 gcggcggggc gtagggagcg cagcgaccga agggtaggcg ctttttgcag ctcttcggct
     6181 gtgcgctggc cagacagtta tgcacaggcc aggcgggttt taagagtttt aataagtttt
     6241 aaagagtttt aggcggaaaa atcgcctttt ttctctttta tatcagtcac ttacatgtgt
     6301 gaccggttcc caatgtacgg ctttgggttc ccaatgtacg ggttccggtt cccaatgtac
     6361 ggctttgggt tcccaatgta cgtgctatcc acaggaaaga gaccttttcg acctttttcc
     6421 cctgctaggg caatttgccc tagcatctgc tccgtacatt aggaaccggc ggatgcttcg
     6481 ccctcgatca ggttgcggta gcgcatgact aggatcgggc cagcctgccc cgcctcctcc
     6541 ttcaaatcgt actccggcag gtcatttgac ccgatcagct tgcgcacggt gaaacagaac
     6601 ttcttgaact ctccggcgct gccactgcgt tcgtagatcg tcttgaacaa ccatctggct
     6661 tctgccttgc ctgcggcgcg gcgtgccagg cggtagagaa aacggccgat gccgggatcg
     6721 atcaaaaagt aatcggggtg aaccgtcagc acgtccgggt tcttgccttc tgtgatctcg
     6781 cggtacatcc aatcagctag ctcgatctcg atgtactccg gccgcccggt ttcgctcttt
     6841 acgatcttgt agcggctaat caaggcttca ccctcggata ccgtcaccag gcggccgttc
     6901 ttggccttct tcgtacgctg catggcaacg tgcgtggtgt ttaaccgaat gcaggtttct
     6961 accaggtcgt ctttctgctt tccgccatcg gctcgccggc agaacttgag tacgtccgca
     7021 acgtgtggac ggaacacgcg gccgggcttg tctcccttcc cttcccggta tcggttcatg
     7081 gattcggtta gatgggaaac cgccatcagt accaggtcgt aatcccacac actggccatg
     7141 ccggccggcc ctgcggaaac ctctacgtgc ccgtctggaa gctcgtagcg gatcacctcg
     7201 ccagctcgtc ggtcacgctt cgacagacgg aaaacggcca cgtccatgat gctgcgacta
     7261 tcgcgggtgc ccacgtcata gagcatcgga acgaaaaaat ctggttgctc gtcgcccttg
     7321 ggcggcttcc taatcgacgg cgcaccggct gccggcggtt gccgggattc tttgcggatt
     7381 cgatcagcgg ccgcttgcca cgattcaccg gggcgtgctt ctgcctcgat gcgttgccgc
     7441 tgggcggcct gcgcggcctt caacttctcc accaggtcat cacccagcgc cgcgccgatt
     7501 tgtaccgggc cggatggttt gcgaccgctc acgccgattc ctcgggcttg ggggttccag
     7561 tgccattgca gggccggcag gcaacccagc cgcttacgcc tggccaaccg cccgttcctc
     7621 cacacatggg gcattccacg gcgtcggtgc ctggttgttc ttgattttcc atgccgcctc
     7681 ctttagccgc taaaattcat ctactcattt attcatttgc tcatttactc tggtagctgc
     7741 gcgatgtatt cagatagcag ctcggtaatg gtcttgcctt ggcgtaccgc gtacatcttc
     7801 agcttggtgt gatcctccgc cggcaactga aagttgaccc gcttcatggc tggcgtgtct
     7861 gccaggctgg ccaacgttgc agccttgctg ctgcgtgcgc tcggacggcc ggcacttagc
     7921 gtgtttgtgc ttttgctcat tttctcttta cctcattaac tcaaatgagt tttgatttaa
     7981 tttcagcggc cagcgcctgg acctcgcggg cagcgtcgcc ctcgggttct gattcaagaa
     8041 cggttgtgcc ggcggcggca gtgcctgggt agctcacgcg ctgcgtgata cgggactcaa
     8101 gaatgggcag ctcgtacccg gccagcgcct cggcaacctc accgccgatg cgcgtgcctt
     8161 tgatcgcccg cgacacgaca aaggccgctt gtagccttcc atccgtgacc tcaatgcgct
     8221 gcttaaccag ctccaccagg tcggcggtgg cccatatgtc gtaagggctt ggctgcaccg
     8281 gaatcagcac gaagtcggct gccttgatcg cggacacagc caagtccgcc gcctggggcg
     8341 ctccgtcgat cactacgaag tcgcgccggc cgatggcctt cacgtcgcgg tcaatcgtcg
     8401 ggcggtcgat gccgacaacg gttagcggtt gatcttcccg cacggccgcc caatcgcggg
     8461 cactgccctg gggatcggaa tcgactaaca gaacatcggc cccggcgagt tgcagggcgc
     8521 gggctagatg ggttgcgatg gtcgtcttgc ctgacccgcc tttctggtta agtacagcga
     8581 taaccttcat gcgttcccct tgcgtatttg tttatttact catcgcatca tatacgcagc
     8641 gaccgcatga cgcaagctgt tttactcaaa tacacatcac ctttttagac ggcggcgctc
     8701 ggtttcttca gcggccaagc tggccggcca ggccgccagc ttggcatcag acaaaccggc
     8761 caggatttca tgcagccgca cggttgagac gtgcgcgggc ggctcgaaca cgtacccggc
     8821 cgcgatcatc tccgcctcga tctcttcggt aatgaaaaac ggttcgtcct ggccgtcctg
     8881 gtgcggtttc atgcttgttc ctcttggcgt tcattctcgg cggccgccag ggcgtcggcc
     8941 tcggtcaatg cgtcctcacg gaaggcaccg cgccgcctgg cctcggtggg cgtcacttcc
     9001 tcgctgcgct caagtgcgcg gtacagggtc gagcgatgca cgccaagcag tgcagccgcc
     9061 tctttcacgg tgcggccttc ctggtcgatc agctcgcggg cgtgcgcgat ctgtgccggg
     9121 gtgagggtag ggcgggggcc aaacttcacg cctcgggcct tggcggcctc gcgcccgctc
     9181 cgggtgcggt cgatgattag ggaacgctcg aactcggcaa tgccggcgaa cacggtcaac
     9241 accatgcggc cggccggcgt ggtggtgtcg gcccacggct ctgccaggct acgcaggccc
     9301 gcgccggcct cctggatgcg ctcggcaatg tccagtaggt cgcgggtgct gcgggccagg
     9361 cggtctagcc tggtcactgt cacaacgtcg ccagggcgta ggtggtcaag catcctggcc
     9421 agctccgggc ggtcgcgcct ggtgccggtg atcttctcgg aaaacagctt ggtgcagccg
     9481 gccgcgtgca gttcggcccg ttggttggtc aagtcctggt cgtcggtgct gacgcgggca
     9541 tagcccagca ggccagcggc ggcgctcttg ttcatggcgt aatgtctccg gttctagtcg
     9601 caagtattct actttatgcg actaaaacac gcgacaagaa aacgccagga aaagggcagg
     9661 gcggcagcct gtcgcgtaac ttaggacttg tgcgacatgt cgttttcaga agacggctgc
     9721 actgaacgtc agaagccgac tgcactatag cagcggaggg gttggatcaa agtactttga
     9781 tcccgagggg aaccctgtgg ttggcatgca catacaaatg gacgaacgga taaacctttt
     9841 cacgcccttt taaatatccg attattctaa taaacgctct tttctcttag gtttacccgc
     9901 caatatatcc tgtcaaacac tgatagttta aactgaaggc gggaaacgac aatctgatcc
     9961 aagctcaagc tgctctagca ttcgccattc aggctgcgca actgttggga agggcgatcg
    10021 gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta
    10081 agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgccaag
    10141 cttgcatgcc tgcaggtcct gctgagcctc gacatgttgt cgcaaaattc gccctggacc
    10201 cgcccaacga tttgtcgtca ctgtcaaggt ttgacctgca cttcatttgg ggcccacata
    10261 caccaaaaaa atgctgcata attctcgggg cagcaagtcg gttacccggc cgccgtgctg
    10321 gaccgggttg aatggtgccc gtaactttcg gtagagcgga cggccaatac tcaacttcaa
    10381 ggaatctcac ccatgcgcgc cggcggggaa ccggagttcc cttcagtgag cgttattagt
    10441 tcgccgctcg gtgtgtcgta gatactagcc cctggggcac ttttgaaatt tgaataagat
    10501 ttatgtaatc agtcttttag gtttgaccgg ttctgccgct ttttttaaaa ttggatttgt
    10561 aataataaaa cgcaattgtt tgttattgtg gcgctctatc atagatgtcg ctataaacct
    10621 attcagcaca atatattgtt ttcattttaa tattgtacat ataagtagta gggtacaatc
    10681 agtaaattga acggagaata ttattcataa aaatacgata gtaacgggtg atatattcat
    10741 tagaatgaac cgaaaccggc ggtaaggatc tgagctacac atgctcaggt tttttacaac
    10801 gtgcacaaca gaattgaaag caaatatcat gcgatcatag gcgtctcgca tatctcatta
    10861 aagcaggact ctagggacta gtcccgggtc ttaattaagt ttgcaagcag cagattacgc
    10921 gcagaaaaaa aggatctcaa gaagatcctt tggatcacaa aaaaaagccc gctcattagg
    10981 cgggttaaat tcccgggtac tgcagtcgat tcattaatgc aggttaacct ggcttatcga
    11041 aattaatacg actcactata gggagaccgg cagatctgat ccctactcca aaaatgtcaa
    11101 agatacagtc tcagaagacc aaagggctat tgagactttt caacaaaggg taatttcggg
    11161 aaacctcctc ggattccatt gcccagctat ctgtcacttc atcgaaagga cagtagaaaa
    11221 ggaaggtggc tcctacaaat gccatcattg cgataaagga aaggctatca ttcaagatgc
    11281 ctctgccgac agtggtccca aagatggacc cccacccacg aggagcatcg tggaaaaaga
    11341 agacgttcca accacgtctt caaagcaagt ggattgatgt gacatctcca ctgacgtaag
    11401 ggatgacgca caatcccacc cctactccaa aaatgtcaaa gatacagtct cagaagacca
    11461 aagggctat
//
