LOCUS       sequence-167203-        1042 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  2nd Pro5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Tan CY, Hirakawa H, Suzuki R, Haga T, Iwata F, Nagamune T
  TITLE     Immobilization of a Bacterial Cytochrome P450 Monooxygenase System 
            on a Solid Support.
  JOURNAL   Angew Chem Int Ed Engl. 2016 Oct 26. doi: 10.1002/anie.201608033.
  PUBMED    27781345
REFERENCE   2  (bases 1 to 1042)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Angew Chem 
            Int Ed Engl. 2016 Oct 26. doi: 10.1002/anie.201608033."
COMMENT     Sequence Label: pHPro52
FEATURES             Location/Qualifiers
     source          1..1042
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     118..140
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     132..149
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     133..149
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(235..251)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(235..251)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(248..270)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    259..275
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(283..289,290..307,308..313))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    328..349
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
       61 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
      121 agtcacgacg ttgtaaaacg acggccagtg ccaagcttgc atgcctgcag gtcgactcta
      181 gaggatcggt ctcgtggcgg tggaggaggc ggtcgagacc aattcgtaat catggtcata
      241 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
      301 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
      361 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
      421 acgcgcgggg agaggcggtt tgcgtattgg agcttggcac tgggccaagc tgaatttctg
      481 ccattcatcc gcttattatc acttattcag gcgtagcaac caggcgttta agggcaccaa
      541 taactgcctt aaaaaaatta cgccccgccc tgccactcat cgcagtactg ttgtaattca
      601 ttaagcattc tgccgacatg gaagccatca caaacggcat gatgaacctg aatcgccagc
      661 ggcatcagca ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac gggggcgaag
      721 aagttgtcca tattggccac gtttaaatca aaactggtga aactcaccca gggattggct
      781 gagacgaaaa acatattctc aataaaccct ttagggaaat aggccaggtt ttcaccgtaa
      841 cacgccacat cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg gtattcactc
      901 cagagcgatg aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg gtgaacacta
      961 tcccatatca ccagctcacc gtctttcatt gccatacgaa attccggatg agcattcatc
     1021 aggcgggcaa gaatgtgaat aa
//
